红花保存年限、有效成分含量和颜色值的相关性分析
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篇名: 红花保存年限、有效成分含量和颜色值的相关性分析
TITLE: Correlation Analysis of Storage Life and Effective Composition Content with Color Value of Carthamus tinctorius
摘要: 目的:考察红花保存年限、有效成分含量与颜色值的相关性,为不同保存年限的红花药材质量评价提供参考。方法:以来自同一产地的不同保存年限(0、1、2年,各8批)的24批红花药材为样品,采用高效液相色谱法测定其羟基红花黄色素A(HSYA)和山柰酚的含量,采用分光测色仪测定其粉末颜色值[明暗程度值(L*)、红绿色色调值(a*)、黄蓝色色调值(b*)],并应用SPSS19.0统计软件对其保存年限、有效成分含量和颜色值进行相关性分析。结果:保存1年或2年的药材中山柰酚含量仍较高(分别为0.161%、0.061%),但药材中HSYA含量随着保存年限的延长而降低(保存0、1、2年的药材中HSYA平均含量分别为2.46%、1.58%、1.51%),且药材颜色变暗(a*值降低)。相关分析结果显示,HSYA含量与颜色值L*、a*呈显著正相关(r=0.430、0.781,P<0.05或P<0.01),HSYA含量与保存年限呈显著负相关(r=-0.777,P<0.01),其余各变量间无相关性(P>0.05)。结论:保存年限越长,红花药材颜色越深、HSYA含量越低,故该药材不宜过年或多年保存。
ABSTRACT: OBJECTIVE:To investigate the correlation of storage life and effective composition content with color value of Carthamus tinctorius ,and to provide reference for the quality evaluation of C. tinctorius with different years of storage. METHODS:Using 24 batches of C. tinctorius from same place of production with different years of storage (0,1,2 years,8 batches each type )as samples ,the contents of hydroxysafflor yellow A (HSYA)and kaempferol were determined by HPLC. Color value [lightness value (L*),red-green value (a*),yellow-blue value (b*)] were determined by spectrophotometer. SPSS 19.0 statistical software was used to analyze the correlation of storage life and effective composition content with color value. RESULTS:Kaempferol content was still high after 1 year or 2 years of storage (0.161%,0.061%,respectively). However ,the content of HSYA decreased with the prolongation of the storage life (the average content of HSYA were 2.46%,1.58%,and 1.51% after storage 0,1 and 2 years,respectively),and the color of the drug became darker (a* value decreased ). Results of correlation analysis showed that the content of HSYA was positively associated with color value L*,a*(r=0.430,0.781,P<0.05 or P<0.01);the content of HSAY was negatively associated with storage life (r=-0.777,P<0.01). There was no correlation between the remaining variables (P>0.05). CONCLUSIONS :The longer the storage life ,the darker the color and the lower the content of HSYA ,so it is not suitable for over year and multiyear preservation.
期刊: 2020年第31卷第05期
作者: 王洁,苏少锋,姚仁川,任超翔,裴瑾,陈江
AUTHORS: WANG Jie,SU Shaofeng,YAO Renchuan,REN Chaoxiang,PEI Jin,CHEN Jiang
关键字: 红花;保存年限;羟基红花黄色素A;山柰酚;色泽;品质;相关性分析
KEYWORDS: Carthamus tinctorius ;Storage life ;Hydroxysafflor yellow A ;Kaempferol;Color;Quality;Correlation analysis
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