目的：建立微量血浆中酒石酸唑吡坦的含量测定方法。方法：取大鼠，ig 酒石酸唑吡坦溶液3 mg/kg，给药后取血0.2 ml，

分离后取血浆50 μl 经甲醇沉淀蛋白，取上清液采用高效液相色谱-荧光法测定，以外标法进行定量。色谱柱为Agilent HC-C18，流

动相为0.03 mol/L 磷酸二氢钾溶液（含0.2％三乙胺）-甲醇（33 ∶67，V/V），流速为1.0 ml/min，激发波长为254 nm，发射波长为390

nm，进样量为20 μl。结果：酒石酸唑吡坦检测质量浓度的线性范围为2～200 μg/L（r＝0.999 7），定量下限为2 μg/L；方法回收率为

（96.96±1.35）％～（105.0±5.36）％（RSD 为2.20％～4.88％，n＝5）；提取回收率为（79.72±0.01）％～（80.77±0.02）％（RSD 为

1.34％～3.90％，n＝5）；日内RSD为1.40％～5.10％，日间RSD为3.22％～9.25％（n＝5）。结论：本法简便、灵敏，可用于微量血浆

中唑吡坦的含量测定。

OBJECTIVE：To establish a method for the content determination of zolpidem tartrate in microsamples of rat plasma.

METHODS：Rats were given zolpidem tartrate solution 3 mg/kg intragastrically，and 0.2 ml blood sample were collected and

isolated. 50 μl plasma was precipitated by methanol，and the supernatant was determined by HPLC-fluorescence combined with external

method. Agilent HC-C18 column was used with mobile phase consisted of 0.03 mol/L KH2PO4 solution（containing 0.2％ triethylamine）-

methanol（33 ∶ 67，V/V）at flow rate of 1.0 ml/min. The excitation and emission wavelengths were 254 nm and 390

nm，respectively. The sample size was 20 μ l. RESULTS：The linear ranges of zolpidem tartrate in plasma was 2-200 μg/L（r＝

0.999 7），and the limit of quantification was 2 μg/L. The method recoveries of zolpidem were（96.96±1.35）％-（105.0±5.36）％

（RSD＝2.20％-4.88％，n＝5），and extraction recoveries were（79.72±0.01）％-（80.77±0.02）％（RSD＝1.34％-3.90％，n＝5）.

The intra-day and inter-day RSDs were 1.40％-5.10％ and 3.22％-9.25％（n＝5），respectively. CONCLUSIONS：The method is

simple，sensitive and suitable for the content determination of zolpidem tartrate in microsamples of plasma.