猴头菌多糖对溃疡性结肠炎模型大鼠肠道短链脂肪酸含量的影响
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篇名: 猴头菌多糖对溃疡性结肠炎模型大鼠肠道短链脂肪酸含量的影响
TITLE:
摘要: 目的:探讨猴头菌多糖对溃疡性结肠炎模型大鼠肠道内容物中短链脂肪酸(SCFAs)含量的影响。方法:取SD大鼠40只,随机分为空白组、模型组和猴头菌多糖低、高剂量组(0.5、1.0 g/kg),每组10只。除空白组外,其余组大鼠采用乙酸灌肠建立溃疡性结肠炎模型。造模后次日,猴头菌多糖各剂量组大鼠灌胃给予相应药液,空白组和模型组大鼠灌胃相应体积的水,连续给药10 d。取大鼠结肠组织,采用苏木精-伊红染色法观察其结肠组织病理学变化;肉眼观察大鼠结肠病变情况并进行溃疡评分,以评价疗效。采用气相色谱-质谱(GC-MS)联用法检测大鼠肠道内容物中乙酸、丙酸、丁酸、异丁酸、戊酸、异戊酸等6种SCFAs的含量。结果:与空白组比较,模型组大鼠结肠黏膜上皮组织炎性病变明显;结肠黏膜溃疡评分均显著升高(P<0.01);结肠内容物中除丙酸外的5种SCFAs含量以及SCFAs总量均显著降低(P<0.01)。与模型组比较,猴头菌多糖各剂量组大鼠结肠组织病理学损伤程度明显减轻;结肠黏膜溃疡评分均显著降低(P<0.05或P<0.01);结肠内容物中除丙酸外的5种SCFAs含量以及SCFAs总量均显著升高(P<0.05或P<0.01)。结论:猴头菌多糖能有效改善溃疡性结肠炎模型大鼠的肠黏膜病变情况;其对大鼠肠道中SCFAs(尤其是乙酸、丁酸)含量的调节作用可能是其治疗溃疡性结肠炎的重要机制。
ABSTRACT: OBJECTIVE: To investigate the effects of Hericium erinaceus polysaccharide on the content of short-chain fatty acids (SCFAs) in the intestine of ulcerative colitis model rats. METHODS: A total of 40 SD rats were randomly divided into blank group, model group and H. erinaceus polysaccharide low-dose and high-dose groups (0.5, 1.0 g/kg), with 10 rats in each group. Except for blank group, other groups were given acetic acid enema to induce ulcerative colitis model. The next day after modeling, H. erinaceus polysaccharide groups were given relevant medicine solution intragastrically; blank group and model group were given relevant volume of water intragastrically, for 10 consecutive days. Colon tissue was collected, and pathological changes of colon tissue were observed by HE staining. The pathological changes of colon in rats were observed with naked eyes and scored so as to evaluate the therapeutic efficacy. The contents of 6 kinds of SCFAs (acetic acid,propionic acid,butyric acid,isobutyric acid,pentanoic acid and isovaleric acid) in intestine of rats were determined by GC-MS. RESULTS: Compared with blank group, inflammatory lesions of colonic mucosal epithelium in model group rats were obvious; colonic mucosal ulcer score increased significantly (P<0.01); the contents of 5 kinds of SCFAs and the total amount of SCFAs in colon except for propionic acid were decreased significantly (P<0.01). Compared with model group, the degree of colonic histopathological damage in H. erinaceus polysaccharide groups was significantly reduced; colonic mucosal ulcer scores were significantly reduced (P<0.05 or P<0.01); the contents of 5 kinds of SCFAs and the total amount of SCFAs in colon except for propionic acid were increased significantly (P<0.05 or P<0.01). CONCLUSIONS: H. erinaceus polysaccharide can effectively improve the pathological condition of ulcerative colitis model rats; its regulation of intestinal SCFAs (especially acetic acid and butyric acid) may be an important mechanism of its anti-ulcerative colitis.
期刊: 2019年第30卷第20期
作者: 杨永刚,王丹丹,张艳秋,王明星
AUTHORS: YANG Yonggang,WANG Dandan,ZHANG Yanqiu,WANG Mingxing
关键字: 猴头菌多糖;溃疡性结肠炎;短链脂肪酸;机制;大鼠
KEYWORDS: Hericium erinaceus polysaccharides; Ulcerative colitis; Short-chain fatty acid; Mechanism; Rat
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