甲泼尼龙琥珀酸钠对自身免疫性肺气肿模型大鼠氧化应激和抗内皮细胞抗体的影响
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篇名: 甲泼尼龙琥珀酸钠对自身免疫性肺气肿模型大鼠氧化应激和抗内皮细胞抗体的影响
TITLE: Effects of Methylprednisolone Sodium Succinate on Oxidative Stress and AECA of Autoimmune Emphysema Model Rats
摘要: 目的:研究甲泼尼龙琥珀酸钠对自身免疫性肺气肿模型大鼠氧化应激和抗内皮细胞抗体(AECA)的影响。方法:将雄性SD大鼠随机分为对照组、模型组和干预组,每组8只。除对照组大鼠腹腔注射等体积的完全弗氏佐剂外,模型组和干预组大鼠均腹腔注射体外培养的人脐静脉内皮细胞+完全弗氏佐剂混合物以复制自身免疫性肺气肿模型;造模给药后第2天,干预组大鼠腹腔注射甲泼尼龙琥珀酸钠10mg/(kg·d),对照组和模型组大鼠均腹腔注射等体积的生理盐水,每日1次,连续21d。末次给药后,取各组大鼠右肺组织制作石蜡切片并行苏木精-伊红染色,观察肺组织病理变化,测量单位面积内的肺泡数(MAN)和内衬间隔(MLI);检测左肺肺泡灌洗液(BALF)中丙二醛(MDA)、还原型谷胱甘肽(GSH)含量和超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性,以及BALF和血清中AECA含量。采用Pearson分析模型组大鼠BALF中AECA与MDA、GSH、SOD、GSH-Px的相关性。结果:与对照组比较,模型组大鼠肺组织可见明显的肺气肿病理变化,其MAN显著减少,MLI显著延长(P<0.01);BALF中GSH含量和SOD、GSH-Px活性均显著降低,BALF中MDA含量以及BALF、血清中AECA含量均显著升高(P<0.01)。与模型组比较,干预组大鼠肺组织的肺气肿病理变化明显改善,其MAN显著增加,MLI显著缩短(P<0.01);BALF中GSH含量和SOD、GSH-Px活性均显著升高;BALF中MDA含量以及BALF、血清中AECA含量均显著降低(P<0.01)。模型组大鼠BALF中的AECA与MDA呈正相关(r=0.710,P<0.05),与GSH、SOD、GSH-Px呈负相关(r=-0.754、-0.781、-0.736,P<0.05)。结论:甲泼尼龙琥珀酸钠可能通过减轻氧化应激反应、抑制AECA的表达,从而达到预防自身免疫性肺气肿的目的。
ABSTRACT: OBJECTIVE:To study the effects of methylprednisolone sodium succinate on oxidative stress and anti-endothelial cell antibody (AECA)of autoimmune emphysema model rats. METHODS :Male SD rats were randomly divided into control group,model group and intervention group ,with 8 rats in each group. Except that control group was given same volume of complete Freund ’s adjuvant intraperitoneally ,model group and intervention group were given the mixture of human umbilical vein endothelial cells and complete Freund ’s adjuvant intraperitoneally to establish the model of autoimmune emphysema. On 2nd day after modeling ,intervention group was intraperitoneally injected with 10 mg/(kg·d)methylprednisolone sodium succinate. Control group and model group were intraperitoneally injected the same volume of normal saline ,once a day ,for consecutive 21 days. After last medication ,the right lung tissue of rats were taken for paraffin section and HE staining in each group ,and the pathological changes of lung tissue were observed. The mean alveolar number (MAN)and mean linear intercept (MLI)were measured. The contents of MDA and GSH ,the activities of SOD and glutathione peroxidase (GSH-Px)in bronchial alveolar lavage fluid(BALF)of left lung were determined ;the contents of AECA in BALF and serum were also determined. The correlation of AECA with MDA ,GSH,SOD and GSH-Px were determined in model group by Pearson analysis. RESULTS :Compared with control group ,the pathological changes of pulmonary emphysema were obvious in model group ,MAN decreased significantly ,and MLI prolonged significantly (P<0.01); GSH content , GSH-Px and SOD activities in BALF were decreased 1100,1195) significantly,the contents of AECA in BALF and serum were increased significantly (P<0.01). Compared with modelgroup,the pathological changes of pulmonary emphysema was improved significantly in the intervention group , increased significantly ,while MLI shortened significantly (P< ·1208;GSH content ,SOD and GSH-Px activities were increased significantly,while the contents of AECA in BALF and serum were decreased significantly (P<0.01). AECA in BALF of rats in model group was positively correlated with MDA (r=0.710, P<0.05),and the AECA were negatively correlated with GSH ,SOD and GSH-Px (r=-0.754,-0.781,-0.736,P<0.05). CONCLUSIONS:Methylprednisolone sodium succinate may achieve the purpose of the prevention of autoimmune emphysema through reducing oxidative stress and inhibiting the expression of AECA.
期刊: 2020年第31卷第10期
作者: 李佳艺,张程,李本雪,张野,张湘燕
AUTHORS: LI Jiayi,ZHANG Cheng ,LI Benxue ,ZHANG Ye,ZHANG Xiangyan
关键字: 自身免疫性肺气肿;氧化应激;抗内皮细胞抗体;甲泼尼龙琥珀酸钠;大鼠
KEYWORDS: Autoimmune emphysema ; Oxidative stress ; Anti-endothelial cell antibodies ; Methylprednisolone sodium
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