野生化血丹不同部位中梓醇与桃叶珊瑚苷含量的比较
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篇名: 野生化血丹不同部位中梓醇与桃叶珊瑚苷含量的比较
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摘要: 目的:测定并比较野生化血丹不同部位(根、茎、叶、花)中梓醇与桃叶珊瑚苷的含量,为其药用部位选择和资源开发提供参考。方法:建立高效液相色谱法(HPLC)对野生化血丹根、茎、叶、花等部位中梓醇和桃叶珊瑚苷含量进行测定,并对不同部位中含量进行比较分析。梓醇测定的色谱柱为Agilent TC-C18,流动相为甲醇-0.1%磷酸溶液(1 ∶ 99,V/V),检测波长为210 nm,流速为1 mL/min,进样量为20 μL,柱温为35 ℃;桃叶珊瑚苷测定的色谱柱为SPHERI-5RP-C18,流动相为乙腈-水(3 ∶ 97,V/V),检测波长为205 nm,流速为1 mL/min,进样量为20 μL,柱温为25 ℃。结果:梓醇和桃叶珊瑚苷分别在进样质量浓度为0.061 5~3.321、0.000 36~0.216 mg/mL范围内与其各自峰面积呈良好的线性关系(r均为0.999 9);检测限分别为0.016、0.007 μg/mL;定量限分别为0.052、0.023 μg/mL;精密度、稳定性(24 h)、重复性试验的RSD均<2.00%(n=6);平均回收率分别为99.34%、99.61%,RSD分别为1.06%、1.12%(n=6)。含量测定结果显示,野生化血丹根、茎、叶和花中梓醇的含量分别为1.609、3.030、11.095和1.921 mg/g,桃叶珊瑚苷的含量分别为0.441、0.020、0.005和0.006 mg/g。结论:所建立的HPLC法符合定量分析要求。梓醇主要分布在野生化血丹的叶中,桃叶珊瑚苷主要分布在野生化血丹的根中;该研究结果可为合理选择野生化血丹不同药用部位作为原材料开发不同功效的药物提供参考。
ABSTRACT: OBJECTIVE: To determine and compare the contents of catalpol and aucubin in different parts (root, stem, leaf and flower) of wild Centranthera grandiflora, and to provide reference for the selection of medicinal parts and source development. METHODS: HPLC method was used to determine the contents of catalpol and aucubin in root, stem, leaf and flower of wild C. grandiflora, and the contents of different parts were analyzed comparatively. The determination of catalpol was performed on Agilent TC-C18 column with mobile phase consisted of methanol-0.1% phosphoric acid (1 ∶ 99, V/V) at the flow rate of 1 mL/min; the detection wavelength was set at 210 nm, and sample size was 20 μL. The column temperature was 35 ℃; the determination of aucubin was performed on SPHERI-5RP-C18 column with mobile phase consisted of acetonitrile-water (3 ∶ 97, V/V) at the flow rate of 1 mL/min; the detection wavelength was set at 205 nm, and sample size was 20 μL; the column temperature was 25 ℃. RESULTS: The linear range of catalpol and aucubin were 0.061 5-3.321 and 0.000 36-0.216 mg/mL (all r=0.999 9). The limits of detection were 0.016 and 0.007 μg/mL. The limits of quantitation were 0.052 and 0.023 μg/mL. RSDs of precision, stability (24 h) and reproducibility tests were all lower than 2.00% (n=6). The average recoveries were 99.34% and 99.61%, and RSDs were 1.06% and 1.12%, respectively (n=6). The average content of catalpol in root, stem, leaf and flower wild C. grandiflora were 1.609, 3.030, 11.095 and 1.921 mg/g, respectively. The contents of aucubin in different parts were 0.441, 0.020, 0.005 and 0.006 mg/g,respectively. CONCLUSIONS:The established HPLC method meets the requirements of quantitative analysis. Catalpol is mainly distributed in the leaves of wild C. grandiflora, and aucubin is mainly distributed in the roots of wild C. grandiflora. The experimental conclusion provides a reference for the reasonable selection of different medicinal parts as raw materials to develop medicine with different efficacy.
期刊: 2019年第30卷第19期
作者: 张传利,李维峰,马晓,朱春梅,赵秀,杜华波,陈治华,何素明
AUTHORS: ZHANG Chuanli,LI Weifeng,MA Xiao,ZHU Chunmei,ZHAO Xiu,DU Huabo,CHEN Zhihua,HE Suming
关键字: 野生化血丹;梓醇;桃叶珊瑚苷;高效液相色谱法;药用部位;含量测定
KEYWORDS: Wild Centranthera grandiflora; Catalpol; Aucubin; HPLC; Medicinal part; Content determination
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