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芍药甘草汤抗胃溃疡作用的谱效关系
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| 篇名： | 芍药甘草汤抗胃溃疡作用的谱效关系 |
| TITLE： | Spectrum-effect relationship of anti-gastric ulcer effect of Shaoyao gancao decoction |
| 摘要： | 目的 研究芍药甘草汤抗胃溃疡作用的谱效关系。方法制备11批芍药甘草汤，以无水乙醇造模法制备胃溃疡大鼠模型，以胃康灵为阳性对照，考察各批芍药甘草汤对模型大鼠胃组织中防御因子一氧化氮（NO）、表皮生长因子（EGF）、超氧化物歧化酶（SOD）和攻击因子丙二醛（MDA）含量的影响。采用《中药色谱指纹图谱相似度评价系统（2004A版）》建立11批芍药甘草汤的HPLC指纹图谱并进行相似度评价，通过与混合对照品比对指认共有峰。通过灰色关联分析法分析芍药甘草汤抗胃溃疡作用的谱效关系。结果11批芍药甘草汤均可显著降低胃溃疡模型大鼠胃组织中MDA的含量，显著增加NO、EGF、SOD的含量（P＜0.01），对胃溃疡有一定的改善作用。11批样品的图谱中共有23个共有峰，与对照指纹图谱的相似度均大于0.9；通过与混合对照品比对，共指认了7个共有峰，分别为没食子酸（峰5）、芍药内酯苷（峰9）、芍药苷（峰10）、甘草苷（峰12）、芹糖异甘草苷（峰14）、异甘草苷（峰15）、甘草酸（峰22）。7个指认共有峰和药效指标的平均关联度均大于0.6，其中又以峰22（甘草酸）、峰10（芍药苷）、峰12（甘草苷）的平均关联度较大，分别为0.807、0.772、0.770。结论芍药甘草汤抗胃溃疡作用是多个成分协同作用的结果，其中甘草酸、芍药苷、甘草苷可能是其主要的药效贡献成分。 |
| ABSTRACT： | OBJECTIVE To study the sp ectrum-effect relationship of anti-gastric ulcer effect of Shaoyao gancao decoction. METHODS Eleven batches of Shaoyao gancao decoction were prepared ；gastric ulcer model was established by anhydrous ethanol modeling method. Using Weikangling as positive control ，the effects of Shaoyao gancao decoction on the contents of defensive factors [nitric oxide （NO），epidermal growth factor （EGF），superoxide dismutase （SOD）] and attack factor [malondialdehyde （MDA）] in gastric tissue of model rats were investigated. HPLC fingerprints of 11 batches of Shaoyao gancao decoction were established and similarity evaluation was performed with Similarity Evaluation System of Traditional Chinese Medicine Chromatographic Fingerprint （2004A edition ）； common peaks were identified by comparing with mixed control. The spectrum-effect relationship of Shaoyao gancao decoction against gastric ulcer was analyzed based on the grey correlation analysis. RESULTS Eleven batches of Shaoyao ganyao decoction could significantly decrease the content of MDA in gastric tissue ，while increased the contents of NO ，EGF and SOD in gastric ulcer model rats （P＜0.01），and had a certain inhibitory effect on the gastric ulcer. There were 23 common peaks in chromatograms of 11 batches of samples ，and the similarity with the control fingerprint was not less than 0.9. By comparing with mixed control ，7 common peaks were identified ，namely gallic acid （peak 5），albiflorin（peak 9），paeoniflorin（peak 10），liquiritin（peak 12），isoliquiritin apioside （peak 14），isoliquiritoside（peak 15）， glycyrrhizic acid （peak 22）. The average correlation degree of 7 identified common peaks and pharmacodynamic indexes were greater than 0.6，of which peak 22（glycyrrhizic acid ），peak 10（paeoniflorin）and peak 12（liquiritin）had the largest correlation ， and their values were 0.807，0.772 and 0.770 respectively. RESULTS The anti-gastric ulcer effect of Shaoyao gancao decoction is the result of the synergistic effect of multiple components ，among which glycyrrhizic acid ，paeoniflorin and liquiritin may be the main pharmacodynamic components. |
| 期刊： | 2022年第33卷第06期 |
| 作者： | 何利，付志丽，熊燕，赵坤，杨滔，杨玲，刘文 |
| AUTHORS： | HE Li，FU Zhili，XIONG Yan，ZHAO Kun，YANG Tao，YANG Ling，LIU Wen |
| 关键字： | 芍药甘草汤；抗胃溃疡；谱效关系；灰色关联分析 |
| KEYWORDS： | Shaoyao gancao decoction ；anti-gastric ulcer ；spectrum-effect relationship ；grey correlation analy sis |
| 阅读数： | 180 次 |
| 本月下载数： | 1 次 |
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