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苗药铁筷子不同炮制品中4种成分含量及毒性的比较研究
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| 篇名： | 苗药铁筷子不同炮制品中4种成分含量及毒性的比较研究 |
| TITLE： | Comparative Study on the Contents of Four Compounds from Different Processed Products of Miao Medicine Chimonanthus nitens and Its Toxicity |
| 摘要： | 目的：比较苗药铁筷子阴干、烘干、蒸制、炒炭制品中4种成分的含量及其毒性。方法：采用高效液相色谱法（HPLC）同时测定铁筷子炮制品中东莨菪苷、秦皮苷、东莨菪内酯、异嗪皮啶的含量。色谱柱为Welch-C18，流动相为乙腈-水（梯度洗脱），流速为1mL/min，检测波长为214nm，柱温为30℃，进样量为10μL。采用改良寇氏法测定铁筷子不同加工炮制品对小鼠的半数致死量（LD50）及其95％置信区间（CI），并评价其急性毒性。结果：东莨菪苷、秦皮苷、东莨菪内酯、异嗪皮啶检测质量的线性范围分别为0.019～1.856、0.016～1.616、0.009～0.920、0.006～0.624μg（R2均不低于0.9995），精密度、稳定性、重复性试验的RSD均小于2.0％（n＝6）；平均加样回收率分别为104.49％、102.22％、101.45％、99.26％（RSD均小于2％，n＝6）；阴干、烘干、蒸制、炒炭品中，东莨菪苷含量分别为1.1190％、0.9043％、1.0684％、0.0364％，秦皮苷含量分别为0.8678％、0.4539％、0.4237％、0.0205％，东莨菪内酯含量分别为0.0771％、0.1555％、0.0844％、0.2908％，异嗪皮啶含量分别为0.1102％、0.2021％、0.2081％、0.2494％；4种炮制品的LD50分别为4118.13、3733.36、1643.61、＞10000mg/kg，95％CI分别为（3748.87，4523.76）、（3422.16，4072.86）、（1520.90，1776.23）、（＞10000）mg/kg。结论：HPLC法重复性、精密度较好，可用于同时测定铁筷子不同炮制品中4种成分的含量；不同炮制品中4种成分均有所差异，2个糖苷类毒性成分的含量明显下降；4种炮制品均为低毒或无毒。 |
| ABSTRACT： | OBJECTIVE： To compare the contents of 4 components and toxicity from Miao medicine Chimonanthus nitens samples after drying in the shade ，oven drying ，steam，charring. METHODS ：The contents of scopolin，fraxin，scopoletin and isofraxin were simultaneously determined by HPLC. The separation was performed on Welch-C18 column with mobile phase consisted of acetonitrile-water （gradient elution ）at the flow rate of 1 mL/min. The detection wavelength was set at 214 nm，and column temperature was 30 ℃，and the sample size was 10 μL. Modified Karber method was used to determine the LD 50 and its 95％CI of different processed C. nitens to mice and evaluate its acute toxicity. RESULTS ： The linear range of scopolin ， fraxin， scopoletin and isofraxin were 0.019-1.856，0.016-1.616，0.009-0.920，0.006-0.624 μg（R2 were all not lower than 0.999 5）. RSDs of precision ， stability and reprodu-cibility tests were all lower than 2.0％（n＝6）. The average recoveries were 104.49％，102.22％， 101.45％，99.26％（all RSDs were lower than 2％，n＝6）. The contents of scopolin were 1.119 0％，0.904 3％， 1.068 4％and 0.036 4％；those o f fraxin were 0.867 8％，0.453 9％，0.423 7％and 0.020 5％；those of scopoletin ； those of isofraxidin were 0.110 2％，0.202 1％，0.208 1％ and 0.249 4％in samples after drying in the shade ，oven CX-2018-001） drying， steam， charring. The LD 50 of 4 processedproducts were 4 118.13，3 733.36，1 643.61，＞10 000 qq.com mg/kg samples ；95％CI were （3 748.87，4 523.76）， （3 422.16，4 072.86），（1 520.90，1 776.23），（＞10 000） mg/kg. CONCLUSIONS ：HPLC method is reproducible and precise. It can be used to determine the contents of 4 components in different processed products of C. niten s. The contents of 4 components in different processed products ， and the contents of glycosides toxicity components are decreased significantly. All the 4 processed products were low or non-toxic. |
| 期刊： | 2020年第31卷第12期 |
| 作者： | 王雪，王丽，廖秀，王海波，杨娟，李齐激，杨小生 |
| AUTHORS： | WANG Xue，WANG Li，LIAO Xiu，WANG Haibo，YANG Juan，LI Qiji，YANG Xiaosheng |
| 关键字： | 铁筷子；炮制；含量；急性毒性；糖苷类成分；小鼠 |
| KEYWORDS： | Chimonanthus nitens ；Processing；Content；Acute toxicity ；Glycosides；Mice |
| 阅读数： | 295 次 |
| 本月下载数： | 6 次 |
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