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HPLC特征指纹图谱结合化学计量学评价不同寄主来源的桑寄生药材质量
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| 篇名： | HPLC特征指纹图谱结合化学计量学评价不同寄主来源的桑寄生药材质量 |
| TITLE： | Evaluation of the Quality of Taxillus chiueusis from Different Hosts Sources by HPLC Fingerprint Combined with Chemometrics |
| 摘要： | 目的：评价不同寄主来源的桑寄生药材质量，建立具有寄主针对性的桑寄生药材质量评价方法。方法：采用高效液相色谱（HPLC）法测定，色谱柱为CAPCELLPAKC18MGⅡ，流动相为甲醇-0.2％磷酸水溶液，流速为1.0mL/min（梯度洗脱），测定波长为254nm，柱温为30℃。以16批不同寄主来源的桑寄生药材为样品（桑树寄主、柳树寄主、茶树寄主、枫香树寄主的桑寄生各4批），采用《中药色谱指纹图谱相似度评价系统》（2004A版）生成HPLC特征图谱并进行相似度评价，再通过与对照品比对进行色谱峰的指认。采用IBMSPSS19.0软件对结果进行聚类分析和主成分分析。结果：从建立的共有特征图谱中标定出了11个共有峰，并指认了8、10、11号峰分别为芦丁、槲皮苷和槲皮素；16批样品的相似度均大于0.9，不同寄主来源的桑寄生在化学成分种类上基本一致（各共有峰相对保留时间的RSD为0.03％～0.40％），但同一成分的含量差异较大（各共有峰相对峰面积的RSD为27.00％～64.20％）。聚类分析将16批不同寄主来源的桑寄生分成3类；主成分分析结果提示，槲皮苷、槲皮素和芦丁对桑寄生品质有明显的影响，可作为桑寄生质量评价指标，且枫香树寄主桑寄生样品的整体质量最好（综合评分最高）。结论：HPLC特征指纹图谱结合化学计量的方法可作为不同寄主来源的桑寄生药材质量评价和控制的技术方法。 |
| ABSTRACT： | OBJECTIVE：To establish a host specific method for the qual ity evaluation of Taxillus chiueusis by evaluating the quality of T. chiueusis from different hosts sources. METHODS ：HPLC was adopted with CAPCELL PAK C 18 MGⅡ column， mobile phase consisted of methanol- 0.2％ phosphoric acid ，volume flow 1.0 mL/min（gradient elution ），detection wavelength of 254 nm，column temperature of 30 ℃. Totally of 16 batches of T. chiueusis from different hosts sources were collected as sample （4 batches from tea host ，maple host ，willow host and mulberry host respectively ）. HPLC characteristic chromatogram was established with TCM Chromatogram Fingerprint Similarity Evaluation System （2004A edition ），and similarity evaluation was performed. The peak was identified by comparison with the reference substance. IBM SPSS 19.0 software was used for cluster analysis and principal component analysis. RESULTS ：Totally 11 common peaks were demarcated ，peak No. 8，10，11 were identified as rutin ，quercetin and quercitin. The similarity of 16 batches of samples was more than 0.9. The chemical components of T. chiueusis from different hosts sources were basically the same （RSDs of relative time of commom peaks were 0.03％-0.40％）， but the contents of the same component were quite different （RSDs of relative peak area of commom peaks were 27.00％-64.20％）. By cluster analysis ，16 batches of T. chiueusis from different hosts sources were divided into 3 types. The results of principle component analysis showed that quercetin ，quercitin and rutin had significant effect on the quality of T. chiueusis ，which could be used as the quality evaluation index ，and the whole quality of maple host was the best （the highest comprehensive score ）. CONCLUSIONS： HPLC fingerprint combined with chemometrics can be used to evaluate the quality of T. chiueusis from different hosts sources. |
| 期刊： | 2020年第31卷第07期 |
| 作者： | 卢森华，甘洋萦，唐莲，张凤懿，苏本伟 |
| AUTHORS： | LU Senhua ，GAN Yangying ，TANG Lian，ZHANG Fengyi ，SU Benwei |
| 关键字： | 桑寄生；不同寄主；高效液相色谱法；指纹图谱；聚类分析；主成分分析；质量评价 |
| KEYWORDS： | Taxillus chiueusis ；Different hosts ；HPLC； |
| 阅读数： | 298 次 |
| 本月下载数： | 13 次 |
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