Box-Behnken设计-响应面法优化滇龙胆药材产地初加工工艺
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篇名: Box-Behnken设计-响应面法优化滇龙胆药材产地初加工工艺
TITLE: Optimization of Primary Processing Technology of Gentiana rigescens by Box-Behnken Design-response Surface Method
摘要: 目的:优化滇龙胆药材的产地初加工工艺。方法:采用高效液相色谱法测定滇龙胆中马钱苷酸、獐牙菜苦苷和龙胆苦苷的含量,再以上述3种成分含量的总评归一值(OD值)为评价指标,对热烫温度、热烫时间和干燥温度进行单因素试验;在此基础上,采用Box-Behnken设计-响应面法优化滇龙胆药材产地初加工工艺并进行验证,再与阴干样品进行比较。结果:滇龙胆药材产地初加工最优工艺为热烫时间5min,热烫温度40℃,烘干温度60℃。验证试验结果显示,滇龙胆药材中3种成分的平均OD值为0.5652,与预测值(0.5706)的偏差为0.94%。与阴干样品比较,滇龙胆最优工艺样品中3种成分含量的OD值明显升高,表明优化工艺加工的滇龙胆样品质量更好。结论:优化的工艺稳定、可行,可用于滇龙胆药材的产地初加工。
ABSTRACT: OBJECTIVE:To optimize the primary processing technology of Gentiana rigesce ns. METHODS :HPLC method was adopted for content determination of loganic acid ,swertiamarin and gentiopicroside in G. rigescens ,and overall desirability value (OD value ) of the contents of above 3 components was taken as index to carry out single factor test on blanching temperature,blanching time and drying temperature. Based on that ,Box-Behnken design-response surface methodology was used to optimize primary processing technology of G. rigescens . Validation test was also performed. The samples prepared by optimized technology were compared with those dried in the shade. RESULTS :The optimal primary processing technology of G. rigescens included blanching time of 5 min,blanching temperature of 40 ℃ and drying temperature of 60 ℃. Validation test showed that the average OD value of the 3 components was 0.565 2,with a deviation of 0.94% from the predicted value (0.570 6). Compared with samples dried in the shade ,OD value of 3 components in samples prepared by optimized technology were increased significantly , indicating the quality of the samples prepared by the optimized technology was better. CONCLUSIONS :The optimal technology is stable and feasible ,and can be used for the primary processing of G. rigescens .
期刊: 2020年第31卷第15期
作者: 吴昕怡,刘秀嶶,屈云慧,石萍萍,杨雁,李智敏,田浩,谷丽萍
AUTHORS: WU Xinyi,LIU Xiuwei ,QU Yunhui ,SHI Pingping ,YANG Yan,LI Zhimin ,TIAN Hao,GU Liping
关键字: 滇龙胆;产地初加工;Box-Behnken设计;响应面法;总评归一值
KEYWORDS: Gentiana rigescens ;Primary processing technology ;Box-Behnken design ;Response surface method ;Overall
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