GC法同时测定艾纳香油中6种成分的含量
x

请在关注微信后,向客服人员索取文件

篇名: GC法同时测定艾纳香油中6种成分的含量
TITLE:
摘要: 目的:建立同时测定艾纳香油中β-蒎烯、芳樟醇、L-樟脑、L-龙脑、β-石竹烯、花椒油素含量的方法。方法:采用气相色谱法。色谱柱为RTX-1701毛细管柱,程序升温,检测器为氢离子火焰检测器,检测器温度为240 ℃,进样口温度为240 ℃,载气为高纯氮气,流速为3 mL/min,进样量为0.5 μL,进样分流比为50 ∶ 1。结果:β-蒎烯、芳樟醇、L-樟脑、L-龙脑、β-石竹烯、花椒油素的检测质量浓度线性范围分别为0.029 7~0.267 1 mg/mL(r=0.999 9)、 0.024 3~0.218 9 mg/mL(r=0.999 9)、0.126 0~1.134 0 mg/mL(r=0.999 9)、0.217 2~1.954 8 mg/mL(r=0.999 9)、0.136 3~1.226 9 mg/mL(r=0.999 9)、0.044 5~0.400 3 mg/mL(r=0.999 5);定量限分别为0.028 5、0.008 7、0.018 6、0.016 8、0.014 5、0.042 1 mg/mL,检测限分别为0.009 4、0.002 9、0.006 1、0.005 5、0.004 8、0.013 9 mg/mL;精密度、稳定性、重复性、耐用性试验的RSD均小于3%;加样回收率分别为98.13%~101.30%(RSD=1.20%,n=9)、98.44%~101.81%(RSD=1.28%,n=9)、98.26%~101.05%(RSD=1.19%,n=9)、99.08%~101.58%(RSD=0.89%,n=9)、98.66%~101.66%(RSD=1.17%,n=9)、98.84%~103.60%(RSD=0.96%,n=9);样品含量分别为14.552~46.766、16.951~22.096、80.597~113.115、205.224~242.537、47.761~135.697、26.493~45.771 mg/g。结论:本方法操作简便、准确,精密度、重复性良好,可用于同时测定艾纳香油中6种成分的含量,并可为艾纳香油质量的综合评价及提取工艺研究提供参考。
ABSTRACT: OBJECTIVE: To establish a method for simultaneous determination of contents of β-pinene, linalool, L-camphor, L-borneol, β-caryophyllene and xanthoxylin in the oil of Blumea balsamifera. METHODS: GC method was adopted. The determination was performed on RTX-1701 capillary column (programmed temperature). The FID detector was controlled at 240 ℃. The inlet temperature was set at 240 ℃. The carrier gas was high-purity nitrogen 3 mL/min. The the sample size was 0.5 μL, and split ratio was 50 ∶ 1. RESULTS: The linear range of β-pinene, linalool, L-camphor, L-borneol, β-caryophyllene and xanthoxylin were 0.029 7-0.267 1 mg/mL (r=0.999 9), 0.024 3-0.218 9 mg/mL (r=0.999 9), 0.126 0-1.134 0 mg/mL (r=0.999 9), 0.217 2-1.954 8 mg/mL (r=0.999 9), 0.136 3-1.226 9 mg/mL (r=0.999 9), 0.044 5-0.400 3 mg/mL(r=0.999 5), respectively. The limits of quantitation were 0.028 5, 0.008 7, 0.018 6, 0.016 8, 0.014 5, 0.042 1 mg/mL; the limits of detection were 0.009 4, 0.002 9, 0.006 1, 0.005 5, 0.004 8, 0.013 9 mg/mL, respectively. RSDs of precision, stability, reproducibility and durability tests were all lower than 3%. The average recoveries were 98.13%-101.30%(RSD=1.20%,n=9),98.44%-101.81%(RSD=1.28%,n=9),98.26%-101.05%(RSD=1.19%,n=9),99.08%-101.58%(RSD=0.89%,n=9),98.66%-101.66%(RSD=1.17%,n=9),98.84%-103.60%(RSD=0.96%,n=9), respectively. The contents of 6 components in the sample were 14.552-46.766, 16.951-22.096, 80.597-113.115, 205.224-242.537, 47.761-135.697, 26.493-45.771 mg/g, respectively. CONCLUSIONS: The established method is simple, accurate, precise and reproducible, which can be used for simultaneous determination of contents of 6 components in the oil of B. balsamifera. It can provide reference for comprehensive evaluation and extraction technology study of the oil of B. balsamifera.
期刊: 2019年第30卷第22期
作者: 陈宝雯,钱一鑫,周黎亚,王鲁,康冀川
AUTHORS: CHEN Baowen,QIAN Yixin,ZHOU Liya,WANG Lu,KANG Jichuan
关键字: 艾纳香油;气相色谱法;β-蒎烯;芳樟醇;L-樟脑;L-龙脑;β-石竹烯;花椒油素;含量测定
KEYWORDS: Oil of Blumea balsamifera; GC; β-pinene; Linalool; L-camphor; L-borneol; β-caryophyllene; Xanthoxylin; Content determination
阅读数: 109 次
本月下载数: 8 次

* 注:未经本站明确许可,任何网站不得非法盗链资源下载连接及抄袭本站原创内容资源!在此感谢您的支持与合作!