复方鱼鹅滴鼻剂对过敏性鼻炎大鼠的干预作用及机制
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篇名: 复方鱼鹅滴鼻剂对过敏性鼻炎大鼠的干预作用及机制
TITLE: Intervention effects and mechanism of Compound yu ’e nasal drops on allergic rhinitis in rats
摘要: 目的 研究复方鱼鹅滴鼻剂对卵清蛋白致大鼠过敏性鼻炎的干预作用及机制。方法采用卵清蛋白复制大鼠过敏性鼻炎模型。造模成功的大鼠随机分为模型组、曲安奈德组(阳性对照,0.026mg/kg)和复方鱼鹅滴鼻剂高、中、低剂量组(134.4、67.2、33.6mg/kg),另设空白对照组,每组12只。除空白对照组外,各组滴鼻给予相应药物,每日2次,连续给药14d。末次给药1h后,记录大鼠鼻部症状评分;采用酶联免疫吸附测定法检测大鼠血清中免疫球蛋白E(IgE)、白细胞介素2(IL-2)、IL-13、肿瘤坏死因子α(TNF-α)水平;采用HE染色法观察大鼠鼻黏膜组织病理情况;采用蛋白质印迹法检测大鼠鼻黏膜组织IL-2、IL-13、TNF-α蛋白的表达水平。结果与空白对照组比较,模型组大鼠鼻部症状评分和血清中IgE、IL-2、IL-13、TNF-α水平均显著升高(P<0.01),鼻黏膜组织病理损伤明显,鼻黏膜组织中TNF-α、IL-2、IL-13蛋白表达水平均显著升高(P<0.01)。与模型组比较,复方鱼鹅滴鼻剂能不同程度地降低模型大鼠鼻部症状评分和血清中IgE、IL-2、IL-13、TNF-α水平,改善模型大鼠鼻黏膜组织病理损伤,抑制鼻黏膜组织中TNF-α、IL-2、IL-13的蛋白表达(P<0.05或P<0.01)。结论复方鱼鹅滴鼻剂具有明显的抗大鼠过敏性鼻炎作用,该作用可能是通过调节1型辅助性T细胞/2型辅助性T细胞平衡、抑制炎症细胞因子分泌等来实现的。
ABSTRACT: OBJECTIVE To study the intervention effects and mechanism of Compound yu ’e nasal drops on ovalbumin induced allergic rhinitis in rats . METHODS The allergic rhinitis model of rat was induced with ovalbumin . Model rats were randomly divided into model group ,triamcinolone acetonide group (positive control ,0.026 mg/kg),Compound yu ’e nasal drops high-dose,medium-dose and low -dose groups (134.4、67.2、33.6 mg/kg),12 rats in each group . Another blank control group was set. Except for blank control group ,the corresponding drugs were given by nasal drip twice a day for 14 days. One hour after last administration,the nasal symptom scores of rats were recorded ;the levels of serum immunoglobulin E (IgE),interleukin-2(IL- 2),IL-13 and tumor necrosis factor -α(TNF-α)were measured by enzyme -linked immunosorbent assay . The changes of nasal mucosa in rat were observed by HE staining . The expressions of TNF -α,IL-2 and IL -13 in nasal mucosa were detected by Western blot. RESULTS Compared with blank control group ,nasal symptom score and the levels of serum IgE ,IL-2,IL-13,TNF-α in model group were increased significantly (P<0.01);obvious pathological injury was found in nasal mucosa ,and the expressions of TNF -α,IL-2 and IL -13 protein were increased significantly (P<0.01). Compared with model group ,Compound yu ’e nasal drops significantly reduced the nasal symptom score ,the levels of serum IgE ,IL-2,IL-13,TNF-α to different extents ,improved pathological injury of nasal mucosa and significantly inhibited the expressions of TNF -α,IL-2 and IL -13 protein(P<0.05 or P< 0.01). CONCLUSIONS Compound yu ’e nasal drops play significant effects against allergic rhinitis in rats by regulating the balance of t ype 1 helper T cells/type 2 helper T cells ,balancing and inhibiting the secretion of inflammatory cytokines .
期刊: 2022年第33卷第18期
作者: 屈相玲,骆红梅,熊成欢,刘明,冯果
AUTHORS: QU Xiangling,LUO Hongmei,XIONG Chenghuan,LIU Ming,FENG Guo
关键字: 复方鱼鹅滴鼻剂;过敏性鼻炎;1型辅助性T细胞/2型辅助性T细胞平衡
KEYWORDS: Compound yu ’e nasal drops ;allergic rhinitis ;type 1 helper T cells/type 2 helper T cells balance
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