蒙药地梢瓜的质量标准研究
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篇名: 蒙药地梢瓜的质量标准研究
TITLE: Study on the Quality Standards of Mongolian Medicine Cynanchum thesioides
摘要: 目的:建立蒙药地梢瓜的质量标准。方法:采用薄层色谱法(TLC)鉴别地梢瓜药材;按2015年版《中国药典》(四部)方法检测药材中的水分、总灰分、醇溶性浸出物含量;采用高效液相色谱法(HPLC)测定药材中地梢瓜苷的含量。结果:TLC图斑点清晰,供试品(地梢瓜药材)与对照品(地梢瓜苷)在相应位置上均显示相同的黄绿色荧光斑点。22批地梢瓜药材的水分含量为6.18%~12.97%,总灰分含量为4.64%~7.95%,醇溶性浸出物含量为12.46%~32.70%。地梢瓜苷检测进样量的线性范围为0.048~3.050μg(R2=0.9999);精密度、稳定性、重复性、耐用性试验的RSD均小于1%;加样回收率为104.03%~106.36%(RSD=0.96%,n=6);22批地梢瓜药材中地梢瓜苷的含量为0.0062%~0.1305%。结论:初步拟定地梢瓜中水分不得超过11.50%,总灰分不得超过7.50%,醇溶性浸出物不得少于17.00%,地梢瓜苷含量不得少于0.05%。所建质量标准可用于蒙药地梢瓜药材的质量控制。
ABSTRACT: OBJECTIVE:To establish the quality standards of Mongolian medicine Cynanchum thesioides. METHODS :TLC was used for the qualitative identification of C. thesioides . According to 2015 edition of Chinese Pharmacopeia (part Ⅳ),the moisture,total ash and ethanol-soluble extract were determined. HPLC method was used to determine the content of thesioideoside in C. thesioides . RESULTS :TLC spots were clear ,there were same yellow green fluorescent spots on the corresponding position of the sample (C. thesioides )and control (thesioideoside). In 22 batches of samples ,contents of moisture were 6.18%-12.97%,total ash were 4.64%-7.95%,ethanol-soluble extract were 12.46%-32.70%. The linear range of thesioideoside were 0.048-3.050 μg(R2= 0.999 9). RSDs of precision , stability, repeatability and durability tests were all less than 1% . The recoveries were 104.03%-106.36%(RSD=0.96%,n=6). The contents of thesioideoside in 22 batches of C. thesioides were 0.006 2%-0.130 5%. CONCLUSIONS:It is suggested that the moisture and total ash should not exceed 11.50% and 7.50%,respectively;the contents of ethanol-soluble extract and the sioideoside are no less than 17.00% and 0.05%,respectively. The established quality standards can be used for quality control of Mongolian medicine C. thesioides .
期刊: 2020年第31卷第18期
作者: 吴依静,喜杰,钟国跃,苏日娜,李辉虎,罗维早
AUTHORS: WU Yijing,XI Jie,ZHONG Guoyue ,SU Rina,LI Huihu,LUO Weizao
关键字: 蒙药;地梢瓜;地梢瓜苷;薄层色谱法;高效液相色谱法;含量测定;质量标准
KEYWORDS: Mongolian medicine ;Cynanchum thesioides;Thesioideoside;TLC;HPLC;Content determination ;Quality
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