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基于“增效-减毒”的附子盐制辅料的比较研究
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| 篇名： | 基于“增效-减毒”的附子盐制辅料的比较研究 |
| TITLE： | Comparative Study on the Salt-processed Excipients for Aconite Based on “Efficacy Enhancing and Toxicity Re- ducing” |
| 摘要： | 目的：比较生附子及其5种盐制炮制品中6种酯型生物碱成分的含量，为优化附子盐制工艺提供参考。方法：分别以胆巴、氯化镁、氯化钠、氯化钙、氯化钾为辅料炮制生附子，并采用高效液相色谱法测定生附子及上述5种盐制炮制品中6种酯型生物碱的含量；采用效/毒比、毒性成分指数评价生附子及其5种盐制炮制品的效果和毒性。结果：苯甲酰新乌头碱、苯甲酰次乌头碱、苯甲酰乌头碱、新乌头碱、乌头碱、次乌头碱检测质量浓度的线性范围分别为2.440～24.40、2.240～22.40、2.020～20.20、2.780～27.80、2.240～22.40、2.240～22.40μg/mL（r≥0.9990）；精密度、稳定性、重复性试验的RSD均小于2％；加样回收率分别为102.13％～104.87％（RSD＝0.98％，n＝6）、100.00％～105.00％（RSD＝2.02％，n＝6）、95.00％～99.29％（RSD＝1.77％，n＝6）、100.41％～104.58％（RSD＝1.78％，n＝6）、98.87％～99.90％（RSD＝0.41％，n＝6）、100.20％～104.00％（RSD＝1.55％，n＝6）。总生物碱含量由高到低依次为生附子＞氯化钠炮制品＞胆巴炮制品＞氯化钾炮制品＞氯化钙炮制品＞氯化镁炮制品。效/毒比由大到小依次为氯化钾炮制品＞氯化镁炮制品＞氯化钠炮制品＞氯化钙炮制品＞胆巴炮制品＞生附子；毒性成分指数由大到小依次为生附子＞氯化钠炮制品＞胆巴炮制品＞氯化钙炮制品＞氯化钾炮制品＞氯化镁炮制品。结论：以5种盐为辅料的炮制方法均具有不同程度的“增效-减毒”功效，其中以氯化镁、氯化钾、氯化钙炮制效果为佳，可用于附子的炮制。 |
| ABSTRACT： | OBJECTIVE：To compare the c ontents of 6 kinds of ester alkaloids in raw aconite and 5 kinds of salt-processed products，and to provide reference for the optimization of salt-processing technology. METHODS ：Processed with bittern ， magnesium chloride ，sodium chloride ，calcium chloride and potassium chloride as excipients ，and HPLC method were adopted to determine the contents of 6 kinds of ester alkaloids in raw aconite and 5 kinds of salt-processed products. The effect/toxicity ratio and toxicity component index were used to evaluate the effect and toxicity of raw aconite and 5 kinds of salt-processed products. RESULTS： The linear range of benzoylneoaconitine ， benzoylhypoconitine， benzoylaconitine， neoaconitine， aconitine and hypoconitine were 2.440-24.40，2.240-22.40，2.020-20.20，2.780-27.80，2.240-22.40，2.240-22.40 μg/mL（r≥0.999 0）. RSDs of precision，stability and repeatability tests were all less than 2％. The recovery rates were 102.13％-104.87％（RSD＝0.98％，n＝ 6），100.00％-105.00％（RSD＝2.02％，n＝6），95.00％-99.29％（RSD＝1.77％，n＝6），100.41％-104.58％（RSD＝1.78％，n＝ 6），98.87％-99.90％（RSD＝0.41％，n＝6），100.20％-104.00％（RSD＝1.55，n＝6），respectively. The contents of total alkaloids in order from the largest to the smallest was as follows ：raw aconite ＞processed products of sodium chloride ＞processed products of bittern ＞processed products of potassium chloride ＞processed products of calcium chloride ＞processed products of magnesium chloride. The order of effect/toxicity ratio was processed products of potassium chloride ＞processed products of magnesium chloride＞processed products of sodium chloride ＞processed products of calcium chloride ＞processed products of bittern ＞raw aconite. The order of toxicity component index was raw aconite ＞processed products of sodium chloride ＞processed products of bittern＞processed products of calcium chloride ＞processed products of potassium chloride ＞processed products of magnesium chloride. CONCLUSIONS ：Using 5 kinds of salt as excipients ，the proce ssing technology has different degrees of “efficacy enhancing and toxicity reducing ”effect. Among them ，magne- sium chloride ，potassium chloride and calcium chloride are better for processing and can be used for processing aconite. |
| 期刊： | 2020年第31卷第10期 |
| 作者： | 张存艳，刘红梅，魏蔼玲，袁明昊，郭力，陈胡兰，叶强 |
| AUTHORS： | ZHANG Cunyan ，LIU Hongmei ，WEI Ailing，YUAN Minghao ，GUO Li，CHEN Hulan，YE Qiang |
| 关键字： | 附子；盐制辅料；增效-减毒；高效液相色谱法；酯型生物碱；含量测定 |
| KEYWORDS： | Aconite；Salt-processed excipients ；Efficacy |
| 阅读数： | 604 次 |
| 本月下载数： | 5 次 |
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