黄芪地上、地下部分对免疫低下模型小鼠保护作用的比较研究
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篇名: 黄芪地上、地下部分对免疫低下模型小鼠保护作用的比较研究
TITLE: Comparison of Protective Effects between Aboveground and Underground Parts of Astragalus membranaceus on Immunosuppression Model Mice
摘要: 目的:比较黄芪地上、地下部分对免疫低下模型小鼠的保护作用差异,为黄芪资源的进一步开发利用提供参考。方法:取240只ICR小鼠平行分为4批,每批60只,雌雄各半。各批小鼠均按体质量及性别随机分为空白组、模型组和黄芪地上、地下部分低、高剂量组(均为3、6g/kg,以生药量计),每组10只。空白组和模型组小鼠均灌胃生理盐水,黄芪组小鼠灌胃相应浓度的药物,灌胃体积均10mL/kg,每日1次,连续给药30d。除空白组外,其余各组小鼠均自给药第24天起腹腔注射环磷酰胺40mg/(kg·d),连续注射3d,建立免疫低下模型。检测各组小鼠的血清免疫球蛋白(IgG、IgM、IgA)、炎症因子[一氧化氮、白细胞介素2(IL-2)、IL-6、肿瘤坏死因子α]和半数溶血值,测定小鼠体质量、胸腺指数、脾指数、吞噬指数、自然杀伤(NK)细胞活性、脾淋巴细胞增殖能力、二硝基氟苯诱导的小鼠迟发型变态反应(以左右两耳的质量差反映)和溶血空斑数。结果:与空白组比较,模型组小鼠血清中免疫球蛋白水平、体质量、胸腺指数、脾指数、吞噬指数、NK细胞活性、脾淋巴细胞增殖能力、溶血空斑数、半数溶血值均显著降低(P<0.05),炎症因子水平、左右耳片质量差显著升高(P<0.05);与模型组比较,黄芪各给药组小鼠的上述指标均显著改善,且均具有一定的剂量依赖性(P<0.05);与黄芪地下部分组比较,黄芪地上部分同剂量组的上述指标的改善更为明显(P<0.05)。结论:黄芪地上、低下部分对免疫低下模型小鼠均具有一定的保护作用,且地上部分的作用优于地下部分。
ABSTRACT: OBJECTIVE:To compare the protective effects model mice between the aboveground and underground parts of Astragalus membranaceus on immunosuppression ,and to provide reference for further utilization and development of A. membranaceus. METHODS :A total of 240 ICR mice were divided into 4 batches,60 mice in each batch ,with half male and half female. Each batch of mice were randomly divided into blank group ,model group ,A. membranaceus aboveground part and undergroud part low-dose and high-dose groups (3,6 g/kg,by crude drug )according to body weight and sex ,with 10 mice in each group. Blank group and model group were given normal saline intragastrically. A. Membranaceus groups were given corresponding concentration of drug intragastrically ,10 mL/kg,once a day ,for consecutive 30 days. Except for blank group , other groups were intraperitoneally injected with cyclophosphamide 40 mg/(kg·d)for consecutive 3 days,since 24th day of treatment,to establish immunosuppression model. The levels of serum immunoglobulin (IgG,IgM,IgA),inflammation factors [nitric oxide ,interleukin-2(IL-2),IL-6,tumor necrosis factor-α] and half hemolysis value were detected in each group. Body weight ,thymus index ,spleen index ,phagocytic index ,activity of natural killer (NK)cell,splenic lymphocyte proliferation ability,dinitrofluorobenzene-induced delayed metamorphosis reaction in mice (by weight difference between left and right ears ) and the number of hemolytic plaque were determined. RESULTS : Compared with blank group , the serum levels of immunoglobulin,body weight ,thymus index ,spleen index ,phagocytic index ,NK cell activity ,the proliferation ability of splenic lymphocyte,the number of hemdytic plaque and half hemolysis value were decreased significantly in model group (P<0.05), while inflammation factor level as well as weight difference between left and right ears were increased significantly (P<0.05). Compared with model group ,above indexes of mice in A. membranaceus groups were improved significantly ,in dose-dependent manner(P<0.05). Compared with A. membranaceus undergroud part group ,above indexes of A. membranaceus aboveground part group were improved significantly (P<0.05). CONCLUSIONS :Aboveground and underground part of A. membranaceus both have pretective effect on immunosuppression model mice ,and the effect of aboveground part of A. membranaceus is stronger than underground part of A. membranaceus .
期刊: 2020年第31卷第24期
作者: 韩宪忠,沈正泽,袁文霄,黄英如,丁家昱
AUTHORS: HAN Xianzhong,SHEN Zhengze,YUAN Wenxiao,HUANG Yingru,DING Jiayu
关键字: 黄芪;地上部分;地下部分;免疫力;药效学
KEYWORDS: Astragalus memb ranaceus;Aboveground part ;Underground part ;Immunity;Pharmacodynamics
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