基于大鼠离体外翻肠囊模型研究不同粒径天麻粉的吸收特性
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篇名: 基于大鼠离体外翻肠囊模型研究不同粒径天麻粉的吸收特性
TITLE: Study on Absorption Characteristics of Gastrodia elata Powder with Different Particle Sizes Based on Rat Everted Intestinal Sac Model in vitro
摘要: 目的:比较不同粒径天麻粉中天麻素、巴利森苷A、巴利森苷B、巴利森苷C的肠吸收特征,探讨粒径对上述成分肠吸收的影响。方法:采用大鼠外翻肠囊模型,以累积吸收量(Q)和吸收速率常数(Ka)为指标,采用超高效液相色谱-串联质谱法测定不同剂量(2.5、5、10g/L)和不同粒径(细粉146μm、极细粉52μm、超微粉37μm)的天麻粉中天麻素、巴利森苷A、巴利森苷B、巴利森苷C在不同肠段(十二指肠、空肠、回肠、结肠)中的吸收情况。结果:2.5g/L天麻极细粉中天麻素和巴利森苷B的Q、Ka值(全肠段),巴利森苷C的Q值(结肠)和Ka值(回肠、结肠);2.5g/L天麻超微粉中天麻素的Q、Ka值(全肠段),巴利森苷B的Q、Ka值(十二指肠、空肠、回肠),巴利森苷C的Ka值(结肠);5g/L天麻极细粉中天麻素的Q值(十二指肠),巴利森苷A和巴利森苷B的Q值(全肠段),巴利森苷C的Q值(十二指肠、空肠);5g/L天麻超微粉中天麻素的Q值(十二指肠、空肠、结肠)和Ka值(全肠段),巴利森苷B的Q值(十二指肠、回肠、结肠),巴利森苷C的Q值(十二指肠、回肠);10g/L天麻极细粉中巴利森苷B的Q、Ka值(空肠、回肠),巴利森苷C的Q值(空肠、回肠)以及10g/L天麻超微粉中天麻素的Q值(结肠)和Ka值(十二指肠),巴利森苷B的Q值(十二指肠、回肠、结肠)和Ka值(十二指肠、结肠),巴利森苷C的Q值(十二指肠、回肠)和Ka值(十二指肠)均较同剂量天麻细粉显著升高(P<0.05或P<0.01)。2.5g/L天麻极细粉中巴利森苷A的Ka值(空肠),巴利森苷C的Q值(十二指肠);2.5g/L天麻超微粉中巴利森苷A的Ka值(空肠、回肠),巴利森苷C的Q、Ka值(十二指肠、空肠);5g/L天麻极细粉中天麻素的Ka值(空肠、回肠、结肠),巴利森苷A的Ka值(结肠),巴利森苷B的Ka值(回肠),巴利森苷C的Ka值(空肠、回肠);5g/L天麻超微粉中天麻素和巴利森苷C的Ka值(空肠、回肠、结肠),巴利森苷A的Q值(空肠、结肠)和Ka值(结肠),巴利森苷B的Ka值(空肠、回肠);10g/L天麻极细粉中巴利森苷A的Q、Ka值(回肠);10g/L天麻超微粉中巴利森苷A的Q值(十二指肠)和Ka值(空肠),巴利森苷C的Ka值(空肠)均较同剂量天麻细粉显著降低(P<0.05或P<0.01)。2.5g/L天麻细粉中天麻素的Q值(结肠),巴利森苷A的Q值(结肠)和Ka值(回肠、结肠),巴利森苷B的Q、Ka值(空肠、结肠),巴利森苷C的Q、Ka值(回肠、结肠);2.5g/L天麻极细粉中天麻素的Q、Ka值(结肠),巴利森苷A的Q值(回肠、结肠)和Ka值(空肠、回肠、结肠),巴利森苷C的Ka值(结肠);2.5g/L天麻超微粉中巴利森苷A和巴利森苷C的Q值(结肠)和Ka值(空肠、回肠、结肠),巴利森苷B的Q、Ka值(回肠、结肠);5g/L天麻细粉中天麻素、巴利森苷A和巴利森苷C的Q、Ka值(结肠),巴利森苷B的Ka值(结肠);5g/L天麻极细粉中天麻素和巴利森苷A的Q、Ka值(结肠),巴利森苷C的Q、Ka值(空肠、回肠、结肠);5g/L天麻超微粉中天麻素的Q、Ka值(回肠、结肠),巴利森苷A的Q值(空肠、回肠、结肠),巴利森苷B的Q、Ka值(空肠、结肠),巴利森苷C的Q值(空肠、结肠)和Ka值(空肠、回肠、结肠);10g/L天麻细粉中天麻素的Q值(结肠)以及巴利森苷A、巴利森苷B、巴利􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂肌缺血再灌注损伤后心肌细胞凋亡的影响[J].中华中医[17]严家荣,梁志健,王弋,等.两种高脂高糖乳脂配方复制高药学刊,2019,37(6):1380-1384.脂血症大鼠模型的比较[J].动物医学进展,2017,38[15]余雪,余恒,周涛,等.缺血预适应对脑梗死患者疗效及血(10):38-42.清凋亡相关因子水平的影响[J].疑难病杂志,2019,18[18]PATELJV,CASLAKEMJ,VYASA,etal.Triglucerides(5):446-449.andsmalldenselowdensitylipoproteininthediscrimina-[16]智凤,徐日新.改善冠心病患者低密度脂蛋白胆固醇的研tionofcoronaryheartdiseaseriskinsouthAsianpopula-究进展[J].实用临床医药杂志,2018,22(24):137-140.tions[J].Atherosclerosis,2010,209(2):579-584.[19]朱艳彬,朱伟群.HDL-C与老年冠心病患者临床预后的Δ基金项目:国家自然科学基金资助项目(No.81560646,No.关联性分析[J].重庆医学,2018,47(33):4260-4262.U1812403);中央引导地方科技发展专项资金项目(No.黔科中引地[20]XIEJ,LIB,YAOB,etal.Transforminggrowthfactor-β1-〔2018〕4006);贵州省科技计划项目(No.黔科合平台人才〔2016〕5613,No.黔科合平台人才〔2016〕5677);贵阳市科技计划项目(No.筑科合同regulatedFAS/FASLpathwayactivationsuppressesnucle-〔2017〕30-29号)uspulposuscellapoptosisinaninflammatoryenviron-*硕士研究生。研究方向:中药新药研发及质量控制。E-mail:ment[J].BioscienceRep,2019.DOI:10.1042/BSR201917-1924855171@qq.com26.#通信作者:教授,硕士生导师。研究方向:中药新药研发。电(收稿日期:2019-07-24修回日期:2019-12-29)话:0851-86908468。E-mail:gywam100@163.com(编辑:段思怡)中国药房2020年第31卷第4期ChinaPharmacy2020Vol.31No.4·413·森苷C的Q、Ka值(空肠、回肠、结肠);10g/L天麻极细粉中天麻素的Q值(结肠),巴利森苷A和巴利森苷C的Q、Ka值(空肠、回肠、结肠),巴利森苷B的Q、Ka值(结肠);10g/L天麻超微粉中天麻素的Q值(结肠)和Ka值(空肠、回肠、结肠),巴利森苷A和巴利森苷C的Q、Ka值(空肠、回肠、结肠),巴利森苷B的Q值(空肠、回肠、结肠)和Ka值(回肠、结肠)均较同组十二指肠显著降低(P<0.05)。2.5g/L天麻极细粉中天麻素的Q、Ka值(空肠),2.5g/L天麻超微粉中天麻素的Q值(空肠、回肠)和Ka值(空肠),5g/L天麻细粉中天麻素的Q、Ka值(空肠、回肠);2.5g/L天麻极细粉中巴利森苷B的Q值(空肠、回肠)和Ka值(空肠),5g/L天麻细粉中巴利森苷B的Ka值(空肠、回肠),10g/L天麻极细粉中巴利森苷B的Ka值(回肠)均较同组十二指肠显著升高(P<0.05)。5g/L以及10g/L天麻细粉、极细粉、超微粉中天麻素、巴利森苷A、巴利森苷B、巴利森苷C的Q、Ka值(全肠段)均较同肠段同粒径2.5g/L天麻粉显著升高(P<0.05或P<0.01)。结论:天麻中的4种有效成分在4个肠段均有吸收,且主要集中于小肠。天麻中的天麻素可能为被动吸收,巴利森苷类则可能为主动转运;粒径可影响上述4种有效成分的肠吸收特性。
ABSTRACT: OBJECTIVE:To compar e the absorpt ion characteristics of gastrodin ,parishin A ,parishin B and parishin C of Gastrodia elata powder,and to explore the effect of particle size on intestinal absorption of above components. METHODS :Based on everted intestinal sac model ,using accumulative absorption amount (Q)and absorption rate constant (Ka)as indexes ,UPLC-MS/MS method was used to determine the absorption of gastrodin ,parishin A ,parishin B and parishin C from different doses (2.5,5,10 g/L) of G. elata powder with different particle sizes (fine powder 146 μm,superfine powder 52 μm,ultrafine powder 37 μm)in different segments(duodenum,jejunum,ileum and colon ). RESULTS :Q and Ka of gastrodin and parishin B (intestinal segment ),Q(colon) and Ka(ileum and colon )of parishin C in 2.5 g/L G. elata superfine powder ;Q and Ka of gastrodin (intestinal segment ),Q and Ka of parishin B (duodenum,jejunum,ileum)and Ka of parishin C (colon)in 2.5 g/L G. elata ultrafine powder ;Q of gastrodin (duodenum),Q of parishin A and parishin B (intestinal segment )and Q of parishin C (duodenum,jejunum)in 5 g/L G. elata superfine powder ;Q(duodenum jejunum ,colon)and Ka(intestinal segment )of gastrodin ,Q of parishin B (duodenum,ileum and colon)and Q of parishin C (duodenum,ileum)in 5 g/L G. elata ultrafine powder ;Q and Ka of parishin B (jejunum,ileum),Q of parishin C (jejunum,ileum)in 10 g/L G. elata superfine powder as well as Q(colon)and Ka(duodenum)of gastrodin ,Q (duodenum,ileum,colon)and Ka(duodenum,colon)of parishin B ,Q(duodenum,ileum)and Ka(duodenum)of parishin C in 10 g/L G. elata ultrafine powder were all increased significantly ,compared with the same dose of G. elata fine powder (P<0.05 or P<0.01). Ka of parishin A (jejunum)and Q of parishin C (duodenum)in 2.5 g/L G. elata superfine powder ;Ka of parishin A (jejunum,ileum), Q and Ka of parishin C (duodenum,jejunum)in 2.5 g/L G. elata ultrafine powder ;Ka of gastrodin (jejunum,ileum and colon ),Ka of parishin A (colon),Ka of parishin B (ileum)and Ka of parishin C (jejunum,ileum)in 5 g/L G. elata superfine powder ;Ka of gastrodin and parishin C (jejunum,ileum and colon ),Q(jejunum,colon)and Ka(colon)of parishin A ,Ka of parishin B (jejunum,ileum)in 5 g/L G. elata ultrafine powder ;Q and Ka of parishin A (ileum)in 10 g/L G. elata superfine powder ;Q(duodenum)and Ka(jejunum) of parishin A ,Ka of parishin C (jejunum)in 10 g/L G. elata ultrafine powder were decreased significantly ,compared with the same dose of G. elata fine powder (P<0.05 or P<0.01). Q of gastrodin (colon),Q(colon)and Ka(ileum,colon)of parishin A ,Q and Ka of parishin B (jejunum,colon),Q and Ka of parishin C (ileum,colon)in 2.5 g/L G. elata fine powder ;Q and Ka of gastrodin (colon),Q(ileum,colon)and Ka(jejunum,ileum,colon)of parishin A ,Ka of parishin C (colon)in 2.5 g/L G. elata superfine powder;Q(colon)and Ka(jejunum,ileum,colon)of parishin A and C ,Q and Ka(ileum,colon)of parishin B in 2.5 g/L G. elata ultrafine powder ;Q and Ka of gastrodin ,parishin A and C (colon),Ka of parishin B (colon)in 5 g/L G. elata fine powder ;Q and Ka of gastrodin and parishin A (colon),Q and Ka of parishin C (jejunum,ileum,colon)in 5 g/L G. elata superfine powder ;Q and Ka of gastrodin(ileum,colon),Q of parishin A (jejunum,ileum,colon),Q and Ka of parishin B (jejunum,colon),Q(jejunum,colon) and Ka(jejunum,ileum,colon)of parishin C in 5 g/L G. elata ultrafine powder ;Q of gastrodin (colon),Q and Ka of parishin A ,B and C (jejunum,ileum,colon)in 10 g/L G. el ata fine powder ;Q of gastrodin (colon),Q and Ka of parishin A and C (jejunum, ileum,colon),Q and Ka of parishin B (colon)in 10 g/L G. elata superfine powder ;Q(colon)and Ka(jejunum,ileum,colon)of gastrodin,Q and Ka of parishin A and C (jejunum,ileum,colon),Q(jejunum,ileum,colon)and Ka(ileum,colon)of parishin B in 10 g/L G. elata ultrafine powder were decreased significantly ,compared with duodeum of the same group (P<0.05). Q and Ka of gastrodin(jejunum)in 2.5 g/L G. elata superfine pow
期刊: 2020年第31卷第04期
作者: 陈艳,刘帆,巩仔鹏,陈亭亭,陶陶,刘智,王爱民
AUTHORS: CHEN Yan,LIU Fan,GONG Zipeng ,CHEN Tingting ,TAO Tao,LIU Zhi,WANG Aimin
关键字: 天麻;粒径;外翻肠囊模型;吸收特性;天麻素;巴利森苷类
KEYWORDS: Gastrodia elata ;Particle size ;Everted intestinal sac model ;Absorption characteristics ;Gastrodin;Balisensides
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