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五味子抗氧化活性组分筛
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| 篇名： | 五味子抗氧化活性组分筛 |
| TITLE： | |
| 摘要： | 目的：筛选五味子抗氧化的活性组分。方法：以1，1-二苯基-2-三硝基苯肼（DPPH）自由基清除能力（半数清除浓度，IC50）为指标，以乙醇体积分数、料液比及提取时间为考察因素，设计正交试验优化五味子的提取工艺并进行验证；采用大孔树脂吸附法对五味子提取物进行分离纯化，以水和10％、30％、50％、70％、95％乙醇梯度洗脱，分别得到组分SC-0、SC-10、SC-30、SC-50、SC-70、SC-95，以IC50和总抗氧化能力（ABTS法测定）为考察指标（以维生素C为阳性对照），优选五味子抗氧化活性组分；采用高效液相色谱法对五味子各组分的5种木脂素含量进行测定。结果：五味子的最优提取工艺为60％乙醇、料液比1 ∶ 14、提取2.0 h；验证试验中IC50平均值为23.81 mg/ml（RSD＝0.52％，n＝3）；SC-0无抗氧化活性，其余各组分IC50值由低到高（即清除能力大小）为阳性对照＞SC-50＞SC-30＞SC-95＞SC-70＞SC-10；总抗氧化能力大小为SC-50＞阳性对照＞SC-30＞SC-70＞SC-95＞SC-10 ；各组分中5种木脂素总含量大小为SC-70＞SC-50＞SC-95＞SC-30。结论：50％乙醇洗脱物为五味子抗氧化的活性组分。 |
| ABSTRACT： | OBJECTIVE： To screen antioxidant active components of Schisandra chinensis. METHODS： The orthogonal test was adopted to optimize extraction technology using DPPH free radical scavenging activity （IC50） as index and ethanol volume fraction， material-liquid ratio and extraction time as factors， and the verification test were made. The fractions （SC-0， SC-10， SC-30， SC-50， SC-70， SC-95） were made by extracting and purifying S. chinensis with macroporous resin with water and 10％， 30％， 50％， 70％ and 95％ ethanol. With IC50 and total antioxidant capacity （determined by ABTS method） as indexes （vitamin C as positive control）， the antioxidant active components of S. chinensis were optimized. The contents of 5 kinds of lignan in different positions of S. chinensis were determined by HPLC. RESULTS： The optimal extraction condition of S. chinensis was as follows as 60％ ethanol， material-liquid ratio of 1 ∶ 14， extracting for 2.0 h. The average IC50 of DPPH free radical scavenging activity was 23.81 mg/ml （RSD＝0.52％， n＝3） in verification test. SC-0 did not have antioxidant abilities. DPPH free radical scavenging activity of those components （ie. the IC50 value from low to high） were in the following order of positive control＞SC-50＞SC-30＞SC-95＞SC-70＞SC-10； total antioxidant ability of them were in the following order of SC-50＞positive control＞SC-30＞SC-70＞SC-95＞SC-10； the contents of 5 types of lignan in different components were in the following order of SC-70＞SC-50＞SC-95＞SC-30. CONCLUSIONS： The antioxidant active component of S. chinensis is 50％ ethanol eluate. |
| 期刊： | 2016年第27卷第19期 |
| 作者： | 金银萍，侯微，高薇，刘俊霞，王玉帅，焉石，王英平 |
| AUTHORS： | JIN Yinping，HOU Wei，GAO Wei，LIU Junxia，WANG Yushuai，YAN Shi，WANG Yingping |
| 关键字： | 五味子；抗氧化；活性组分；提取工艺；正交试验；洗脱物；DPPH自由基清除能力；总抗氧化能力 |
| KEYWORDS： | Schisandra chinensis； Antioxidation； Active components； Extraction technology； Orthogonal test； Eluate； DPPH free radical scavenging activity； Total antioxidant ability |
| 阅读数： | 314 次 |
| 本月下载数： | 2 次 |
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