UPLC-Q-TOF-MS/MS分析苗药云实皮的化学成分
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篇名: UPLC-Q-TOF-MS/MS分析苗药云实皮的化学成分
TITLE: Analysis of Chemical Compositions in Miao Medicine Caesalpinia decapetala by UPLC-Q-TOF-MS/MS
摘要: 目的:建立全面、快速分析苗药云实皮化学成分的方法,为该药质量控制和药效物质基础研究提供参考。方法:采用超高效液相色谱-四极杆-飞行时间串联质谱联用技术(UPLC-Q-TOF-MS/MS)。色谱柱为AgilentSB-C18,流动相为0.1%甲酸水溶液-0.1%甲酸乙腈溶液(梯度洗脱),流速为0.25mL/min,柱温为30℃,进样量为2µL;电喷雾离子源,正、负离子模式全程扫描,扫描范围为m/z50~1500,毛细管电压为4.5kV,雾化气(氮气)压力为1.2Bar,去溶剂气为氮气,去溶剂气流速为8L/min,去溶剂气温度为200℃。使用DataAnalysis4.2软件分析各成分峰的碎片离子信息,结合相关文献和对照品质谱图指认云实皮的化学成分。结果:在正离子模式下,共确定并指认9个化合物,峰1、2、3、4、5、6、7、8、9分别为儿茶素、原苏木素B、表儿茶素、没食子酸乙酯、槲皮素、木犀草素、3-去氧苏木查耳酮、异甘草素、亚油酸;在负离子模式下,共确定21个色谱峰,并指认了其中13个,峰3、4、5、6、7、8、9、10、11、12、13、15、21分别为儿茶素、短叶苏木酚酸、原苏木素B、表儿茶素、没食子酸乙酯、表儿茶素没食子酸酯、槲皮素、白藜芦醇、苏木查耳酮、木犀草素、3-去氧苏木查耳酮、异甘草素、亚油酸。结论:成功建立了可分析云实皮化学成分的UPLC-Q-TOF-MS/MS法。
ABSTRACT: OBJECTIVE:To estab lish a method that can comprehensively and rapidly analyze the chemical compositions of Miao medicine Caesalpinia decapetala,and to providing reference for quality control and pharmacodynamic material basis study of C. decapetala . METHODS :UPLC-Q-TOF-MS/MS was adopted . The determination was performed on Agilent SB-C 18 column with mobile phase consisted of 0.1% formic acid solution- 0.1% formic acid acetonitrile (gradient elution )at the flow rate of 0.25 mL/min. The column temperature was 30 ℃,and sample size was 2 µL. ESI source was applied in negative and positive scanning ion mode and data collection range of m/z 50-1 500. The capillary voltage was 4.5 kV,the atomizing gas (nitrogen)pressure was 1.2 Bar, the solvent removal gas was nitrogen ,the flow rate of solvent removal gas was 8 L/min and the solvent removal gas temperature was 200 ℃. Data Analysis 4.2 software was adopted to analyze fragment ion information of each peak ,and identify chemica l compositions on the basis of relevant literature and mass spectograms of reference substance. RESULTS :Under positive ion mode , 9 chemical compounds were identified ;peak 1,2,3,4,5,6,7,8,9 were catechin ,protohematoxylin B ,epicatechin,ethyl gallate,quercetin,luteolin,3-deoxy-hematoxylin chalcone , isoliquiritigenin and linoleic acid. Under negative ion mode , U1812403), totally 21 peaks were confirmed and 13 compounds were identified;peak 3,4,5,6,7,8,9,10,11,12,13,15, 21 were catechins , brevifolin carboxylic acid , proto- hematoxylin B ,epicatechin,ethyl gallate ,epicatechin gallate , quercetin,resveratrol,hematoxylin,luteolin,3-deoxy-hema- toxylin, isoliquiritigenin, linoleic acid. CONCLUSIONS UPLC-Q-TOF-MS/MS method is established successfully for analysis of chemical compositions in C. decapetala .
期刊: 2020年第31卷第20期
作者: 罗媛,王昌权,巩仔鹏,李月婷,王爱民,陈思颖,黄勇,郑林
AUTHORS: LUO Yuan, WANG Changquan,GONG Zipeng,LI Yueting,WANG Aimin,CHEN Siying,HUANG Yong,ZHENG Lin
关键字: 苗药;云实皮;超高效液相色谱-四极杆-飞行时间串联质谱联用技术;化学成分;分析鉴定
KEYWORDS: Miao medicine ; Caesalpinia decapetala; UPLC-Q-TOF-MS/MS; Chemical composition ; Analysis and
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