猪牙皂的萃取方法研究及萃取成分的GC-MS分析
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篇名: 猪牙皂的萃取方法研究及萃取成分的GC-MS分析
TITLE: Study on the Extraction Technology of Fructus Gleditisae Abnormalis and GC-MS Analysis of Extracted Com- ponents
摘要: 目的:建立猪牙皂的萃取方法并优化,同时分析鉴定萃取物的化学成分。方法:采用CO2超临界流体萃取(SFE)法对猪牙皂进行萃取;在单因素试验的基础上,以萃取率为评价指标,萃取温度、萃取压强、萃取时间为考察因素,采用正交试验优化SFE工艺并进行验证。采用气质联用技术对萃取物的化学成分进行鉴定,并采用面积归一化法计算各成分的相对百分含量。结果:猪牙皂的最优SFE萃取工艺为萃取温度60℃、萃取压强300MPa、萃取时间15min;3次验证试验的平均萃取率为1.73%(RSD=1.78%,n=3)。共鉴定出猪牙皂萃取物中的48个成分,占萃取物总量的98.31%;其中,有机酸类成分最多,占总成分的36.99%;其次是生物碱类成分,占总成分的12.59%;主要成分包括棕榈酸(16.62%)、油酸(14.12%)、N-氨基四氢吡咯(9.79%)、2,6-二甲基辛烷-1,7-二烯-3-醇(5.95%)、四氢吡喃(3.83%)、香兰素(3.39%)等。结论:成功建立猪牙皂萃取的SFE法,萃取物主要为有机酸类成分。
ABSTRACT: OBJECTIVE:To establish and optimize a extraction method of Fructus Gleditsiae Abnormallis ,and to analyze and identify chemical components of the extract simultaneously. METHODS :Fructus Gleditsiae Abnormallis was extracted with CO 2 supercritical fluid extraction (SFE)method. Based on single factor tests ,using extraction yield as index ,extraction temperature , extraction pressure and extraction time as investigation factors ,SFE technology was optimized with orthogonal test ,and validation test was performed. Chemical components in the extract were identified by GC-MS. Relative percentage of each component was calculated with area normalization method. RESULTS :The optimal SFE extraction technology of Fructus Gleditsiae Abnormallis was extraction temperature of 60 ℃,extraction pressure of 300 MPa and pression time of 15 min. Average extraction of 3 times of validation tests was 1.73%(RSD=1.78%,n=3). The 48 components in the extracts of Fructus Gleditsiae Abnormallis were identified,which accounted for 98.31% of the total amount of the extracts. The extracts of Fructus Gleditisae Abnormalis mainly included organic acids ,accounting for 36.99%,followed by alkaloids ,accounting for 12.59% in total. Main components were palmitic acid (16.62%),oleic acid (14.12%),N-aminotetrahydropyrrole(9.79%),2,6-dimethyloctane-1,7-dien-3-ol(5.95%), tetrahydropyran(3.83%),vanillin(3.39%),etc. CONCLUSIONS :SFE method of Fructus Gleditisae Abnormalis is established successfully,and the extract is mainly organic acids.
期刊: 2020年第31卷第20期
作者: 邓铋莉,董娜娜,王旭兰,罗成月,陈晓兰
AUTHORS: DENG Bili,DONG Nana,WANG Xulan,LUO Chengyue ,CHEN Xiaolan
关键字: 猪牙皂;CO2超临界流体萃取法;单因素试验;正交试验;气质联用技术
KEYWORDS: Fructus Gleditisae Abnormalis ;CO2 supercritical fluid extraction ;Single factor test ;Orthogonal test ;GC-MS
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