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基于炎症学说探讨迎春花总黄酮对T2DM模型小鼠胰岛功能的影响及可能机制
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| 篇名： | 基于炎症学说探讨迎春花总黄酮对T2DM模型小鼠胰岛功能的影响及可能机制 |
| TITLE： | |
| 摘要： | 目的：探讨迎春花总黄酮（TFJN）对2型糖尿病（T2DM）模型小鼠胰岛功能的影响及可能机制。方法：取昆明种小鼠10只作为空白组，以普通饲料喂养；其余75只小鼠作为造模组，以高脂高糖饲料喂养4周结合单次舌下静脉注射链脲佐菌素以复制T2DM模型。取造模成功小鼠，随机分为模型组、盐酸二甲双胍组（阳性对照，350 mg/kg）和TFJN低、中、高剂量组（100、200、400 mg/kg，以生药量计），每组10只。空白组和模型组小鼠均灌胃等体积生理盐水，各给药组小鼠均灌胃相应药物，每日1次，连续6周。分别于造模后（给药前）和给药后2、4、6周检测各组小鼠的空腹动态血糖水平，采用苏木精-伊红染色法观察其胰腺组织形态学特征，采用酶联免疫吸附测定法检测其血清胰岛素（INS）、胰岛素自身抗体（IAA）、肿瘤坏死因子α（TNF-α）、白细胞介素1β（IL-1β）、IL-6、IL-18、C反应蛋白（CRP）、瘦素（LP）、脂联素（ADPN）含量。结果：与空白组比较，模型组小鼠胰腺组织中胰岛萎缩、边界模糊，胞浆不丰富且体积变小；其空腹动态血糖水平（给药后各时间点）、IAA、TNF-α、IL-1β、IL-6、IL-18、CRP、LP含量均显著升高，INS、ADPN含量均显著降低（P＜0.01）。与模型组比较，各给药组小鼠胰腺组织上述症状均有所减轻；其空腹动态血糖水平（盐酸二甲双胍组给药后2、4、6周，TFJN各剂量组给药后4、6周）、IAA、TNF-α、IL-1β、IL-6、IL-18、CRP、LP含量均显著降低，INS、ADPN含量均显著升高（P＜0.05或P＜0.01）。结论：TFJN可修复T2DM模型小鼠的受损胰腺组织、降低其血糖水平、提高胰岛素敏感性，其作用机制可能与减少小鼠体内炎症因子的分泌有关。 |
| ABSTRACT： | OBJECTIVE： To investigate the effects of total flavonoids of Jasminum nudiflorum （TFJN） on pancreatic islet function in type 2 diabetes mellitus （T2DM） model mice and its possible mechanism. METHODS： Totally 10 Kunming mice were included in blank group and given normal diet. Other 75 mice were included in model group and given high-lipid and high-glucose diet for 4 weeks combined with streptozotocin once via sublingual vein to induce T2DM model. After modeling， the mice were randomly divided into model group， metformin hydrochloride group （positive control， 350 mg/kg）， TFJN low-dose， medium-dose and high-dose groups （100， 200， 400 mg/kg， by crude drug）， with 10 mice in each group. Blank group and model group were given constant volume of normal saline intragastrically； administration groups were given relevant medicine intragastrically； once a day， for consecutive 6 weeks. The level of fasting dynamic blood glucose was measured after modeling （before medication） and 2， 4， 6 weeks after medication. The histomorphological characteristics of pancreatic tissue were observed by HE staining. The contents of serum insulin （INS）， insulin cell antibody （IAA）， TNF-α， IL-1β， IL-6， IL-18， CRP， leptin （LP） and adiponectin （ADPN） were measured by ELISA. RESULTS： Compared with blank group， islet atrophy， blurred boundary， insufficient cytoplasm and smaller volume of pancreatic tissue were observed in model group. The fasting dynamic blood glucose level （at different time points after medication）， the contents of IAA， TNF-α， IL-1β， IL-6， IL-18， CRP and LP were increased significantly， while the contents of INS and ADPN were decreased significantly （P＜0.01）. Compared with model group， above symptoms of pancreatic tissue in administration groups were relieved， and the fasting dynamic blood glucose level （2， 4， 6 weeks after administration in metform in hydrochloride group， 4， 6 weeks after administration in TFJN groups）， the contents of IAA， TNF-α， IL-1β， IL-6， IL-18， CRP and LP were decreased signifi- cantly， while the contents of INS and ADPN were increased significantly （P＜0.05 or P＜0.01）. CONCLUSIONS： TFJN can repair the damaged pancreas of mice with T2DM， reduce the level of blood glucose， and improve the sensitivity of the body to insulin， the mechanism of which may be associated with reducing the secretion of inflammatory factors. |
| 期刊： | 2019年第30卷第22期 |
| 作者： | 陈晓萌，范田，孙俊，马培志 |
| AUTHORS： | CHEN Xiaomeng，FAN Tian，SUN Jun，MA Peizhi |
| 关键字： | 迎春花；总黄酮；2型糖尿病；血糖；胰岛素；炎症因子；机制；小鼠 |
| KEYWORDS： | Jasminum nudiflorum； Total flavonoids； Type 2 diabetes mellitus； Blood glucose； Insulin； Inflammatory factor； Mechanism； Mice |
| 阅读数： | 169 次 |
| 本月下载数： | 6 次 |
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