干姜及其炮制品色差值与活性成分含量的相关性研究
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篇名: 干姜及其炮制品色差值与活性成分含量的相关性研究
TITLE: Correlation Study of Color Difference Values and Active Constituent Contents in Crude and Processed Zingiber officinale
摘要: 目的:评价干姜及其炮制品色差值与活性成分含量的相关性。方法:采用高效液相色谱法测定6种活性成分的含量,采用色彩色差计测定干姜及其炮制品的色差值[明度(L*)、红绿色轴分量(a*)、黄蓝色轴分量(b*)]。采用SPSS24.0软件对色差值与活性成分含量进行相关性分析。结果:姜酮、6-姜酚、8-姜酚、6-姜烯酚、二乙酰氧基-6-姜二醇和10-姜酚检测质量浓度的线性范围分别为2.65~105.90、10.15~406.00、4.87~194.80、5.28~211.20、6.14~245.70、7.02~280.80μg/mL(r均大于0.999);定量限分别为7.46、13.68、14.37、16.62、17.03、17.99ng,检测限分别为2.24、4.11、4.31、4.99、5.11、5.40ng;精密度、稳定性、重复性试验的RSD均小于3%;平均加样回收率分别为101.34%、102.14%、101.22%,103.12%、103.74%、103.54%,103.06%、98.55%、99.43%,99.36%、103.51%、101.21%,100.85%、99.42%、99.60%,100.39%、97.69%、103.84%(RSD均小于3%,n=3);含量分别为0~0.66、0.06~7.57、0.03~1.45、0.29~3.47、0.15~2.85、0.04~2.83mg/g。色差值L*、b*与干姜不同炮制程度呈极显著负相关(P<0.01),a*与炮制程度呈正相关(P<0.05);干姜炮制前后L*、b*与姜酮含量呈极显著负相关,与其余5种成分呈极显著正相关(P<0.01);a*与姜酮呈极显著正相关(P<0.01),与其余5种成分无相关性(P>0.05);干姜及其炮制品与姜酮含量呈极显著正相关,与其余5种成分含量呈极显著负相关(P<0.01)。结论:干姜及其炮制品的色差值与其活性成分含量相关,即随炮制程度的加重,其a*增加,L*、b*降低;姜酮含量升高,6-姜酚、8-姜酚、6-姜烯酚、二乙酰氧基-6-姜二醇、10-姜酚含量降低。
ABSTRACT: OBJECTIVE:To evaluate the correlation between color difference values and active constituent contents of crude and processed Zingiber officinale . METHODS :HPLC method was adopted to determint the content of 6 active constituents. The color difference values of crude and processed Z. officinale [lightness(L*),red-green axis component (a*),yellow-blue axis component(b*)] were determined by chromatic aberration meter . SPSS 24.0 software was adopted for the correlation analysis between color difference values and active constituent contents. RESULTS :The linear range of zingiberone ,6-gingerol, 8-gingerol, 6-shogaol, diacetoxy-6-gingerol and 10-gingerol were 2.65-105.90, 10.15-406.00, 4.87-194.80, 5.28-211.20, 6.14-245.70,7.02-280.80 μg/mL(r>0.999). The limits of quantification were 7.46,13.68,14.37,16.62,17.03,17.99 ng,and the limits of detection were 2.24,4.11,4.31,4.99,5.11,5.40 ng,respectively. RSDs of precision ,stability,and repeatability tests were all lower than 3%. The average recovery rates were 101.34%,102.14%,101.22%;103.12%,103.74%,103.54%;103.06%,properties critical for cell migration and invasion. induced EMT through downregulation of NF-κB-Snail sig- naling in breast cancer cells enchymal transition and activation of TLR 4/JNK signal - 98.55%,99.43%;99.36%,103.51%,101.21%;100.85%,99.42%,99.60%;100.39%,97.69%,103.84%(RSD were all lower than 3%,n=3),respectively. The contents of them were 0-0.66,0.06-7.57,0.03-1.45,0.29-3.47,0.15-2.85,0.04-2.83 mg/g, respectively. L* and b* values were negative correlated with the processing degree of Z. officinale significantly(P<0.01),a* showed a significantly positive correlation with the processing degree (P<0.05). L*and b* values showed a significantly negative correlation with the content of zingiberone before and after processing ,but positively correlated with the other five components (P<0.01). a* showed a significantly positive correlation with the content of zingiberone ,but had no correlation with other five components(P>0.05). The crude and processed Z. officinale were positive correlated with the content of zingiberone ,negatively correlated with other five components (P<0.01). CONCLUSIONS :There is a certain correlation between the color difference values of crude and processed Z. officinale and the contents of their active constituents. With the deepening of the processing ,a* values is increased ,L* values and b* values is decreased ;the content of zingiberone increases ,the contents of 6-gingerol, 8-gingerol,6-shogaol,diacetoxy-6-gingerol,10-gingerol reduce.
期刊: 2020年第31卷第10期
作者: 林华坚,张梓豪,孟江,王淑美
AUTHORS: LIN Huajian ,ZHANG Zihao,MENG Jiang,WANG Shumei
关键字: 干姜;炮制品;高效液相色谱法;色彩色差计;活性成分;含量;色差值;相关性分析
KEYWORDS: Crude Zingiber officinale ;Processed product ;HPLC;Chromatric aberration meter ;Active constituent ;Content;
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