加味麻黄蝉衣汤对变应性鼻炎模型豚鼠的改善作用及其机制
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篇名: 加味麻黄蝉衣汤对变应性鼻炎模型豚鼠的改善作用及其机制
TITLE:
摘要:

目的:研究加味麻黄蝉衣汤对变应性鼻炎(AR)模型豚鼠的改善作用及其机制。方法:将32 只豚鼠随机均分为正常

(0.9%氯化钠注射液)组、模型(0.9%氯化钠注射液)组、实验[加味麻黄蝉衣汤,5.18 g(生药)/kg]组和对照[丙酸氟替卡松鼻喷雾

剂,50 μg/(只·d)]组,除正常组外其余各组豚鼠ip 卵清蛋白和氢氧化铝各30 mg复制AR模型。除对照组喷雾给药外,其余各组ig

相应药物,每天2 次,连续15 d。观察豚鼠的鼻部症状并进行评分,免疫组化法检测鼻黏膜酪氨酸磷酸酶1(SHP-1)的蛋白表达水

平,酶联免疫吸附法检测血清中白细胞介素4(IL-4)、干扰素γ(IFN-γ)的含量。结果:与正常组比较,模型组豚鼠出现AR症状,评

分增加,鼻黏膜SHP-1 蛋白表达水平升高,血清中IL-4 含量增加、IFN-γ含量减少(P<0.05);与模型组比较,实验组和对照组豚鼠

AR症状改善,评分降低,鼻黏膜SHP-1 蛋白表达水平降低,血清中IL-4 含量减少、IFN-γ含量增加(P<0.05)。结论:加味麻黄蝉衣

汤对AR有一定的改善作用,其机制可能与减少血清中IL-4 的含量、增加血清中IFN-γ的含量有关。

ABSTRACT:

OBJECTIVE:To study improvement effect of modified mahuang chanyi decoction on allergic rhinitis(AR)model

guinea pigs and its mechanism. METHODS:32 guinea pigs were randomly divided into normal group (0.9% Sodium chloride injection),

model group(0.9% Sodium chloride injection),experimental [Modified mahuang chanyi decoction,5.18 g(medicinal

materials)/kg] group and control [Fluticasone propionate nasal spray,50 μg/(guinea pig·d)] group. Except for normal group,other

groups received ovalbumin 30 mg and calmogastrin 30 mg intraperitoneally to induce AR model. Except for control group,other

groups were given relevant medicine intragastrically,twice a day for consecutive 15 d. Nasal symptoms of guinea pigs were observed

and recorded,and then analyzed and scored. The protein expression of nasal mucosa SHP-1 was detected by immunohistochemistry,

the serum contents of IL-4 and IFN-γ were determined by enzyme-linked immunosorbent assay. RESULTS:Compared

with normal group,model group suffered from AR symptom,and nasal symptoms score was increased;and the protein expression

of nasal mucosa SHP-1 and the serum content of IL-4 increased,while the content of IFN-γ decreased(P<0.05). Compared with

model group,AR symptom of experimental group and control group were improved;nasal symptoms score,the protein expression

of nasal mucosa SHP-1 and the serum content of IL-4 decreased while the content of IFN-γ increased(P<0.05). CONCLUSIONS:

Modified mahuang chanyi decoction shows certain improvement effect on AR,and its mechanism is associated the decrease of IL-4

content and the increase of IFN-γ content in serum.

期刊: 2016年第27卷第4期
作者: 廖勇,龚成,谢琳玲,杨雪,黄卉
AUTHORS: LIAO Yong,GONG Cheng,XIE Linling,YANG Xue,HUANG Hui
关键字: 加味麻黄蝉衣汤;变应性鼻炎;白细胞介素4;干扰素γ;豚鼠
KEYWORDS: Modified mahuang chanyi decoction;Allergic rhinitis;IL-4;IFN-γ;Guinea pig
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