金莲花不同溶剂提取物抗心肌细胞缺氧/复氧损伤的谱-效关系研究
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篇名: 金莲花不同溶剂提取物抗心肌细胞缺氧/复氧损伤的谱-效关系研究
TITLE: Study on the Spectrum-effect Relationship of Different Solvent Extracts from Trollius chinensis against Hypoxia/ reoxygenation Injury of Cardiomyocyte
摘要: 目的:研究金莲花11种不同溶剂提取物抗心肌细胞缺氧/复氧损伤的谱-效关系。方法:利用高效液相色谱-串联质谱法建立金莲花11种不同溶剂提取物的指纹图谱,通过与对照品、文献信息比对,指认共有峰对应的化合物。采用MTT法检测金莲花11种不同溶剂提取物对缺氧/复氧损伤大鼠心肌细胞H9c2存活率的影响,采用酶联免疫吸附测定法检测细胞中丙二醛(MDA)含量、活性氧(ROS)水平和上清液中乳酸脱氢酶(LDH)含量。通过灰色关联度分析法(GRA)和偏最小二乘法(PLS)联合分析共有峰对应成分与抗心肌细胞缺氧/复氧损伤(药效)的谱-效关系。结果:金莲花11种不同溶剂提取物的指纹图谱中有22个共有峰,指认出22个化合物。与缺氧/复氧损伤组比较,缺氧/复氧+S1~S6、S9、S10组细胞的存活率均显著升高,MDA含量、ROS水平、LDH含量均显著降低(P<0.05);缺氧/复氧+S8组细胞的ROS水平、LDH含量均显著降低(P<0.05)。22个化合物与药效的GRA关联度均大于0.8;除峰1、2、7、13、14、21外,其余峰对应化合物与药效的PLS分析回归系数均大于0(即成正相关);贡献排名前9位的共有峰均为峰6>11>4>5>8>9>12>10>15。结论:荭草苷(峰6)、牡荆苷(峰11)、荭草素-2″-O-β-L-半乳糖苷(峰4)、荭草素-2″-O-β-D-吡喃木糖苷(峰5)、槲皮素-3-O-吡喃葡萄糖苷(峰8)、牡荆素-2″-O-β-L-半乳糖苷(峰9)、金丝桃苷(峰12)、牡荆素-2″-O-β-D-吡喃木糖苷(峰10)、2″-O(-2″′-甲基丁酰基)-荭草苷(峰15)可能是金莲花抗心肌细胞缺氧/复氧损伤的主要成分。
ABSTRACT: OBJECTIVE:To study spectrum-effect relationship of 11 different solvent extracts from Trollius chinensis against hypoxia/reoxygenation injury of cardiomyocyte . METHODS :HPLC-MS/MS method was used to establish the fingerprints of 11 different solvent extracts from T. chinensis ,the compounds corresponding to the common peaks were identified by comparing with the substance control and literature information. MTT assay was used to detect the effects of 11 different solvent extracts from T. chinensis on the survival rate of rat myocardial H 9c2 cells injured by hypoxia/reoxygenation. The MDA content ,ROS level in cells and LDH content in the supernatant were detected by ELISA. GRA and PLS method were used to analyze the spectrum-effect relationship between the compounds corresponding to common peak and anti-hypoxia/reoxygenation injury of cardiomyocyte (drug effect). RESULTS :There were 22 common peaks in 11 different solvent extracts from T. chinensis ,and 22 compounds were identified. Compared with hypoxia/reoxygenation injury group ,survival rate of hypoxia/reoxygenation injury+S 1-S6,S9 and S 10 groups were increased significantly ,while MDA content ,ROS level and LDH content were decreased significantly (P<0.05); ROS level and LDH content of hypoxia/reoxygenation injury+S 8 group w ere decreased significantly (P<0.05). The r of GRA analysis of 22 compounds with drug effects were all higher than 0.8. Except for peaks 1,2,7,13,14 and 21,r of PLS analysis of rest peaks with drug effects were higher than 0 发。电话:0431-86058683。E-mial:nml2000@163.com (being positive correlation ). Top 9 common peaks in the list of contribution rate were peak 6>11>4>5>8>9>12>10>15. CONCLUSIONS :Orientin(peak 6),vitexin(peak 11), orientin-2″-O-β-L-galacto- pyranosl (peak 4),orientin-2″-O-β-D-Pyrine xylosides (peak 5),quercetin-3-O-glucopyranoside(peak 8),vitexin-2″-O-β-L-galactoside(peak 9),hyperoside(peak 12),vitexin-2″-O-β-D-pyrine xylosides (peak 10),2″-O-(2″′- methylbutyry-loxy)-orientin(peak 15)may be the main components of anti-hypoxia/reoxygenation injury of cardiomyocytes.
期刊: 2020年第31卷第18期
作者: 南敏伦,孙琦,杨振,司学玲,马春霞,白雪,赫玉芳
AUTHORS: NAN Minlun ,SUN Qi,YANG Zhen,SI Xueling ,MA Chunxia ,BAI Xue,HE Yufang
关键字: 金莲花;心肌细胞H9c2;缺氧/复氧损伤;谱-效关系;灰色关联度分析法;偏最小二乘法
KEYWORDS: Trollius chinensis ;Myocardial cells H 9c2;Hypoxia/reoxygenation injury ;Spectrum-effect relationship ;GRA;PLS
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