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基于浸出物和特征图谱的熵权TOPSIS法综合评价仙鹤草药材质量
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| 篇名： | 基于浸出物和特征图谱的熵权TOPSIS法综合评价仙鹤草药材质量 |
| TITLE： | Comprehensive Quality Evaluation of Agrimonia pilosa with Entropy Weight TOPSIS Method Based on Extract and Characteristic Spectrum |
| 摘要： | 目的：基于浸出物和特征图谱建立仙鹤草药材的整体质量评价模型，为该药材的综合质量评价和优质种源筛选提供依据。方法：参照2015年版《中国药典》（四部）通则采用不同的提取方法和溶剂条件，以浸出物含量和浸出物高效液相色谱（HPLC）特征图谱总峰面积为指标，通过加权综合评分优选仙鹤草浸出物的提取工艺。建立15批仙鹤草药材的HPLC特征图谱，并进行相似度评价和特征峰指认。采用SPSS25.0软件对不同产地仙鹤草浸出物含量、药材特征峰总峰面积进行单因素方差分析和Pear-son相关性分析，比较不同产地药材的质量优劣。以15批仙鹤草药材的特征峰峰面积和浸出物含量为指标，采用熵权逼近理想解排序法（TOPSIS）进行该药材综合质量评价。结果：优选出以50％乙醇为溶剂、热浸法提取的仙鹤草浸出物提取工艺。15批仙鹤草药材的相似度均大于0.92，并指认了4个特征成分（鞣花酸、槲皮苷、芹菜素、山柰酚）。不同产地的仙鹤草浸出物平均含量、药材特征图谱平均总峰面积均有显著差异（P＜0.05或P＜0.01），且两者呈正相关（r＝0.86，P＜0.01）。熵权TOPSIS法评价结果显示，安徽、浙江、四川、河南、江苏产仙鹤草药材质量评价中的贴近度（C）i均值依次为0.689、0.351、0.218、0.308、0.361。结论：各批样品以安徽产仙鹤草药材质量最优，江苏、浙江产药材质量次之，河南产药材质量再次之，四川产地药材质量相对较差。所建立的仙鹤草浸出物提取工艺、药材特征图谱测定方法稳定可行；用于仙鹤草药材综合质量评价的熵权TOPSIS法客观、可量化，能有效评价该药材质量。 |
| ABSTRACT： | OBJECTIVE：To establish an overall quality evaluation model for Agrimonia pilosa based on extract and characteristic spectrum，and to provide evidence for comprehensive quality evaluation of the medicinal material and screening of high-quality provenance. METHODS ：Referring to different extraction method and solvent condition stated in 2015 edition of Chinese Pharmacopoeia （part Ⅳ），using the content and total peak area of HPLC characteristic chromatogram of extract as indexes，and the extraction technology was optimized by weighted comprehensive score. HPLC characteristic spectrum of 15 batches of A. pilosa was established ，and similarity evaluation and characteristic peak identification were performed. SPSS 25.0 software was used to conduct single factor analysis and Pearson correlation analysis for the extract content and total peak area of A. pilosa from different origins. The quality of medicinal materials from different origins were compared. Entropy weight TOPSIS method was adopted to evaluate comprehensive quality of A. pilosa using the extract content and total peak area of 15 batches of A. pilosa . RESULTS ：The extraction technology of A. pilosa extract，which was extracted with hot dip plating using 50％ ethanol as solvent ，was optimized. The similarity of 15 batches of A. pilosa was higher than 0.92，and 4 characteristic components were identified（ellagic acid ，quercetin，apigenin，kaempferol）. There were significant differences in average extract content and total peak area of characteristic chromatogram of A. pilosa from different origins （P＜0.05 or P＜0.01），and there was a certain positive correlation between them （r＝0.86，P＜0.01）. Results of entropy weight TOPSIS evaluation showed that the average Ci values of A. pilosa in Anhui ，Zhejiang，Sichuan，Henan and Jiangsu provinces were 0.689，0.351，0.218，0.308 and 0.361 respectively. CONCLUSIONS：The quality of A. pilosa from Anhui was the best ，that from Zhejiang and Jiangsu was better ，that from Henan was the second ，and that from Sichuan was poor. Established extraction technology and characteristic spectrum determination method of A. pilosa are stable and feasible. The entropy weight TOPSIS model is objective and quantifiable for comprehensive quality evaluation of A. pilosa ，and can effectively evaluate its quality. |
| 期刊： | 2020年第31卷第24期 |
| 作者： | 曾昭君，洪婉敏，钟如帆，胡瑶，潘玲，干丽 |
| AUTHORS： | ZENG Zhaojun ，HONG Wanmin ，ZHONG Rufan，HU Yao，PAN Ling，GAN Li |
| 关键字： | 仙鹤草；浸出物；特征图谱；熵权TOPSIS法；质量评价 |
| KEYWORDS： | Agrimonia pilos a； Extract； Characteristic |
| 阅读数： | 210 次 |
| 本月下载数： | 5 次 |
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