转铁蛋白修饰长春新碱-粉防己碱脂质体中主药含量及包封率的测定
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篇名: 转铁蛋白修饰长春新碱-粉防己碱脂质体中主药含量及包封率的测定
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摘要: 目的:建立转铁蛋白修饰长春新碱-粉防己碱脂质体中主药含量及脂质体包封率的测定方法。方法:采用高效液相色谱法测定长春新碱含量,色谱柱为依利特C18,流动相为甲醇-15%三乙胺水溶液(70 ∶ 30),流速为1.0 ml/min,检测波长为297 nm,柱温为30 ℃,进样量为20 μl;采用葡聚糖凝胶柱色谱法分离脂质体与游离药物,计算包封率。结果:长春新碱检测质量浓度为160~1 600 μg/ml(r=0.999 8,n=5),平均加样回收率为99.20%(RSD=0.26%,n=9),精密度RSD为0.070%(n=5)。脂质体中长春新碱的平均含量为0.790 mg/ml(RSD=0.15%,n=3),平均包封率为85.94%(RSD=2.08%,n=3)。结论:该方法准确可靠、简单快速,可用于转铁蛋白修饰长春新碱-粉防己碱脂质体中长春新碱的含量及包封率测定。
ABSTRACT: OBJECTIVE: To establish the method for the determination of main components content and entrapment efficiency of transferrin modified vincristine-tetrandrine liposomes. METHODS: HPLC method was adopted to determine the content of vincristine. The determination was performed on ELITE C18 column with mobile phase consisted of methanol-15% triethylamine  (70 ∶ 30) at a flow rate of 1.0 ml/min. The determination wavelength was set at 297 nm and column temperature was 30 ℃. The sample size was 20 μl. Free drug was isolated from liposomes by dextran gel column chromatography, and entrapment efficiency was determined. RESULTS: The linear range of vincristine was 160-1 600 μg/ml (r=0.999 8, n=5) with average recovery of 99.20% (RSD=0.26%, n=9). RSD of precision test was 0.070% (n=5). The average content of vincristine in liposomes was 0.790    mg/ml (RSD=0.15%, n=3), and the average entrapment efficiency was 85.94% (RSD=2.08%, n=3). CONCLUSIONS: The method is accurate, reliable, simple and rapid, and can be used for the determination of the content and entrapment efficiency of vincristine in transferrin modified vincristine-tetrandrine liposomes.
期刊: 2016年第27卷第22期
作者: 李学涛,唐炜,姜英,程岚
AUTHORS: LI Xuetao,TANG Wei,JIANG Ying,CHENG Lan
关键字: 长春新碱;粉防己碱;转铁蛋白;脂质体;含量测定;包封率;高效液相色谱法
KEYWORDS: Vincristine; Tetrandrine; Transferrin; Liposomes; Content determination; Entrapment efficiency; HPLC
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