HPLC 法同时测定淡竹叶中绿原酸和牡荆素的含量
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篇名: HPLC 法同时测定淡竹叶中绿原酸和牡荆素的含量
TITLE:
摘要: 目的:建立同时测定淡竹叶中绿原酸和牡荆素含量的方法。方法:采用高效液相色谱法。色谱柱为Waters Atlantis C18,流动相为乙腈-水(梯度洗脱),流速为1.0 ml/min,检测波长为280 nm,柱温为35 ℃,进样量为10 μl。结果:绿原酸和牡荆素检测进样量线性范围分别为0.041 0~1.228 8(r=0.999 8)、0.264 0~7.920 0 μg(r=0.999 9);精密度、稳定性、重复性试验的RSD<2%;加样回收率分别为97.6%~102.3%(RSD=1.85%,n=9)、97.6%~101.3%(RSD=1.19%,n=9)。结论:该方法操作简便、稳定、重复性好,可用于淡竹叶中绿原酸和牡荆素含量的同时测定。
ABSTRACT: OBJECTIVE:To establish a method for the simultaneous determination of chlorogenic acid and vitexin in Lophatherum gracile. METHODS: HPLC was performed on the column was Waters Atlantis C18 with mobile phases of acetonitrile - water (gradient elution) at a flow rate of 1.0 ml/min, detection wavelength was 280 nm, column temperature was 35 ℃, and the injection volume was 10 μl. RESULTS:The linear range was 0.041 0-1.228 8 μg for chlorogenic acid (r=0.999 8) and 0.264 0-7.920 0 μg for vitexin(r=0.999 9); RSDs of precision, stability and reproducibility tests were lower than 2%;recoveries were 97.6%-102.3% (RSD=1.85%, n=9) and 97.1%-101.3%(RSD=1.19%,n=9), respectively. CONCLUSIONS: The method is simple, stable and reproducible, and can be used for the simultaneous determination of chlorogenic acid and vitexin in L. gracile.
期刊: 2016年第27卷第6期
作者: 时海燕,徐男,王玉团,苏乐群,李宏建
AUTHORS: SHI Haiyan,XU Nan,WANG Yutuan,SU Lequn,LI Hongjian
关键字: 淡竹叶;绿原酸;牡荆素;含量测定
KEYWORDS: Lophatherum gracile; Chlorogenic acid; Vitexin; Content determination
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