玉屏风散对肺气虚证模型大鼠免疫功能的影响
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篇名: 玉屏风散对肺气虚证模型大鼠免疫功能的影响
TITLE:
摘要: 目的:考察玉屏风散对肺气虚证模型大鼠免疫功能的影响。方法:将60只SD大鼠随机分为正常组、模型组、阳性组[人参北芪片0.6 g(生药)/kg]和玉屏风散高、中、低剂量组[24、12、6 g(生药)/kg],每组10只。除正常组外,其余各组大鼠均采用二氧化硫+风寒综合刺激法复制肺气虚证模型。造模成功后,各给药组大鼠ig相应药液,正常组和模型组大鼠ig等体积生理盐水,每天1次,连续13 d。给药结束后,测定大鼠血清中白细胞介素(IL)-3、IL-6水平及T淋巴细胞中CD3+、CD4+、CD8+比例,观察肺、支气管组织病理变化。结果:与正常组比较,模型组大鼠血清中IL-3、IL-6水平及T淋巴细胞中CD8+比例明显升高,T淋巴细胞中CD3+、CD4+比例及CD4+/CD8+比值降低(P<0.01);肺、支气管明显损伤并伴有炎症反应。与模型组比较,阳性组和玉屏风散高剂量组大鼠血清中IL-3水平明显降低;阳性组和玉屏风散高、中剂量组大鼠血清中IL-6水平、T淋巴细胞中CD8+比例明显降低,T淋巴细胞中CD3+比例明显升高;各给药组大鼠血清中CD4+比例、CD4+/CD8+比值明显升高(P<0.05或P<0.01);肺、支气管损伤及炎症反应程度减轻。结论:玉屏风散可增强肺气虚证模型大鼠的免疫功能。
ABSTRACT: OBJECTIVE: To study the effects of Yupingfeng powder on immune function of rats with lung-qi deficiency syndrome. METHODS: 60 SD rats were randomly divided into normal group, model group, positive group [Renshen beiqi tablet 0.6 g (crude drug)/kg] and Yupingfeng powder high-dose, medium-dose and low-dose groups [24, 12, 6 g (crude drug)/kg], with 10 rats in each group. Except for normal group, those groups were given SO2+comprehensive cold stimulation to induce lung-qi deficiency syndrome model. After modeling, treatment groups were given relevant drug solutions intragastrically, and normal group and model group were given constant volume of normal saline intragastrically, once a day, for consecutive 13 d. After administration, serum levels of IL-3 and IL-6, the proportion of CD3+, CD4+, CD8+ in T lymphocyte were determined, and pathological changes of lung and bronchus were observed. RESULTS: Compared with normal group, serum levels of IL-3 and IL-6 and the proportion of CD8+ in T lymphocyte increased significantly in model group, while the proportions of CD3+ and CD4+ in T lymphocyte and the ratio of CD4+/CD8+ decreased (P<0.01); obvious lung and bronchus injury and inflammatory reaction were observed. Compared with model group, serum level of IL-3 decreased significantly in positive group and Yupingfeng powder high-dose group; serum level of IL-6 and the proportion of CD8+ in T lymphocyte decreased significantly in positive group and Yupingfeng powder high-dose and medium-dose groups, while the proportion of CD3+ in T lymphocyte increased significantly; the proportion of CD4+ and the ratio of CD4+/CD8+ increased significantly in treatment groups (P<0.05 or P<0.01); lung and bronchial injury, and inflammation reaction were relieved. CONCLUSIONS: Yupingfeng powder can enhance immune function of rats with lung-qi deficiency syndrome.
期刊: 2016年第27卷第22期
作者: 杨胜,张仲林,袁明勇,郑玲利,李燕,廖昌军
AUTHORS: YANG Sheng,ZHANG Zhonglin,YUAN Mingyong,ZHENG Lingli,LI Yan,LIAO Changjun
关键字: 玉屏风散;肺气虚证;免疫功能;T淋巴细胞;大鼠
KEYWORDS: Yupingfeng powder; Lung-qi deficiency; Immune function; T lymphocyte; Rats
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