香菇多糖对溃疡性结肠炎大鼠的改善作用及其机制研究
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篇名: 香菇多糖对溃疡性结肠炎大鼠的改善作用及其机制研究
TITLE:
摘要: 目的:研究香菇多糖(LTN)对溃疡性结肠炎(UC)大鼠的改善作用及其机制。方法:将64只SD大鼠随机分为正常组(12只)和造模组(52只)。造模组大鼠采用2,4-二硝基氯苯丙酮和2,4-二硝基氯苯乙醇诱导UC模型。将造模成功的大鼠随机分为模型组、阳性组(柳氮磺胺吡啶片,300 mg/kg)和LTN低、中、高剂量组(0.2、0.4、0.8 mg/kg),每组10只。各给药组大鼠ig相应药物,正常组和模型组大鼠ig生理盐水,每天1次,连续15 d。检测外周血淋巴细胞(PBMC)中CD3+、CD4+、CD8+水平和结肠组织中肿瘤坏死因子α(TNF-α)、白细胞介素2(IL-2)、IL-4、IL-10水平,并观察结肠组织病理变化。结果:与正常组比较,模型组大鼠PBMC中CD3+、CD4+、CD8+水平和CD4+/CD8+比值以及结肠组织中IL-4、IL-10水平明显降低,结肠组织中TNF-α、IL-2水平明显升高(P<0.05),结肠组织发生明显溃疡性病变(集中于Ⅱ、Ⅲ级)。与模型组比较,各给药组大鼠PBMC中CD3+、CD4+、CD8+水平,CD4+/CD8+比值以及结肠组织中IL-4、IL-10水平明显升高,结肠组织中TNF-α、IL-2水平明显降低(P<0.05);结肠组织炎症明显减轻、溃疡形成明显改善。结论:香菇多糖对UC模型大鼠具有一定的改善作用,其机制可能与增强大鼠免疫功能有关。
ABSTRACT: OBJECTIVE: To study the regulatory effect of lentinan (LTN) on immune function in ulcerative colitis (UC) rats and its mechanism. METHODS: 64 SD rats were randomly divided into normal group (12 rats) and modeling group (52 rats). UC model was induced in modeling group by a compound method of 2,4-dinitrochlorobenzene (DNCB) acetone and 2,4-DNCB ethanol. After modeling, rats were randomly divided into model group, positive group (sulfasalazine tablet, 300 mg/kg), LTN low-dose, medium-dose and high-dose groups (0.2, 0.4, 0.8 mg/kg), with 10 rats in each group. Treatment groups were given relevant medicine intragastrically, and normal group and model group were given normal saline intragastrically, once a day, for consecutive 15 d. The levels of CD3+,CD4+,CD8+ in peripheral blood lymphocytes (PBMC) and the levels of TNF-α, IL-2, IL-4 and IL-10 in colon were all determined, and the pathological changes of colon tissue were observed. RESULTS: Compared with normal group, the levels of CD3+,CD4+,CD8+, the ratio of CD4+/CD8+, and the levels of IL-4 and IL-10 decreased significantly, while the levels of TNF-α and IL-2 in colon tissue significantly increased (P<0.05); obvious ulcerative lesion was observed in colon tissue (concentrated in grade Ⅱ and Ⅲ). Compared with model group, the levels of CD3+,CD4+,CD8+, the ratio of CD4+/CD8+, and the levels of IL-4, IL-10 increased significantly, while the levels of TNF-α, IL-2 in colon tissue significantly decreased (P<0.05); the inflammation of colon tissue was significantly reduced, and the ulcer formation was significantly improved. CONCLUSIONS: LTN has a certain improvement effect on UC model rats, and its mechanism may be related to enhancing the immune function.
期刊: 2016年第27卷第22期
作者: 周泠,唐文台,王钢
AUTHORS: ZHOU Ling,TANG Wentai,WANG Gang
关键字: 香菇多糖;溃疡性结肠炎;免疫功能;大鼠;机制
KEYWORDS: Lentinan; Ulcerative colitis; Immune function; Rat; Mechanism
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