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正交试验结合信息熵赋权法优化千里光药材的提取工艺
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| 篇名： | 正交试验结合信息熵赋权法优化千里光药材的提取工艺 |
| TITLE： | Optimization of the Extraction Technology of Senecio scandens by Orthogonal Design Combined with Informa- tion Entropy Weighting Method |
| 摘要： | 目的：优化千里光药材的提取工艺，为其进一步开发利用提供方法参考。方法：采用80℃水浴回流提取的方法提取千里光药材。采用高效液相色谱法测定绿原酸和金丝桃苷的含量，色谱柱为DiamonsilC18，流动相为0.2％冰醋酸水溶液-甲醇（82∶18，V/V），柱温为35℃，流速为1.0mL/min，检测波长分别为327nm，进样量为5μL；采用紫外-可见分光光度法测定总黄酮（以芦丁计）和总生物碱（以苦参碱计）的含量，检测波长分别为509、208nm。在单因素试验的基础上，以乙醇体积分数（A，％）、溶剂倍量（B，mL/g）、提取时间（C，h）、提取次数（D，次）为考察因素，以绿原酸、金丝桃苷、总黄酮和总生物碱的含量为评价指标，通过信息熵赋权法计算上述各指标的权重系数并以此计算其综合评分，按L（934）正交试验设计优化千里光提取工艺并进行验证。结果：千里光药材的最佳提取工艺为加入8倍量50％乙醇，回流提取3次，每次1.5h；3次验证试验结果显示，所得提取液中绿原酸、金丝桃苷、总黄酮和总生物碱含量的RSD均小于1.5％（n＝3）。结论：优化的提取工艺重复性好、稳定可行，可用于千里光药材的提取。 |
| ABSTRACT： | OBJECTIVE：To optimize the extraction technology of Senecio scandens ，and to provide reference for the further development and utilization of the medicinal material. METHODS ：The method of reflux extraction with 80 ℃ water bath was used to extract S. scandens . HPLC method was adopted to determine the contents of chlorogenic acid and hyperoside. The determination was performed on Diamonsil C 18 with mobile phase consisted of 0.2％ glacial acetic acid water solution-methanol （82∶18，V/V）at the flow rate of 1.0 mL/min；the column temperature was set at 35 ℃；the detection wavelength was 327 nm，and the sample size was 5 μL. UV-Vis spectrophotometry was used to determine the contents of total flavonoids（by rutin ）and total alkaloids （by matrine）and the detection wavelengths were set at 509 nm and 208 nm，respectively. Based on single factor tests ，using ethanol volume fraction （A，％），solvent folds （B，mL/g），extraction time （C，h），extraction times （D，times）as factors ，using the contents of chlorogenic acid ，hyperoside，total flavonoids and total alkaloids as indexes ，and t he weight coefficients of above indicator components were calculated based on information entropy weighting method so as to calculate their comprehensive scores ， then L 9（34）orthogonal design was used to further optimize the extraction technology. The validation tests were also performed. RESULTS：The optimal extraction technology of S. scandens included that 8-fold 50％ ethanol，extracting for 3 times，lasting for 1.5 h each time. Results of 3 times of validation tests showed that RSDs of the contents of chlorogenic acid ，hyperoside，total flavonoids and total alkaloids were all lower than 1.5％（n＝3）. CONCLUSIONS ：The optimized technology is reproducible ， stable and feasible ，and can be used for the extraction of S. scandens . |
| 期刊： | 2020年第31卷第12期 |
| 作者： | 李燕，潘炎帆，张涛，韩忠耀 |
| AUTHORS： | LI Yan，PAN Yanfan ，ZHANG Tao，HAN Zhongyao |
| 关键字： | 千里光；信息熵赋权法；绿原酸；金丝桃苷；总黄酮；总生物碱；含量；正交试验；提取工艺 |
| KEYWORDS： | Senecio scandens ；Information entropy weighting theory ；Chlorogenic acid ；Hyperoside；Total flavonoids ；Total |
| 阅读数： | 478 次 |
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