ICP-MS/ICP-OES法结合化学计量学分析法比较不同产地牡丹皮药材中无机元素的含量
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篇名: ICP-MS/ICP-OES法结合化学计量学分析法比较不同产地牡丹皮药材中无机元素的含量
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摘要: 目的:比较不同产地15批牡丹皮药材中无机元素的含量,为牡丹皮道地药材的鉴别提供依据。方法:采用电感耦合等离子体质谱(ICP-MS)/电感耦合等离子体发射光谱(ICP-OES)法测定不同产地15批牡丹皮药材中无机元素的含量。ICP-MS条件:功率为1 200 W,雾化气压力为1.6 bar,分子泵电机电流为2 A,分子泵转速为1 000 Hz,炬箱调谐参数为Load 59、Tune 190。ICP-OES条件:功率为1 300 W,等离子柜气流量为15 L/min,辅助冷却气流量为0.2 L/min,雾化器流量为0.8 L/min,读数时间(自动)为1~5 s,进样量为1.5 ml/min。采用SPSS 19.0统计软件对15批牡丹皮药材进行聚类分析;采用SIMCA-P 11.0 软件联合主成分分析(PCA)法、偏最小二乘聚类判别模式分析(PLS-DA)法对15批牡丹皮药材进行主要元素分析。结果:聚类分析显示道地产区(铜陵)与非道地产区可较好分为两类; PCA法可将不同产地牡丹皮药材分别聚成不同组别;PLS-DA法显示牡丹皮药材中主要无机元素为锂、锌、铅、铁、钾。结论:牡丹皮道地药材与非道地药材在无机元素的含量与种类上存在一定的差异。
ABSTRACT: OBJECTIVE:To compare and analyze the contents of inorganic elements in 15 kinds of Paeonol saffuruticosa from different origins,and to lay the fowndation for the discrimination of its genuine regional drug. METHODS:ICP-MS and ICP-OES were conducted to determine the contents of inorganic elements in 15 kinds of P. saffuruticosa. ICP-MS determination conditions: the powder was 1 200 W, atomizer pressure was 1.6 bar, molecular pump motor current was 2 A, molecular pump speed was 1 000 Hz, bake box tuning parameters were Load 59 and Tune 190; ICP-OES determination conditions: the powder was 1 300 W, plasma gas flow rate was 15 L/min, auxiliary cooling gas flow was 0.2 L/min, atomizer flow rate was 0.8 L/min, reading time (automatic) was 1-5 s, and the injection volume was 1.5 ml/min. SPSS 19.0 software was conducted for the cluster analysis of 15 batches of samples; SIMCA-P 11.0 software was combined with principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) to analyze the main elements in 15 batches of samples. RESULTS: Cluster analysis showed specific areas (Tongling) and non-specific area may be better and divided into two types; PCA can cluster the medicines from different origins into different groups; PLS-DA shows the main elements in samples were lithium, zinc, lead, iron and potassium. CONCLUSIONS: The contents of inorganic elements in P. saffuruticosa genuine regional drug and non-genuine regional drug one show certain differences with the types.
期刊: 2016年第27卷第9期
作者: 蒋磊,胡云飞,裴月梅,徐国兵,吴德玲,徐倩
AUTHORS: JIANG Lei,HU Yunfei,PEI Yuemei,XU Guobing,WU Deling,XU Qian
关键字: 牡丹皮;电感耦合等离子体质谱;电感耦合等离子体发射光谱;无机元素;主成分分析;偏最小二乘法-判别模式分析
KEYWORDS: Paeonol saffuruticosa; ICP-MS; ICP-OES; Inorganic elements; Principal component analysis; PLS-DA
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