RP-HPLC法同时测定蒙药蓝盆花中木犀草素和芹菜素的含量
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篇名: RP-HPLC法同时测定蒙药蓝盆花中木犀草素和芹菜素的含量
TITLE:
摘要: 目的:建立同时测定蒙药蓝盆花中木犀草素和芹菜素含量的方法。方法: 采用反相高效液相色谱法。色谱柱为Diamond C18,流动相为乙腈-0.4%磷酸(34 ∶ 66,V/V),流速为1.0 ml/min,检测波长为350nm,柱温为30 ℃,进样量为10 μl。结果:木犀草素和芹菜素的检测进样量线性范围分别为66~396 ng(r=0.999 8)、93~558 ng(r=0.999 6);精密度、稳定性、重复性试验的RSD<2%;加样回收率分别为 98.15%~101.79%(RSD=1.42%,n=6)、98.66%~104.05%(RSD=1.81%,n=6)。结论:该方法操作简便,精密度、稳定性、重复性好,可用于同时测定蒙药蓝盆花中木犀草素和芹菜素的含量。
ABSTRACT: OBJECTIVE: To establish a method for simultaneous determination of luteolin and apigenin in Mongolian medicine Scabiosa atropurea. METHODS: HPLC was performed on the column of Diamond C18 with mobile phase of acetonitrile-0.4% phosphoric acid (34 ∶ 66,V/V) at a flow rate of 1.0 ml/min,the detection wavelength was 350 nm, column temperature was 30 ℃, and the injection volume was 10 μl. RESULTS: The linear range was 66-396 ng for luteolin (r=0.999 8) and 93-558 ng for apigenin(r=0.999 6); RSDs of precision, stability and reproducibility tests were lower than 2%; recoveries were 98.15%-101.79% (RSD=1.42%,n=6) and 98.66%-104.05%(RSD=1.81%,n=6),respectively. CONCLUSIONS: The method is simple with good precise, stability and reproducibility, and can be used for the simultaneous determination of luteolin and apigenin in Mongolian medicine S. atropurea.
期刊: 2016年第27卷第18期
作者: 王美丽,田香,白玉霞
AUTHORS: WANG Meili,TIAN Xiang,BAI Yuxia
关键字: 蒙药蓝盆花;木犀草素;芹菜素;反相高效液相色谱法
KEYWORDS: Mongolian medicine Scabiosa atropurea; Luteolin; Apigenin; RP-HPLC
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