白及多糖对血热出血模型大鼠的止血作用及机制研究
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篇名: 白及多糖对血热出血模型大鼠的止血作用及机制研究
TITLE:
摘要: 目的:研究白及多糖(BSP)对血热出血模型大鼠的止血作用及机制。方法:将大鼠随机分为正常对照组、模型组、云南白药组(0.50 g/kg)和BSP低、中、高剂量组(0.34 、0.68、1.35 g/kg),每组10只。除正常对照组外,其余各组大鼠均采用干姜水煎液结合5%乙醇给药复制血热出血模型。造模成功后,除正常对照组和模型组大鼠ig生理盐水外,其余各组大鼠ig相应药物,每天1次,连续14 d。末次给药1 h后检测各组大鼠体质量、摄食量、饮水量、血小板聚集率、组织型纤溶酶原激活剂(t-PA)和纤溶酶原激活物抑制剂(PAI-1)的含量;检查肺、胃组织病理学变化。结果:与正常对照组比较,模型组大鼠体质量、摄食量、血小板最大聚集率、PAI-1含量均减少,饮水量、t-PA含量及t-PA/PAI-1比值均增加(P<0.05或P<0.01),肺、胃组织均表现出出血和炎症等病理变化。与模型组比较,云南白药组和BSP低、中、高剂量组大鼠体质量、摄食量均增加,饮水量减少(P<0.05或P<0.01);云南白药组和BSP中、高剂量组大鼠血小板最大聚集率、PAI-1含量均增加,t-PA/PAI-1比值减少(P<0.05或P<0.01),肺、胃组织的病理变化均不同程度减轻。结论:BSP可能通过升高血小板聚集率、调节纤溶系统、改善肺和胃组织的病变,综合发挥止血作用。
ABSTRACT: OBJECTIVE: To study the hemostatic effect and mechanism of the polysaccharides from Bletilla striata in blood heat and hemorrhage (BBH) model rats. METHODS: Rats were randomly divided into normal control group, model group, Yunnan baiyao group (0.50 g/kg), and BSP low-dose, medium-dose and high-dose groups (0.34,0.68, 1.35 g/kg), with 10 rats in each group. Except for normal control group, those groups were given water decoction of Rhizoma Zingiberis and 5% ethanol to induce BBH model. After modeling, those groups were given relevant medicine intragastrically, once a day, for 14 days, except normal control group and model group were given normal saline intragastrically. 1 h after last administration, body weight, food intake, water intake, the rate of platelet aggregation, the contents of t-PA and PAI-1, pathological change of lung and stomach were all detected in each group. RESULTS: Compared with normal control group, body weight, food intake, maximal rate of platelet aggregation and the content of PAI-1 were decreased in model group; while water intake, the content of t-PA and ratio of t-PA/PAI-1 were increased (P<0.05 or P<0.01). Moreover, hemorrhage and inflammation of lung and stomach were found. Compared with model group, the body weight and food intake of Yunnan baiyao group, BSP low-dose, medium-dose and high-dose groups were increased, while water intake were decreased (P<0.05 or P<0.01); the maximal rate of platelet aggregation and the content of PAI-1 were increased in Yunnan baiyao group, BSP medium-dose and high-dose groups, while ratio of t-PA/PAI-1 were decreased (P<0.05 or P<0.01), the pathological changes of lung and stomach relieved to varying degrees. CONCLUSIONS: BSP exerts hemostatic effect by up-regulating the rate of platelet aggregation, rising fibrinolysis systems, improving the lesion of lung and stomach.
期刊: 2016年第27卷第31期
作者: 董永喜,刘星星,董莉,王霞,黄勇,王永林
AUTHORS: DONG Yongxi,LIU Xingxing,DONG Li,WANG Xia,HUANG Yong,WANG Yonglin
关键字: 白及多糖;血热出血;大鼠;血小板聚集;纤溶系统;组织病理学检查
KEYWORDS: Polysaccharides from Bletilla striata; Blood heat and hemorrhage; Rats; Platelet aggregation; Fibrinolytic system; Histopathological examination
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