氧化槐果碱对离体大鼠胸主动脉环的舒张作用及其机制研究
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篇名: 氧化槐果碱对离体大鼠胸主动脉环的舒张作用及其机制研究
TITLE:
摘要: 目的:研究氧化槐果碱(OSC)对离体大鼠胸主动脉环的舒张作用及其机制。方法:取大鼠胸主动脉环(简称“血管环”),以舒张率为指标,以K-H营养液为空白对照,分别考察不同质量浓度的OSC(0.2~1.0 mg/mL)对基础状态的正常血管环,以及经去甲肾上腺素(PE,1×10-6 mol/L)预收缩的正常或去内皮血管环的舒张作用;分别以一氧化氮合酶抑制剂L-硝基精氨酸甲酯(L-NAME)、环氧合酶抑制剂吲哚美辛(INDO)预孵育大鼠正常胸主动脉环,以4种钾通道阻滞剂[氯化钡(BaCl2)、四乙基胺(TEA)、4-氨基吡啶(4-AP)、格列本脲(Gli)]预孵育去内皮血管环,同法考察不同质量浓度的OSC(0.2~1.0 mg/mL)对上述血管环的舒张作用。结果:与空白对照比较,不同质量浓度的OSC对基础状态的正常血管环的舒张率无显著影响(P>0.05),但0.4~1.0 mg/mL的OSC能显著提高经PE预收缩的正常或去内皮血管环的舒张率(P<0.01),且呈浓度依赖趋势。经L-NAME、INDO、4-AP、BaCl2预孵育后,不同质量浓度的OSC对经PE预收缩的正常或去内皮血管环的舒张率均无显著影响(P>0.05);而经TEA、Gli预孵育后,0.4~1.0 mg/mL的OSC可显著降低经PE预收缩的去内皮血管环的舒张率(P<0.01)。结论:OSC在试验剂量(0.2~1.0 mg/mL)范围内对基础状态的大鼠胸主动脉环无明显舒张作用,但0.4~1.0 mg/mL的OSC对经PE预收缩的正常或去内皮大鼠胸主动脉环均有明显舒张作用;其血管舒张的作用机制为非内皮依赖性,可能与受体操纵性钙通道、钙激活钾通道和ATP敏感钾通道有关。
ABSTRACT: OBJECTIVE: To study the vasodilatory effect of oxysophocarpine (OSC) on isolated thoracic aortic rings of rats and its possible mechanism. METHODS: Thoracic aortic rings of rats were collected (called “vascular ring” for short). Using K-H nutrient solution as blank control and the diastolic rate as index, the effects of different concentrations (0.2-1.0 mg/mL) of OSC on normal vascular rings in basal state, normal or endothelium-free vascular rings pre-contracted by norepinephrine (PE, 1×10-6 mol/L) were investigated. After pre-culturing normal thoracic aortic rings by nitric oxide synthase inhibitor L-nitro-arginine methyl ester(L-NAME)and cyclooxygenase inhibitor indomethacin(INDO),as well as pre-culturing endothelium-free vascular rings by potassium ion channel blocker BaCl2,tetraethylammonium(TEA)and 4-aminopyridine(4-AP), the diastolic effects of OSC of different concentrations (0.2-1.0 mg/mL) on the above vascular rings were investigated by using the same method. RESULTS: Compared with blank control, there was no significant effects of different concentrations of OSC on the diastolic rate of normal vascular rings in basal state (P>0.05), but 0.4-1.0 mg/mL OSC could significantly improve the diastolic rate of normal or endothelium-free vascular rings pre-contracted by PE (P<0.01), in concentration-dependent manner. After preculturing with L-NAME, INDO, 4-AP and BaCl2, different concentrations of OSC had no significant effect on the diastolic rate of normal or endothelium-free vascular rings pre-contracted by PE (P>0.05). After pre-culturing with TEA and Gli, 0.4-1.0 mg/mL OSC could significantly reduce the diastolic rate of endothelium-free vas- cular rings pre-contracted by PE (P<0.01). CONCLUSIONS: OSC did not significantly dilate the thoracic aortic rings of rats in the basal state within the dose range (0.2-1.0 mg/mL), but OSC of 0.4-1.0 mg/mL have significant diastolic effects on the normal or endothelium-free thoracic aortic rings of rats pre-contracted with PE. The mechanism of thoracic aortic rings dilation is endothelium-independent, which may be associated with receptor operational calcium channel,Ca2+-activated potassium channels and ATP-sensitive potassium channels.
期刊: 2019年第30卷第22期
作者: 乔海琦,闫琳,余洋,常智,王佳玲,裴延敏,周茹
AUTHORS: QIAO Haiqi,YAN Lin,YU Yang,CHANG Zhi,WANG Jialing,PEI Yanmin,ZHOU Ru
关键字: 氧化槐果碱;胸主动脉环;血管舒张;内皮依赖性;钾通道;钙通道;机制
KEYWORDS: Oxysophocarpine; Thoracic aortic rings; Vasodilation; Endothelium independent;Potassium channel; Calcium channel; Mechanism
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