篇名: Foretinib衍生物LWK-126在不同种属肝微粒体中的代谢稳定性研究
TITLE: Study on Metabolic Stability of Foretinib Derivative LWK- 126 in Liver Microsomes of Different Species
摘要: 目的:建立测定肝微粒体中Foretinib衍生物LWK-126质量浓度的方法,研究其在大鼠、比格犬、人肝微粒体中的代谢稳定性。方法:采用体外肝微粒体孵育体系,将LWK-126分别溶于由还原型烟酰胺腺嘌呤二核苷酸磷酸溶液启动的大鼠、比格犬、人肝微粒体孵育体系中,于37℃水浴下分别孵育0、5、10、20、30、60min时加入含内标(1μg/mL甲苯磺丁脲)的乙腈终止反应,并采用超高效液相色谱-串联质谱法检测各孵育体系中LWK-126的质量浓度。以WatersBEHC18为色谱柱,以水(含0.01%甲酸)-乙腈(含0.01%甲酸)为流动相进行梯度洗脱,流速为0.4mL/min,柱温为30℃,进样量为2μL;离子源为电喷雾电离源,采用正离子模式采集,扫描范围为m/z50→1200。以孵育0min时LWK-126的质量浓度为参照,计算其在不同孵育体系中的剩余百分率与酶动力学参数。结果:LWK-126检测质量浓度的线性范围为0.05~15μg/mL(R2=0.9992),定量下限为0.05μg/mL,最低检测限为0.01μg/mL,精密度、准确度、提取回收率和基质效应均符合生物样品定量分析的要求。LWK-126在人、大鼠、比格犬肝微粒体中孵育60min后的剩余百分率分别为(33.17±4.52)%、(3.14±6.73)%、(1.38±5.85)%,半衰期分别为33.15、11.76、5.62min,清除率分别为38.45、118.81、245.76μL(/min·mg)。结论:成功建立了肝微粒体中LWK-126质量浓度的测定方法。LWK-126在不同种属肝微粒体中的代谢稳定性排序依次为人>大鼠>比格犬。
ABSTRACT: OBJECTIVE:To establish the metho d for the concentration det ermination of foretinib derivative LWK- 126 in liver microsomes,and to study its metabolism stability in liver microsomes of rats ,Beagle dogs and human. METHODS :In the in vitro incubation system of liver microsomes ,LWK-126 was dissolved in liver microsomal incubation systems of rats ,Beagle dog and human initiated by reduced nicotinamide adenine dinucleotide phosphate solution. After incubation in water at 37 ℃ for 0,5,10,20, 30 and 60 min,the reaction was terminated with acetonitrile containing internal standard (1 μg/mL tolbutamide). UPLC-MS/MS method was applied to determine the concentration of LWK- 126 in the incubation systems. The determination was performed on Waters BEH C 18 column with mobile phase consisted of water (containing 0.1% formic acid )-acetonitrile(containing 0.1% formic acid)by gradient elution at the flow rate of 0.4 mL/min. The column temperature was 30 ℃,and the sample size was 2 μL. The mass spectral analysis was performed in a positive electrospray ionization mode ,and the full MS experiment was run with the selective reaction monitoring mode with a scanning range of m/z 50→1 200. Taking the concentration of LWK- 126 at 0 min as reference,the remaining percentage and the enzyme kinetic parameters were calculated. RESULTS :The linear range of LWK- 126 was 0.05-15 μg/mL(R 2=0.999 2);the lower limit of quantification was 0.05 μg/mL,and the lowest detection limit was 0.01 μg/mL. The precision,accuracy,extraction recovery and matrix effect all met the analysis requirements of biological samples. The remaining percentage of LWK- 126 in liver microsomes of human ,rats and Beagle dogs for 60 min were (33.17±4.52)%,(3.14± 6.73)%,(1.38±5.85)%;t1/2 of them were 33.15,11.76,5.62 min;the clearance rates were 38.45,118.81,245.76 μL(/ min·mg), respectively. CONCLUSIONS :The method for the content ; determination of LWK- 126 in liver microsomes is established successfully. The order of metabolic stability of LWK- 126 in 〔2016〕4015) liver microsomes of different species is human >rats>Beagle dogs.
期刊: 2021年第32卷第11期
作者: 王忠元,杨华丽,崔杏,朱高峰,汤磊,廖伟科
AUTHORS: WANG Zhongyuan,YANG Huali,CUI Xing,ZHU Gaofeng,TANG Lei,LIAO Weike
关键字: LWK-126;超高效液相色谱-串联质谱法;肝微粒体;代谢稳定性
KEYWORDS: LWK-126;UPLC-MS/MS;Liver microsomes ;
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