附子脂溶性生物碱与白芍总苷配伍治疗风寒湿痹证模型大鼠的增效作用研究
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篇名: 附子脂溶性生物碱与白芍总苷配伍治疗风寒湿痹证模型大鼠的增效作用研究
TITLE:
摘要: 目的:研究附子脂溶性生物碱与白芍总苷配伍治疗风寒湿痹证模型大鼠的增效作用。方法:取SD雄性大鼠100只,随机分为空白组(水),模型组(水),罗通定组[阳性对照,10.0 mg/(kg·d)]、地塞米松组[阳性对照,0.3 mg/(kg·d)],附子生物碱低、高剂量组[附子脂溶性生物碱浸膏12.5、25.0 mg/(kg·d)],白芍总苷低、高剂量组[白芍总苷粉末200.0、400.0 mg/(kg·d)],配伍低、高剂量组[附子脂溶性生物碱浸膏+白芍总苷粉末分别为12.5 mg/(kg·d)+400.0 mg/(kg·d)、25.0 mg/(kg·d)+800.0 mg/(kg·d)],每组10只。除空白组外,其余各组大鼠均于右后足垫部位注射完全弗氏佐剂0.1 mL并辅以风、寒、湿刺激来复制风寒湿痹证模型。造模19 d后开始灌胃给药或水,每日1次,持续14 d。分别于给药第0(即给药前)、3、6天时测定大鼠的关节压痛阈值(地塞米松组除外)和关节肿胀指数(罗通定组除外);采用苏木素-伊红染色法,观察大鼠(罗通定组除外)踝关节滑膜组织的病理学变化;采用酶联免疫吸附法测定大鼠(罗通定组除外)血清中抗环瓜氨酸肽(CCP)抗体并计算增效指数(CI)。结果:与空白组比较,模型组大鼠关节压痛阈值在不同给药时间点均显著降低,关节肿胀指数则显著升高(P<0.01);滑膜组织炎性病变现象明显;血清中抗CCP抗体含量显著升高(P<0.01)。与模型组比较,各给药组大鼠关节压痛阈值、关节肿胀指数、滑膜组织病变程度以及血清中抗CCP抗体含量均有不同程度的改善,大部分指标差异有统计学意义(P<0.05或P<0.01),且配伍组的指标改善效果优于单味组分给药组;基于抗CCP抗体含量计算得CI值为0.213(>1),提示两者配伍具有协同作用。结论:附子脂溶性生物碱与白芍总苷配伍治疗风寒湿痹证模型大鼠具有镇痛、抗炎、保护滑膜组织等作用,并呈协同增效作用。
ABSTRACT: OBJECTIVE: To study the efficacy enhancing effect of compatibility of Aconitum carmichaeli liposoluble alkaloids and total glucosides of peony in the treatment of wind-cold-dampness type bi-syndrome model rats. METHODS: Totally 100 SD male rats were randomly divided into blank group (water), model group (water), rotundine group [positive control, 10.0    mg/(kg·d)], dexamethasone group [positive control, 0.3 mg/(kg·d)],A. carmichaeli alkaloids low-dose and high-dose groups [A. carmichaeli  liposoluble alkaloid extract 12.5, 25.0 mg/(kg·d)], total glucosides of peony low-dose and high-dose groups [total glucosides of peony powder 200.0,400.0 mg/(kg·d)], compatibility low-dose and high-dose groups [A. carmichaeli  liposoluble alkaloid+total glucosides of peony powder were 12.5 mg/(kg·d)+400.0 mg/(kg·d), 25.0 mg/(kg·d)+800.0 mg/(kg·d) respectively], with 10 rats in each group. Except for blank group, other groups were given complete Freund’s adjuvant 0.1 mL on the right hind paw and wind-cold-dampness stimulation to induce wind-cold-dampness type bi-syndrome model. Nineteen days after modeling, they were given relevant medicine or water intragastrically, once a day, for consecutive 14 d. The joint tenderness threshold (except for dexamethasone group) and joint swelling index (except for rotundine group) were measured at 0 (before medication), 3rd and 6th day after administration respectively. HE staining was used to observe the pathological changes of synovial membrane of ankle joint in rats (except for rotundine group). ELISA assay was used to determine anti-cyclic citrullinated peptide (CCP) antibody in serum of rats (except for rotundine group), and compatibility index (CI) were calculated. RESULTS: Compared with blank group, joint tenderness threshold of rats were decreased significantly in model group at different time points, while joint swelling index was increased significantly (P<0.01). Inflammatory lesions in synovial tissue were obvious, and the content of anti-CCP antibody in serum was significantly increased (P<0.01). Compared with model group, the joint tenderness threshold, joint swelling index, synovial tissue pathological changes and anti-CCP antibody content in serum of rats in medication groups were improved in varying degrees, most of the indicators had significant difference (P<0.05 or P<0.01), and the improvement effect of the compatibility groups was better than single component treatment groups. CI value calculated based on anti-CCP antibody content was 0.213,suggesting that the compatibility of the two components had synergistic effect. CONCLUSIONS: The compatibility of A. carmichaeli liposoluble alkaloids and total glucosides of peony in the treatment of wind-cold-dampness type bi-syndrome model rats has analgesia,anti-inflammation and synovial tissue protection, with synergistic efficacy-enhancing effect.
期刊: 2019年第30卷第20期
作者: 谢运飞,李芸霞,谢红军,彭成
AUTHORS: XIE Yunfei,LI Yunxia,XIE Hongjun,PENG Cheng
关键字: 附子脂溶性生物碱;白芍总苷;配伍;风寒湿痹证;增效作用;抗环瓜氨酸肽抗体;大鼠
KEYWORDS: Aconitum carmichaeli liposoluble alkaloids;Total glucosides of peony;Compatibility; Wind-cold-dampness type bi-syndrome; Efficacy-enhancing effect; Anti-cyclic citrullinated peptide antibody; Rat
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