紫草素对人结肠癌细胞HCT116自噬和凋亡的影响
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篇名: 紫草素对人结肠癌细胞HCT116自噬和凋亡的影响
TITLE: Effects of Shikonin on Autophagy and Apoptosis of Human Colon Cancer HCT 116 Cells
摘要: 目的:研究紫草素对人结肠癌细胞HCT116自噬和凋亡的影响。方法:以0(空白对照)、10、20、40μg/mL紫草素作用于HCT116细胞48h后,采用MTT法检测细胞增殖抑制率;采用流式细胞术检测细胞凋亡率;分别采用实时荧光定量-聚合酶链式反应法和Westernblotting法检测细胞中微管相关蛋白轻链3(LC3)和自噬相关蛋白Beclin-1、p62的mRNA及其蛋白表达水平。结果:与空白对照比较,10、20、40μg/mL紫草素作用48h后HCT116细胞的增殖抑制率和凋亡率均显著升高(P<0.05或P<0.01)。10、20、40μg/mL紫草素作用于HCT116细胞48h后均可不同程度地升高细胞中LC3、Beclin-1、p62mRNA及其蛋白的表达水平,除10μg/mL紫草素作用后细胞中LC3mRNA表达水平升高不显著外,其余指标水平与空白对照比较差异均有统计学意义(P<0.05或P<0.01)。结论:紫草素可诱导人结肠癌细胞HCT116发生凋亡,并激活其自噬途径。
ABSTRACT: OBJECTIVE:To study the effects of shikonin on autophagy and apoptosis of human colon cancer HCT 116 cells. METHODS:After treating HCT 116 cells for 48 h with shikonin at 0(blank control )10,20,40 μg/mL,MTT method was used to detect inhibitory rate of cell proliferation. Flow cytometry was used to detect cell apoptosis rate. RT-qPCR assay and Western blotting were respectively used to detect mRNA and protein expressions of microtubule associated protein light chain 3(LC3)and autophagy-related protein Beclin- 1 and p 62. RESULTS :Compared with blank control ,after treated with 10,20,40 μ g/mL shikonin for 48 h,proliferation inhibitory rate and apoptosis rate of HCT 116 cells were increased significantly (P<0.05 or P< 0.01). After treated with 10,20,40 μg/mL shikonin for 48 h,mRNA and protein expressions of LC 3,Beclin-1 and p 62 in HCT116 cells were increased to different extents ;except that mRNA expression of LC 3 was not increased significantly after treated with 10 μg/mL shikonin,the difference were statistically significant in other indexes ,compared with blank control (P<0.05 or P<0.01). CONCLUSIONS :Shikonin can induce the apoptosis of human colon cancer HCT 116 cells and activate its autophagy pathway.
期刊: 2021年第32卷第01期
作者: 邵鑫,蒋先虹,王瑞,蒲强红,韩彬,刘福
AUTHORS: SHAO Xin,JIANG Xianhong,WANG Rui,PU Qianghong,HAN Bin,LIU Fu
关键字: 紫草素;人结肠癌细胞HCT116;自噬;凋亡
KEYWORDS: Shikonin;Human colon cancer HCT 116 cells;Autophagy;Apoptosis
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