美洲大蠊提取物CⅡ-3对MFC荷瘤小鼠抑瘤机制的1H-NMR代谢组学研究
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篇名: 美洲大蠊提取物CⅡ-3对MFC荷瘤小鼠抑瘤机制的1H-NMR代谢组学研究
TITLE: Study on Antitumor Mechanism of Periplaneta americana Extract C Ⅱ-3 on MFC Tumor-bearing Mice Based on 1H-NMR Metabonomics
摘要: 目的:初步研究美洲大蠊提取物CⅡ-3对上皮肿瘤细胞MFC荷瘤小鼠的抑瘤机制。方法:将Balb/c小鼠随机分为模型组(生理盐水20mL/kg)和CⅡ-3组(200mg/kg),每组6只。在小鼠右侧腋下注射MFC细胞悬液0.2mL后,于次日灌胃相应药物,每日1次,连续10d。末次给药24h后,在测量瘤体大小的基础上,采用氢-1核磁共振技术(1H-NMR)并结合非监督型主成分分析(PCA)、监督型偏最小二乘法-判别分析(PLS-DA)和正交偏最小二乘法-判别分析(OPLS-DA),比较两组荷瘤小鼠肝组织的代谢谱并分析差异性代谢物,探索CⅡ-3抑瘤作用的潜在机制。结果:与模型组比较,CⅡ-3组荷瘤小鼠的瘤体明显减小;两组小鼠的1H-NMR图谱存有差异;结合非监督型PCA、监督型PLS-DA、OPLS-DA结果,共确定了肝组织潜在差异性代谢物6个,分别为糖原(升高)、丙酮酸(降低)、精氨酸(降低)、羟脯氨酸(升高)、肌苷(升高)、烟酰胺(升高),主要归属于精氨酸代谢、能量代谢和核酸代谢。结论:CⅡ-3对MFC荷瘤小鼠的抑瘤作用可能与调节精氨酸代谢、能量代谢和核酸代谢有关。
ABSTRACT: OBJECTIVE:To preliminarily study the antitumor mechanism of Periplaneta americana extract C Ⅱ-3 on MFC tumor-bearing mice. METHODS :Balb/c mice were randomly divided into model group (normal saline 20 mL/kg)and C Ⅱ-3 group (200 mg/kg),with 6 mice in each group. MFC cell suspension (0.2 mL)was injected under the right armpit of mice. On the next day,mice were given relevant medicine intragastrically ,once a day ,for consecutive 10 d. 24 h after the last administration ,Based on the measurement of tumor size , 1H-NMR technology combined with unsupervised PCA ,supervised PLS-DA and OPLS-DA were used to compare metabolic spectrum of liver tissue from tumor-bearing mice of 2 groups,to analyze differential metabolites and to explore the potential antitumor mechanis m of C Ⅱ -3. RESULTS :Compared with model group ,the tumor body was significantly reduced in tumor-bearing mice of C Ⅱ-3 group. There were differences in 1H-NMR spectra between the 2 No.81960712); groups. According to unsupervised PCA ,supervised PLS-DA and OPLS-DA ,totally six potential differential metabolites ,as glycogen (increased),pyruvate (decreased),arginine (de- creased),hydroxyproline (increased),inosine (increased) and niacinamide (increased),were identified in the liver tissue,which were mainly attributed to the metabolism of arginine ,energy and nucleic acid. CONCLUSIONS:The anti tumor effect of C Ⅱ-3 may be related to the regulation of arginine metabolism ,energy metabolism and nucleic acid metabolism.
期刊: 2020年第31卷第12期
作者: 厉颖,陶柱萍,常旭,欧红利,江伦,李灿委,唐艳隆,周玥,白丽,高鹏飞
AUTHORS: LI Ying,TAO Zhuping, CHANG Xu,OU Hongli,JIANG Lun,LI Canwei,TANG Yanlong,ZHOU Yue,BAI Li,GAO Pengfei
关键字: 美洲大蠊提取物CⅡ-3;上皮肿瘤细胞MFC;代谢组学;氢-1核磁共振技术;小鼠
KEYWORDS: Periplaneta americana extract C Ⅱ-3;MFC cell line ;Metabolomics;1H-NMR;Mice
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