铁皮石斛叶中黄酮碳苷类成分分离、鉴定及抑制α-葡萄糖苷酶活性研究
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篇名: 铁皮石斛叶中黄酮碳苷类成分分离、鉴定及抑制α-葡萄糖苷酶活性研究
TITLE: Study on the separation and identification of flavone C-glycosides from the leaves of Dendrobium officinale and their inhibitory activities to α-gulcosidase
摘要: 目的 分离、鉴定铁皮石斛叶中的黄酮碳苷类成分,并评价其体外抑制α-葡萄糖苷酶的活性。方法采用大孔吸附树脂和制备型高效液相色谱法对铁皮石斛叶中的黄酮碳苷类成分进行分离和纯化,通过紫外光谱、核磁共振、高分辨电喷雾离子化质谱等波谱方法对化合物进行推导和鉴定。考察黄酮碳苷类成分与阳性对照(阿卡波糖)的α-葡萄糖苷酶体外抑制活性。结果从铁皮石斛叶中分离得到5个芹菜素-6,8-C-二糖苷,鉴定为芹菜素-6-C-α-L-鼠李糖-8-C-β-D-异鼠李糖(1)、夏佛塔苷(2)、异夏佛塔苷(3)、异佛莱心苷(4)和佛莱心苷(5)。化合物1~5和阿卡波糖抑制α-葡萄糖苷酶的半数抑制浓度分别为(1.79±1.27)、(2.05±0.72)、(1.93±0.67)、(1.09±0.46)、(1.36±0.58)、(18.69±1.24)μmol/L。结论从铁皮石斛叶中分离得到5个具有α-葡萄糖苷酶抑制活性的芹菜素-6,8-C-二糖苷类成分,其中化合物1为新化合物,化合物2首次从该植物中分离得到。
ABSTRACT: OBJECTIVE To separ ate and identify the flavone C-glycosides from the leaves of Dendrobium officinale ,and to evaluate their in vitro inhibitory activities to α-glucosidase. METHODS The flavone C-glycosides from the leaves of D. officinale were separated and purified by macroporous adsorption resin and preparative high -performance liquid chromatography . The structure of obtained compound was elucidated and identified by spectroscopic methods ,such as ultraviolet spectrum ,nuclear magnetic resonance,high-resolution electrospray ionization mass spectrometry ,etc. The in vitro inhibitory activities of flavone C-glycosides and positive control (acarbose)to α-glucosidase were investigated . RESULTS Five apigenin -6,8-di-C-glycosides were isolated and purified from the leaves of D. officinale,and identified as apigenin -6-C-α-L-rhamnosyl-8-C-β-D-quinovoside(1), schaftoside(2),isoschaftoside(3),isoviolanthin(4)and violanthin (5). Half inhibitory concentration of compound 1-5 and acarbose inhibiting α-glucosidase were (1.79±1.27),(2.05±0.72),(1.93±0.67),(1.09±0.46),(1.36±0.58),(18.69±1.24)μmol/L, respectively. CONCLUSIONS Five apigenin -6,8-di-C-glycosides with α-glucosidase inhibitory activity are isolated from the leaves of D. officinale,of which compound 1 is a new compound and compound 2 is isolated from this plant for the first time .
期刊: 2022年第33卷第18期
作者: 冯海林,彭崇胜,李晓波
AUTHORS: FENG Hailin,PENG Chongsheng,LI Xiaobo
关键字: 铁皮石斛叶;黄酮碳苷类;芹菜素-6-C-α-L-鼠李糖-8-C-β-D-异鼠李糖;α-葡萄糖苷酶
KEYWORDS: the leaves of Dendrobium officinale ;flavone C-glycoside;apigenin-6-C-α-L-rhamnosyl-8-C-β-D-quinovoside;α-
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