紫铆花素对PC12细胞氧化应激损伤的保护作用及对线粒体功能的影响研究
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篇名: 紫铆花素对PC12细胞氧化应激损伤的保护作用及对线粒体功能的影响研究
TITLE: Study on the Protective Effects of Butein on Oxidative Stress Injury of PC 12 Cell and Its Effects on Mitochondrial Function
摘要: 目的:研究紫铆花素对PC12细胞氧化应激损伤的保护作用及对线粒体功能的影响。方法:将大鼠PC12细胞分为正常对照组、模型组、溶剂对照组(1‰二甲基亚砜)和紫铆花素高、中、低浓度组(2、1、0.5μmol/L)。后4组给予相应试剂/药物干预24h后,除正常对照组外的其余组均以100mU/mL葡萄糖氧化酶诱导氧化应激模型。培养4h后,检测细胞存活率、凋亡率和细胞内活性氧(ROS)、丙二醛(MDA)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)、三磷酸腺苷(ATP)、白细胞介素1β(IL-1β)、肿瘤坏死因子α(TNF-α)水平或活性以及线粒体膜电位(MMP)变化。结果:与正常对照组比较,模型组细胞存活率和SOD、CAT、GSH-Px、ATP活性或水平均显著降低,凋亡率、ROS含量和MDA、IL-1β、TNF-α水平均显著增加(P<0.05或P<0.01),MMP明显降低。与模型组比较,溶剂对照组上述指标均无明显变化(P>0.05),紫铆花素高、中、低浓度组细胞存活率和SOD(除中、低浓度组外)、CAT、GSH-Px(除中、低浓度组外)、ATP(除低浓度组外)活性或水平均显著升高,凋亡率、ROS含量和MDA、IL-1β、TNF-α水平均显著降低(P<0.05或P<0.01),MMP明显增加。结论:紫铆花素可通过增强抗氧化酶活性,稳定线粒体功能,抑制氧化应激和炎症反应,增加能量生成,进而抑制神经细胞凋亡,最终发挥神经保护的作用。
ABSTRACT: OBJECTIVE:To study the protective effects of butein on oxidative stress injury of PC12 cell and its effects on mitochondrial function. METHODS:Rats PC12 cells were divided into normal control group,model group,solvent control group(1 ‰ dimethyl sulfoxide),butein high,medium and low concentration groups(2,1,0.5 μmol/L). The latter 4 groups were given relevant reagent/medicine for intervention;24 h later,other groups were given 100 mU/mL glucose oxidase to induce oxidant stress model except for normal control group. After 4 h culture,cell survival rate,apoptosis rate,the levels or activities of ROS,MDA,SOD,CAT,GSH-Px,ATP,IL-1β and TNF-α as well as the change of MMP were detected. RESULTS:Compared with normal control group,cell survival rate,the levels or activities of SOD,CAT,GSH-Px and ATP were all decreased significantly,and apoptotic rate,the content of ROS,the levels of MDA,IL-1β and TNF-α were all increased significantly(P<0.05 or P<0.01),while the MMP was decreased significantly. Compared with model group,above indexes of solvent control group had no significant change (P>0.05),cell survival rates,the levels or activities of SOD (except for medium and low concentration groups),CAT,GSH-Px(except for medium and low concentration groups),ATP(except for low concentration group)were increased significantly in butein high,medium and low concentration groups,while apoptotic rates,the content of ROS,the levels of MDA,IL-1 β and TNF-α were decreased significantly(P<0.05 or P<0.01),while the MMP were increased significantly. CONCLUSIONS:Butein can increase the antioxidant enzyme activity, stabilize mitochondrial function, inhibit oxidative stress and inflammationthus, increase energy generation inhibiting neuronal cell apoptosis ultimately exerting a neuroprotective effect.
期刊: 2020年第31卷第24期
作者: 刘珂娣,段佳林,苏晶,陶星茹,赵石,白杨,卫培峰,奚苗苗
AUTHORS: LIU Kedi,DUAN Jialin,SU Jing,TAO Xingru ,ZHAO Shi,BAI Yang,WEI Peifeng ,XI Miaomiao
关键字: 紫铆花素;PC12细胞;氧化应激;线粒体功能;炎症;细胞凋亡;神经保护
KEYWORDS: Butein; PC12 cell; Oxidative stress; Mitochondrial function;Inflammation;Apoptosis;Neuroprotection
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