霉茶总黄酮对自发性高血压大鼠心脏的保护作用及机制研究
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篇名: 霉茶总黄酮对自发性高血压大鼠心脏的保护作用及机制研究
TITLE:
摘要: 目的:观察霉茶总黄酮对自发性高血压大鼠(SHR)心脏的保护作用,并探讨其作用机制。方法:将50只SHR按体质量随机分为模型组、复方罗布麻片组(阳性药物,50 mg/kg)和霉茶总黄酮低、中、高剂量组(90、180、360 mg/kg),每组10只。各给药组大鼠ig相应药物,模型组大鼠ig等体积蒸馏水,每天2次,连续7周。测定大鼠血压并计算大鼠心脏指数;采用苏木精-伊红染色法观察心脏组织形态结构变化;采用实时荧光定量聚合酶链式反应法检测心脏组织中血管紧张素转化酶(ACE)、血管紧张素Ⅱ(AngⅡ)和醛固酮合成酶(CYP11B2)mRNA的表达。结果:霉茶总黄酮可明显降低SHR血压(P<0.01),改善SHR心脏组织细胞形态不规则、固缩和组织纤维增生等炎性病变,降低SHR心脏组织中ACE、AngⅡ、CYP11B2 mRNA的表达水平(P<0.05或P<0.01)。结论:霉茶总黄酮对SHR心脏有一定的保护作用,其机制可能与下调心脏组织中ACE、AngⅡ和CYP11B2 mRNA表达有关。
ABSTRACT: OBJECTIVE: To observe the protective effect of total flavonoids from Ampelopsis megalophylla on cardiac tissue of spontaneously hypertensive rats (SHR), and to investigate its mechanism. METHODS: 50 SHRs were randomly divided into model group, Compound apocynum tablets group (positive drug, 50 mg/kg) and total flavonoids from A. megalophylla low-dose, medium-dose and high-dose groups (90, 180, 360 mg/kg), with 10 rats in each group. The rats were given relevant medicine intragastrically, and model group was given constant volume of distilled water intragastrically, twice a day, for consecutive 7 weeks. Blood pressure of rats were determined and cardiac index of rats were calculated; the morphology and structure of cardiac tissue were observed by HE staining. mRNA expression of ACE, AngⅡ and CYP11B2 in cardiac tissues were determined by RT-PCR. RESULTS: Total flavonoids from A. megalophylla can significantly decrease the blood pressure of SHR (P<0.01), improve the flammatory lesions of  hearte  issue  such as  irregular cell morphology, cell shrinkage, proliferation of fibrous tissue and so on, and it also can significantly decrease the mRNA expression levels of ACE, AngⅡ, CYP11B2 in cardiac tissue of rats (P<0.05 or P<0.01). CONCLUSIONS: Total flavonoids from A. megalophylla can protect cardiac tissue of SHR, the mechanism of which may be associated with down-regulating mRNA expression of ACE, AngⅡ and CYP11B2 in cardiac tissue.
期刊: 2016年第27卷第34期
作者: 刘佩,郑国华,吴勇,谭永霞,张宝徽
AUTHORS: LIU Pei,ZHENG Guohua,WU Yong,TAN Yongxia,ZHANG Baohui
关键字: 霉茶总黄酮;自发性高血压大鼠;血管紧张素转化酶;血管紧张素Ⅱ; 醛固酮合成酶
KEYWORDS: Total flavonoids from Ampelopsis megalophylla; Spontaneous hypertensive rats; ACE; AngⅡ; CYP11B2
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