柴胡疏肝散辅助治疗重度抑郁症的网络药理学研究
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篇名: 柴胡疏肝散辅助治疗重度抑郁症的网络药理学研究
TITLE: Study on Chaihu Shugan Powder in the Adjunctive Treatment of Major Depressive Disorder Based on Network Pharmacology
摘要: 目的:研究柴胡疏肝散辅助治疗重度抑郁症(MDD)的潜在分子机制。方法:采用网络药理学方法。利用基因表达综合数据库(GEO)下载MDD患者基因芯片数据集GSE76826,再利用R语言软件中的limma包筛选出MDD患者样本中的差异基因。利用中药系统药理学数据库和分析平台(TCMSP)筛选柴胡疏肝散的活性成分及其对应的靶标。通过在线软件venny2.1.0对MDD的差异基因和柴胡疏肝散的靶标取交集,获取柴胡疏肝散治疗MDD的共同靶标基因,然后将共同靶标基因导入Cytoscape3.6.1软件构建“柴胡疏肝散活性成分-MDD靶标基因”关系网络。利用BisoGenet插件构建蛋白互作(PPI)网络,并对其进行拓扑结构分析,筛选核心靶标基因,进行京都基因与基因组百科全书(KEGG)通路分析。结果:柴胡疏肝散治疗MDD的活性成分有139种,包括槲皮素、山柰酚、木犀草素、豆甾醇等;其作用的核心靶点基因共有8个,包括NR3C2、DPP4、ALOX5、GSTM1、CCND1、IGFBP3等;其主要通过调节细胞受体、嘌呤代谢、类固醇生物合成、氨酰生物合成等信号通路发挥治疗MDD的作用。结论:柴胡疏肝散可通过多个靶标、多个途径发挥辅助治疗MDD的作用,可为后续研究其作用机制提供理论基础。
ABSTRACT: OBJECTIVE:To explore the mo lecular mechanism of Chaihu shugan powder in the adjunctive treatment of major depressive disorder (MDD). METHODS :Network pharmacology was adopted. The microarray dataset GSE 76826 was downloaded from GEO common database ,and the limma package was used to screen the differently expressed genes in the samples of MDD patients. The active ingredients and corresponding targets of Chaihu shugan powder were screened out based on TCMSP platform. Through the online software venny 2.1.0,the differently expressed genes of MDD and the targets of Chaihu shugan powder were screened,and the common target genes of Chaihu shugan powder for MDD were obtained. The target genes were introduced into Cytoscape 3.6.1 software to construct the network diagram of “Chaihu shugan powder active ingredients-MDD target gene ”. Then , BisoGenet plug-in was used to construct the PPI network ,analyze the topological structure and screen the core target gene. KEGG pathway analysis was conducted. RESULTS :There were 139 active components of Chaihu shugan powder for the treatment of MDD,including quercetin ,kaempferol,luteolin,stigmasterol,etc. There were 8 core target genes ,including NR3C2,DPP4, ALOX5,GSTM1,CCND1,IGFBP3 and so on. They play an important role in the treatment of MDD via signaling pathway such as regulating cell receptor ,purine metabolism ,steroid biosynthesis ,aminoacyl biosynthesis ,etc. CONCLUSIONS :Chaihu shugan powder can play a role in the adjunctive treatment of MDD through multiple targets and pathways ,which can provide a theoretical basis for the follow-up study of its mechanism.
期刊: 2020年第31卷第13期
作者: 卢玲,庄胜,陈炜,玉倩,汪顺贵,李华霞,刁丽梅
AUTHORS: LU Ling,ZHUANG Sheng,CHEN Wei,YU Qian,WANG Shungui ,LI Huaxia ,DIAO Limei
关键字: 网络药理学;柴胡疏肝散;重度抑郁症;靶标;机制
KEYWORDS: Network pharmacology ; Chaihu shugan
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