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多指标权重分析法结合正交试验优选补脾养肾颗粒的水提工艺
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| 篇名： | 多指标权重分析法结合正交试验优选补脾养肾颗粒的水提工艺 |
| TITLE： | |
| 摘要： | 目的：优选补脾养肾颗粒的水提工艺，为其后续开发研究提供依据。方法：分别采用高效液相色谱-蒸发光散射检测器（HPLC-ELSD）法和高效液相色谱-二极管阵列检测（HPLC-DAD）法测定补脾养肾颗粒的水提液中黄芪甲苷和丹酚酸B的含量，以黄芪甲苷、丹酚酸B的含量以及出膏率的综合评分为指标，采用层次分析（AHP）法、指标重复性相关（CRITIC）法和AHP-CRITIC 混合加权法确定各指标权重系数，设计L9（34）正交试验筛选补脾养肾颗粒的水提工艺中煎煮时间、加水倍数和煎煮次数，并进行验证试验。结果：以AHP-CRITIC混合加权法确定的权重系数最为合理，筛选的提取工艺为药材煎煮2次，每次加12倍量水、煎煮1 h。3次验证试验结果显示，黄芪甲苷、丹酚酸B的平均含量分别为8.79、609.50 mg（整方121 g药材提取得到的总量），平均出膏率为31.24％，平均综合评分为96.59分（RSD＝1.01％，n＝3）。结论：优选的水提工艺重复性好、稳定可行，可为补脾养肾颗粒的后续开发和工业化生产提供科学依据。 |
| ABSTRACT： | OBJECTIVE： To optimize the water extraction technology of Bupi yangshen granules， and to provide basis for the follow-up research and development of it. METHODS： The contents of astragaloside Ⅳ and salvianolic acid B in water extract of Bupi yangshen granules， were determined by HPLC-ELSD and HPLC-DAD. Using the comprehensive score of contents of astragaloside Ⅳ and salvianolic acid B and extract yield as index， weight coefficient of indicators were determined by AHP， CRITIC and AHP-CRITIC mixed weighting method. L9（34） orthogonal test was used to optimize decoction time， water volume and decoction times in water extraction technology of Bupi yangshen granules. Validation test was also performed. RESULTS： The weight coefficient determined by AHP-CRITIC mixed weighting method was the most reasonable. The optimal extraction technology was decocting twice， adding 12-fold water， 1 h each time. The results of 3 times of validation test showed that the average contents of astragaloside Ⅳ and salvianolic acid B were 8.79， 609.50 mg （total amount of 121 g medicinal herbs extracted from whole prescription）， respectively. The average extract yield was 31.24％. Average comprehensive score was 96.59（RSD＝1.01％，n＝3）. CONCLUSIONS： The optimized water extraction technology is reproducible， stable and feasible. It can provide a scientific basis for the follow-up development and industrial production of Bupi yangshen granules. |
| 期刊： | 2019年第30卷第19期 |
| 作者： | 胡兆流，陈秋谷，王佛长，黄诗莹，郑平，张尚斌，易铁钢，李顺民，陈剑平 |
| AUTHORS： | HU Zhaoliu，CHEN Qiugu，WANG Fochang，HUANG Shiying，ZHENG Ping，ZHANG Shangbin，YI Tiegang，LI Shunmin，CHEN Jianping |
| 关键字： | 补脾养肾颗粒；水提工艺；正交试验；多指标权重分析；层次分析-指标重复性相关混合加权法 |
| KEYWORDS： | Bupi yangshen granules； Water extraction technology； Orthogonal test； Multi-index weighting analysis method； AHP-CRITIC mixed weighting method |
| 阅读数： | 317 次 |
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