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蒙药苏格木勒-3汤不同部位中4种成分的含量测定
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| 篇名： | 蒙药苏格木勒-3汤不同部位中4种成分的含量测定 |
| TITLE： | Content Determination of 4 Components in Different Parts of Mongolian Medicine Sugmel- 3 Decoction |
| 摘要： | 目的：建立测定蒙药苏格木勒-3汤水提物中芹菜素、胡椒碱及其挥发油中桉油精、枯茗醛含量的方法。方法：采用高效液相色谱法（HPLC）测定芹菜素、胡椒碱的含量；采用气相色谱法（GC）测定桉油精、枯茗醛的含量。HPLC法采用色谱柱为AgilentEclipseXDB-C18，流动相为甲醇-0.1％磷酸水溶液，流速为1.0mL/min，检测波长为225（芹菜素）、342nm（胡椒碱），柱温为30℃，进样量为10μL。GC法采用色谱柱为DimensionsSH-Rtx-1毛细管柱，载气为高纯氢气，进样口温度为270℃，载气流量为1mL/min，程序升温，进样量为10μL，进样分流比为1∶10。结果：芹菜素、胡椒碱、桉油精、枯茗醛检测质量浓度的线性范围分别为12.5～200μg/mL（r＝0.9996）、87.3～139.7μg/mL（r＝0.9999）、136～2187μg/mL（r＝0.9999）、39～635μg/mL（r＝0.9999）；定量限分别为0.02、0.06、0.06、0.12μg/mL；检测限分别为0.01、0.02、0.03、0.04μg/mL；精密度、稳定性、重复性试验的RSD均小于4％；加样回收率分别为89.26％～97.26％（RSD＝2.69％，n＝6）、94.20％～104.01％（RSD＝3.64％，n＝6）、98.51％～110.11％（RSD＝3.87％，n＝6）、95.76％～107.82％（RSD＝4.12％，n＝6）；含量分别为0.769～0.828、7.741～7.981、5.2847～5.8466、1.0386～1.1012mg/g（n＝3）。结论：所建方法简单、可行，可用于蒙药苏格木勒-3汤不同部位的质量控制。 |
| ABSTRACT： | OBJECTIVE：To establish a method for the content determination of apigenin and piperine in the water extract as well as eucalyptol and cumin aldehyde in the volatile oil of Mongolian medicine Sugmel- 3 decoction. METHODS ：HPLC method was adopted for the content determination of apigenin and piperine. GC method was used for the content determination of eucalyptol and cumin aldehyde. The determination of HPLC method was performed on Agilent Eclipse XDB-C 18 column with mobile phase consisted of methanol- 0.1％ phosphoric acid aqueous solution at flow rate of 1.0 mL/min；the detection wavelength was set at 225 nm（apigenin）and 342 nm（piperine）；the column temperature was set at 30 ℃ with sample size of 10 μL. The determination of GC method was performed on Dimensions SH-Rtx- 1 capillary column with high-purity hydrogen as carrier gas ； the injector temperature was set at 270 ℃，with flow rate of carrier gas 1 mL/min by temperature programmed ；the sample size was 1 μL，and split ratio was 1 ∶ 10. RESULTS：The linear ranges of apigenin ，piperine，eucalyptol and cumin aldehyde were 12.5-200 μg/g/mL（r＝0.999 6），87.3-139.7 μg/mL（r＝0.999 9），136-2 187 μg/mL（r＝0.999 9），39-635 μg/mL（r＝0.999 9）， respectively. The quantitation limits were 0.02，0.06，0.06，0.12 μg/mL，respectively. The detection limits were 0.01，0.02，0.03， 0.04 μg/mL. RSDs of precision，stability and reproducibility tests were all less than 4％. The recovery rates of the samples were 89.26％ -97.26％（RSD＝2.69％ ，n＝6），94.20％ -104.01％（RSD＝3.64％ ，n＝6），98.51％ -110.11％（RSD＝3.87％ ，n＝6）， 95.76％-107.82％（RSD＝4.12％，n＝6），respectively. The contents of above components were 0.769-0.828，7.741-7.981，5.284 7- 5.846 6，1.038 6-1.101 2 mg/g（n＝3）. CONCLUSIONS：The established method is simple and feasible ，and can be used for quality control of different parts of Mongolian medicine Sugmel- 3 decoction. |
| 期刊： | 2020年第31卷第02期 |
| 作者： | 王树梅，韩金美，乌日罕，萨础拉 |
| AUTHORS： | WANG Shumei ，HAN Jinmei ，Wurihan，Sachula |
| 关键字： | 高效液相色谱法；气相色谱法；苏格木勒-3汤；水提物；挥发油；芹菜素；胡椒碱；桉油精；枯茗醛；含量测定 |
| KEYWORDS： | HPLC；GC；Sugmel-3 decoction；Water extract ；Volatile oil ；Apigenin；Piperine；Eucalyptol；Cumin aldehyde ； |
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