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麻黄细辛附子汤对肾阳虚外感证模型小鼠TLRs应答及Cyt-CO介导的凋亡调控的影响
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| 篇名： | 麻黄细辛附子汤对肾阳虚外感证模型小鼠TLRs应答及Cyt-CO介导的凋亡调控的影响 |
| TITLE： | Effects of Mahuang Xixin Fuzi Decoction on TLRs Response and Cyt-CO-mediated Apoptosis Regulation in Mice with Influenza Disease of Kidney-yang Deficiency |
| 摘要： | 目的：研究麻黄细辛附子汤对肾阳虚外感证模型小鼠Toll样受体（TLRs）应答及细胞色素C氧化酶（Cyt-CO）介导的凋亡调控的影响。方法：将48只雄性Balb/c小鼠随机分为正常组（n＝12）和造模组（n＝36）。造模组小鼠采用腹腔注射苯甲酸雌二醇溶液（8mg/kg）+滴鼻接种甲型H1N1流感病毒（20μL/只）的方法建立肾阳虚外感复合模型。然后将造模成功的小鼠随机分为模型组、阳性药组（磷酸奥司他韦胶囊，0.195g/kg）和麻黄细辛附子汤组（1.802g/kg，以生药总量计），每组12只。各给药组小鼠灌胃相应药液，正常组和模型组小鼠灌胃等体积生理盐水；灌胃体积均为20mL/kg，每天1次，连续给药6天。给药期间，每天测量小鼠体质量、肛温，并计算其初始体质量百分率；末次给药后，计算其脏器（脾、胸腺、肺）指数，观察其肺组织病理学变化，检测其肺组织中甲型H1N1流感病毒的病毒载量（以M基因mRNA表达水平反映）以及心脏组织中TLR3、TLR7、髓样分化因子（MyD88）、胱天蛋白酶3（Caspase-3）mRNA表达水平和Cyt-CO活性、细胞色素C（Cyt-C）含量。结果：与正常组比较，模型组小鼠的初始体质量百分率、肛温持续降低（P＜0.05）；脾指数、胸腺指数均显著降低（P＜0.05），肺指数显著升高（P＜0.05）；肺组织病变严重；肺组织中病毒载量和心脏组织中TLR3、TLR7、MyD88、Caspase-3mRNA表达水平及Cyt-C含量均显著升高（P＜0.05或P＜0.01），心脏组织中Cyt-CO活性显著降低（P＜0.01）。与模型组比较，麻黄细辛附子汤组小鼠的初始体质量百分率、肛温自给药第4天起呈上升趋势（P＜0.05或P＜0.01）；脾指数、胸腺指数均显著升高（P＜0.05），肺指数均显著降低（P＜0.05）；肺组织病理损伤明显改善；肺组织中病毒载量和心脏组织中TLR3、Caspase-3mRNA表达水平和Cyt-C含量均显著降低（P＜0.05或P＜0.01），心脏组织中Cyt-CO活性显著升高（P＜0.01）。结论：麻黄细辛附子汤对肾阳虚外感病证模型小鼠具有改善作用，这可能与其可抑制TLRs应答和Cyt-CO介导的凋亡调控途径有关。 |
| ABSTRACT： | OBJECTIVE：To study the effects of Mahuang xixin fuzi decoction on Toll-like receptors （TLRs）response and cytochrome C oxidase （Cyt-CO）-mediated apoptosis regulation in mice with influenza disease of kidney-yang deficiency. METHODS：Totally 48 male Balb/c mice were randomly divided into normal group （n＝12）and modeling group （n＝36）. The modeling group was intraperitoneally injected with estradiol benzoate solution （8 mg/kg）and intranasally injected with influenza virus H 1N1（20 μL/mice）to establish the influenza disease compound model of kidney-yang deficiency. After modeling ，the mice were randomly divided into model group ，positive drug group （Oseltamivir phosphate capsules ，0.195 g/kg），Mahuang xixin fuzi decoction group （1.802 g/kg，by crude dru g），with 12 mice in each group. Each group was given relevant medicine intragastrically，normal group and model group were given， corresponding volume of normal saline intragastrically 20 mL/kg，once a day ，for consecutive 6 days. During admi-nistration，body weight and anal temperature of mice were mail：xsy407861520@163.com measured daily ；the percentage of initial body weight was calculated. After last medication ，the organ （spleen，thymus and lung ）indexes were calculated ；the pathological changes of lung tissue were observed. The viral load of influenza A virus H 1N1 in lung tissue was detected （reflected by M gene mRNA expression）；mRNA expressions of TLR3，TLR7，myeloid differentiation factor （MyD88）and Caspase- 3 in cardiac tissue as well as the activity of Cyt-CO and the content of cytochrome C （Cyt-C）were also determined. RESULTS ：Compared with normal group，initial body weight percentage and anal temperature of the model group continued to decrease （P＜0.05）；the spleen and thymus indexes were decreased significantly （P＜0.05），while lung index was increased significantly （P＜0.05）；the lung tissue lesions were serious. Viral load in lung tissue ，mRNA expressions of TLR 3，TLR7，MyD88 and Caspase- 3 in cardiac tissue as well as the content of Cyt-C were increased significantly （P＜0.05 or P＜0.01），while the activity of Cyt-CO in cardiac tissue was significantly decreased （P＜0.01）. Compared with model group ，initial body weight percentage and anal temperature of mice in Mahuang xixin fuzi decoction group showed an increasing trend from the fourth day of administration （P＜0.05 or P＜0.01）. The spleen and thymus indexes were increased significantly （P＜0.05），while the lung index was significantly decreased （P＜0.05）；the pathological injury of lung tissue was significantly improved ；viral load in lung tissue ，mRNA expressions of TLR 3 and Caspase- 3 as well as the content of Cyt-C in cardiac tissue were decreased significantly （P＜0.05 or P＜0.01），while the activity of Cyt-CO was increased significantly in cardiac tissue （P＜0.01）. CONCLUSIONS ：Mahuang xixin fuzi decoction can improve influenza disease of kidney-yang deficiency in mice ，the effect may related to inhibit TLRs response and apoptosis regulation pathway mediated by Cyt-CO. |
| 期刊： | 2021年第32卷第06期 |
| 作者： | 邢淑雁，范珊珊，于钦辉，马青云，孙启慧，杨勇，容蓉 |
| AUTHORS： | XING Shuyan ，FAN Shanshan ，YU Qinhui ，MA Qingyun ，SUN Qihui，YANG Yong，RONG Rong |
| 关键字： | 麻黄细辛附子汤；肾阳虚外感证；细胞色素C氧化酶；细胞色素C；凋亡；Toll样受体；小鼠 |
| KEYWORDS： | Mahuang xixin fuzi decoction ；Influenza disease of kidney-yang deficiency ；Cytochrome C oxidase ；Cytochrome |
| 阅读数： | 212 次 |
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