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黔产毛蒟挥发油的提取工艺优化及化学成分分析
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| 篇名： | 黔产毛蒟挥发油的提取工艺优化及化学成分分析 |
| TITLE： | |
| 摘要： | 目的：优化黔产毛蒟挥发油的提取工艺并分析其化学成分。方法 ：采用水蒸气蒸馏法（HD）提取黔产毛蒟挥发油。以挥发油提取率为考察指标，以浸泡时间、提取时间、料液比（g/ml）、粉碎程度为考察因素设计正交试验；同时进行验证试验。采用气相色谱-质谱联用法对黔产毛蒟挥发油的化学成分进行分离鉴定，并以面积归一化法测定各成分的相对百分含量。结果：最优提取工艺为浸泡时间3 h、提取时间5 h、料液比1 ∶ 10、粉碎程度12目；验证试验中挥发油平均提取率为59.83％（RSD＝0.905 3％，n＝3）。共鉴定出78种化合物，相对百分含量为81.125％；其中γ-榄香烯（11.858％）、4-乙烯基-α，4-三甲基-3蒟（1-甲基乙烯基）-环己烷甲醇（8.701％）、石竹（8.443％）、α-石竹烯（6.216％）等4种化合物为主要成分。结论：优化的HD法生产设备简单、生产成本低廉、重现性好；挥发油成分分析方法简单、可行，可对黔产毛蒟挥发油的化学成分进行分析。 |
| ABSTRACT： | OBJECTIVE： To optimize the extraction technology of volatile oil from Guizhou Piper puberulum and analyze its chemical compositions. METHODS： The volatile oil was extracted from Guizhou P. puberulum by steam distillation （HD method）. Orthogonal test was designed using the extract rate of volatile oil as index， with soaking time， extraction time， material-liquid ratio （g/ml） and smashed particle size as factors. Validation test was also conducted. GC-MS was adopted to separate and identify the chemical compositions of the volatile oil from Guizhou P. puberulum， and area normalization method was used for the determination of relative percentage of each composition. RESULTS： The optimal extraction technology was as follows as soaking time of 3 h， extraction time of 5 h， material-liquid ratio of 1 ∶ 10 and degree of grinding 12 meshes； in validation test， average extract rate of volatile oil was 59.83％（RSD＝0.905 3％，n＝3）. 78 compounds were identified， and relative percentage was 81.125％； there were four main compositions such as γ-elemene （11.858％）， 4-vinyl-α， 4-trimethyl-3-（1-methylvinyl）-cyclohexane methanol（8.701％）， carnation （8.443％） and α-caryophyllene （6.216％）. CONCLUSIONS： Optimized HD method is simple in production equipment， low in production cost， good in repeatability； determination method of validation oil is simple and feasible， and can analyze chemical compositions of volatile oil from Guizhou P. puberulum. |
| 期刊： | 2016年第27卷第31期 |
| 作者： | 杨艳，韦余，王玉和，鄢乾燕 |
| AUTHORS： | YANG Yan，WEI Yu，WANG Yuhe，YAN Qianyan |
| 关键字： | 黔产毛蒟；挥发油；水蒸气蒸馏法；气相色谱-质谱法；成分 |
| KEYWORDS： | Guizhou Piper puberulum； Volatile oil； Steam distillation method； GC-MS； Compositions |
| 阅读数： | 330 次 |
| 本月下载数： | 0 次 |
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