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高乌头炮制前后乙醇提取物石油醚萃取部位的GC-MS分析
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| 篇名： | 高乌头炮制前后乙醇提取物石油醚萃取部位的GC-MS分析 |
| TITLE： | GC-MS Analysis of Petroleum Ether Fraction from the Ethanol Extract of Aconitum sinomontanum before and after Processing |
| 摘要： | 目的：比较高乌头炮制前后其乙醇提取物石油醚萃取部位的化学成分。方法：取高乌头药材用水净制润软后，制得高乌头生品饮片；取生品饮片用甘草汁经高压蒸制，制得高乌头炮制品饮片；分别以95％乙醇超声提取后再经石油醚萃取，制得高乌头生品、炮制品乙醇提取物石油醚萃取部位。采用气质联用技术（GC-MS）分析两者的化学成分；采用NIST2014标准谱图库进行化合物对比、匹配；采用峰面积归一化法测定各成分的相对百分含量。结果：炮制前后，高乌头乙醇提取物石油醚萃取部位中的主要成分均为脂肪酸及其酯类。其中，高乌头生品饮片中共检测出18个色谱峰，鉴定出13个化合物，占生品挥发性成分总量的94.60％，以（Z，Z，Z）-9，12，15-十八烷三烯酸（26.13％）、十六酸乙酯（25.27％）、棕榈油酸（10.84％）、亚油酸乙酯（10.67％）、（Z，Z）-9，12-十八烷二烯酸甲酯（6.66％）、十五烷酸（5.11％）的相对百分含量较高；高乌头炮制品饮片中共检测出25个色谱峰，鉴定出18个化合物，占炮制品挥发性成分总量的82.40％，以棕榈油酸（18.95％）、（Z，Z）-9，12-十八烷二烯酸甲酯（17.93％）、十六酸乙酯（11.94％）、（Z，Z，Z）-9，12，15-十八烷三烯酸（10.54％）、（Z，Z）-9，12-十八烷二烯酸（5.51％）、（Z）-11-十六烷烯酸（5.30％）的相对百分含量较高。炮制后新增7种成分，共鉴定出其中5种，分别为（－）-蓝桉醇、2-甲基十四酸乙酯、6-甲基-4苯基香豆素、β-谷甾醇、二十七烷；炮制后未有成分消失，且部分成分含量增加或减少。结论：经炮制后，高乌头乙醇提取物的石油醚萃取部位挥发性成分存在差异，炮制后新增（－）-蓝桉醇等多种成分。 |
| ABSTRACT： | OBJECTIVE：To compare the chemical constituents of petroleum ether fraction from ethanol extract of Aconitum sinomontanum before and after processing. METHODS ：After A. sinomontanum was purified with water ，the raw product decoction pieces were prepared ；the raw decoction pieces were steamed with licorice juice under high pressure to prepare processed decoction pieces of A. sinomontanum . The petroleum ether fractions of raw product and processed product were obtained after ultrasonic extraction with 95％ ethanol. The chemical constituents in the samples were analyzed by GC-MS. NIST 2014 mass spectrometry database was used to compare and match the components . The peak area normalization method was used to determine the relative percentage content of each component. RESULTS ：Before and after processing ，fatty acids and esters were the main components in the petroleum ether fraction from ethanol extract. Totally 18 chromatographic peaks were detected in the detection pieces of raw product，and 13 compounds were identified ，accounting for 94.60％ of the total content of volatile components. The components with relatively high content were （Z，Z，Z）-9，12，15-octadecatrienoic acid （26.13％），hexadecanoic acid ethyl ester （25.27％）， palmitoleic acid （10.84％），ethyl linoleic acid （10.67％），（Z，Z）-9，12-octadecenoic acid methyl ester （6.66％），pentadecanoic acid（5.11％）and so on. Totally 25 chromatographic peaks were detected in the decoction pieces of processed products，and 18 components were identified ，accounting for 82.40％ of the total content of volatile components. The components with relatively high content were palmitoleic acid （18.95％），（Z，Z）-9，12-octadecenoic acid methyl ester （17.93％），hexadecanoic acid ethyl ester（11.94％），（Z，Z，Z）-9，12，15-octadecatrienoic acid （10.54％），（Z，Z）-9，12-octadecenoic acid （5.51％），（Z）-11-hexadecanoic acid（5.30％）and so on. After processing ，7 new components were added ，5 of which were identified as （－）-eucalyptus globulus alcohol，ethyl 2-methyltetrade-canoate，6-methyl-4-phenylcoumarin，β-sitosterol，heptadecane. After processing ，no components disappeared，and the content of some components increased or decreased. CONCLUSIONS ：After processing ，the volatile components in the petroleum ether fraction from ethanol extract of A. sinomontanum are different ，and（－）-eucalyptus globulus alcohol and other components are added after processing. |
| 期刊： | 2021年第32卷第10期 |
| 作者： | 冯银平，帖晓燕，戴海蓉，张云鹤，樊秦，李芸 |
| AUTHORS： | FENG Yinping ，TIE Xiaoyan ，DAI Hairong ，ZHANG Yunhe ，FAN Qin，LI Yun |
| 关键字： | 高乌头；炮制；乙醇提取物；石油醚萃取部位；气质联用技术；化学成分 |
| KEYWORDS： | Aconitum sinomontanum； Processing； Ethanol extract ； Petroleum extraction fraction ； GC-MS； Chemical |
| 阅读数： | 319 次 |
| 本月下载数： | 5 次 |
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