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五味子配伍对补骨脂致肝细胞氧化损伤和内质网应激的影响
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| 篇名： | 五味子配伍对补骨脂致肝细胞氧化损伤和内质网应激的影响 |
| TITLE： | Effects of compatibility of Schisandra chinensis on Psoralea corylifolia -induced oxidative damage and endoplasmic reticulum stress in hepatocytes |
| 摘要： | 目的 研究五味子配伍对补骨脂致L02肝细胞氧化损伤和内质网应激的影响，为补骨脂-五味子配伍减毒的临床应用提供参考。方法将L02细胞分为空白对照组、补骨脂模型组（1200μg/mL补骨脂，以生药量计）和补骨脂-五味子低、中、高剂量组（1200μg/mL补骨脂分别联用600、1200、2400μg/mL五味子，以生药量计）。各组细胞加培养液或药液培养48h后，检测细胞中天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）水平，检测细胞培养液中谷胱甘肽（GSH）、超氧化物歧化酶（SOD）、丙二醛（MDA）水平，检测细胞中活性氧（ROS）水平及细胞线粒体膜电位，检测细胞中葡萄糖调节蛋白78（GRP-78）、蛋白激酶R样内质网激酶（PERK）mRNA及其蛋白表达水平。结果与空白对照组比较，补骨脂模型组细胞（或其培养液）中AST、ALT、MDA、ROS水平和GRP-78、PERKmRNA及其蛋白表达水平均显著升高（P＜0.05或P＜0.01），GSH、SOD水平和线粒体膜电位均显著降低（P＜0.05或P＜0.01）；与补骨脂模型组比较，补骨脂-五味子低、中、高剂量组上述指标均显著改善（P＜0.05或P＜0.01）。结论五味子与补骨脂配伍后可以缓解补骨脂引起的L02细胞氧化损伤和内质网应激。 |
| ABSTRACT： | OBJECTIVE To study the effects of the compatibility of Schisandra chinensis on Psoralea corylifolia -induced oxidative damage and endoplasmic reticulum stress in L 02 hepatocytes，and to provide reference for clinical use of the compatibility attenuation of P. corylifolia -S. chinensis . METHODS L02 cells were divided into blank control group ，P. corylifolia model group （1 200 μg/mL P. corylifolia ，calculated by crude drug ），P. corylifolia -S. chinensis low-dose，medium-dose and high-dose groups （1 200 μg/mL P. corylifolia combined with 600，1 200，2 400 μg/mL S. chinensis ，respectively，calculated by crude drug ）. After the cells in each group were cultured in culture medium or drug solution for 48 hours，the levels of aspartate aminotransferase（AST）and alanine aminotransferase （ALT）were detected ；the levels of glutathione （GSH），superoxide dismutase （SOD）and malondialdehyde （MDA）in cell culture medium were detected ；reactive oxygen species （ROS）level and mitochondrial membrane potential in cells were detected ；mRNA and protein expressions of glucose-regulated protein 78（GRP-78）and protein kinase R-like endoplasmic reticulum kinase （PERK）were detected. RESULTS Compared with blank control group ，the levels of AST，ALT，MDA and ROS ，mRNA and protein expressions of GRP- 78 and PERK were increased significantly in P. corylifolia model group （P＜0.05 or P＜0.01）；while GSH and SOD levels ，mitochondrial membrane potential were decreased significantly （P＜0.05 or P＜0.01）. Compared with P. corylifolia model group ，above indexes of P. corylifolia -S. chinensis low-dose， medium-dose and high-dose groups were all improved significantly （P＜0.05 or P＜0.01）. CONCLUSIONS The compatibility of P. corylifolia -S. chinensis can alleviate P. corylifolia -induced oxidative damage and endoplasmic reticulum stress of L 02 cells. |
| 期刊： | 2022年第33卷第09期 |
| 作者： | 张婧茜，殷佳，曲晓琳，刘慧，王娜，闫佳靓，傅春升 |
| AUTHORS： | ZHANG Jingxi，YIN Jia，QU Xiaolin ，LIU Hui，WANG Na，YAN Jiajing ，FU Chunsheng |
| 关键字： | 五味子；补骨脂；配伍减毒；肝损伤；氧化损伤；内质网应激 |
| KEYWORDS： | Schisandra chinensis ；Psoralea corylifolia ；compatibility attenuation ；liver injury ；oxidative damage ；endoplasmic |
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