昆明山海棠提取物对新西兰兔的胚胎-胎仔发育毒性研究
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篇名: 昆明山海棠提取物对新西兰兔的胚胎-胎仔发育毒性研究
TITLE: Study on Toxic Effect of the Extract from Tripterygium hypoglaucum on Embryo-fetal Development of New Zealand Rabbits
摘要: 目的:研究昆明山海棠提取物对妊娠动物、胚胎及胎仔发育的影响。方法:取交配成功的新西兰雌兔,按体质量随机区组法分为溶剂对照组、阳性对照组(环磷酰胺,20mg/kg)和昆明山海棠提取物高、中、低剂量组(15.0、7.50、3.75g/kg,以生药量计),每组均至少18只。溶剂对照组和昆明山海棠提取物各剂量组雌兔在妊娠第6~18天灌胃水或相应昆明山海棠提取物药液,5mL/kg,每天1次;阳性对照组雌兔在妊娠第10~13天于颈部皮下注射环磷酰胺,1mL/kg,每天1次。依据《药物生殖毒性研究技术指导原则》相关要求,在实验期间观察并记录雌兔的一般情况、体质量和摄食量,并于妊娠第28天时对其实施安乐死,解剖后观察并记录其主要脏器、带胎子宫、胎盘子宫、胎盘、卵巢以及着床腺、吸收胎、死胎、活胎、黄体等相关指标,并对胎仔进行体质量、外观形态、内脏和骨骼发育等相关指标的检查。结果:与溶剂对照组比较,昆明山海棠提取物各剂量组孕兔的体质量、体质量增长、摄食量、主要脏器,孕兔妊娠、生殖功能以及胚胎形成和胎仔的生长发育、外观形态、内脏和骨骼发育等相关指标均未见明显异常改变(P>0.05);阳性对照组上述指标均有显著变化(P<0.05),表现出明显的孕兔母体毒性以及胚胎和胎仔发育毒性。结论:昆明山海棠提取物在剂量为3.75~15.00g/kg(以生药量计)时对新西兰兔无明显母体毒性、胚胎或胎仔发育毒性。
ABSTRACT: OBJECTIVE:To study the toxic effect of the extract from Tripterygium hypoglaucum on pregnant animal and embryo-fetal development . METHODS :Successfully mated New Zealand female rabbits were randomly divided into solvent control group,positive control group (cyclophosphamide,20 mg/kg)and T. hypoglaucum extract high-dose ,medium-dose and low- dose groups(15.0,7.50,3.75 g/kg,by the amount of crude drug )according to their body weight ,with 18 rabbits in each group at least. The female rabbits in solvent control group and T. hypoglaucum extract groups were given water or the corresponding T. hypoglaucum extract solution from 6th to 18th day of pregnancy ,5 mL/kg,once a day. Positive control group was given cyclophosphamide subcutaneously into the neck from 10th to 13th day of pregnancy ,1 mL/kg,once a day. According to the related requirements of Technical Guidelines for the Study of Drug Reproductive Toxicity ,the general situation ,body weight ,body weight increase and food intake of female rabbits were observed and recorded during the experiment ,and euthanasia was carried out on 28th day of pregnancy ;the relative indexes of main organs ,fetal uterus ,placenta uterus ,placenta,ovary,implantation gland , absorption fetus ,stillbirth,live fetus and corpus luteum were observed and recorded after anatomy ;the relative indexes of body weight,appearance,visceral and skeletal alterations of the fetus were detected . RESULTS :Compared with solvent control group , the body weight ,body weight increase ,food intake ,main organs ,pregnancy of pregnant rabbits ,as well as reproductive function,embryo formation ,fetal growth and development ,appearance,visceral and skeletal development indexes in T. hypoglaucum extract groups had no significant abnormal changes (P>0.05);above indexes in the positive control group had significant changes (P<0.05),and significant maternal toxicity and embryotoxicity were found. CONCLUSIONS :T. hypoglaucum extract 15.00-3.75 g/kg(by the amount of crude drug )have no significant maternal toxicity ,embryotoxicity or fetal development toxicity to New Zealand rabbits.
期刊: 2020年第31卷第14期
作者: 施汀兰,张莉,黄鹤,梅小利,涂如霞,吴思澜,兰波,李恒华
AUTHORS: SHI Tinglan ,ZHANG Li,HUANG He,MEI Xiaoli,TU Ruxia,WU Silan,LAN Bo,LI Henghua
关键字: 昆明山海棠;提取物;新西兰兔;胚胎;胎仔;生殖毒性
KEYWORDS: Tripterygium hypoglaucum;Extract;New Zealand rabbits ;Embryo;Fetal;Reproductive toxicity
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