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5味山姜属中药不同极性部位对胃溃疡寒证模型大鼠交感神经-肾上腺系统机能的影响
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| 篇名： | 5味山姜属中药不同极性部位对胃溃疡寒证模型大鼠交感神经-肾上腺系统机能的影响 |
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| 摘要： | 目的：探讨高良姜、大高良姜、红豆蔻、草豆蔻、益智等5味山姜属中药不同极性部位对胃溃疡寒证大鼠交感神经-肾上腺系统机能的影响。方法：取SD大鼠，均灌胃4 ℃知母水煎液、冰醋酸溶液复制胃溃疡寒证模型，并于造模后随机分为模型组、附子理中丸组（阳性对照，9.0 g/kg）、西咪替丁组（阳性对照，0.003 3 g/kg）以及5种中药石油醚、乙酸乙酯、正丁醇、水萃取部位低、高剂量组（以下简称“高石低/高”“大石低/高”“红石低/高”“草石低/高”“益石低/高”“高乙低/高”“大乙低/高”“红乙低/高”“草乙低/高”“益乙低/高”“高正低/高”“大正低/高”“红正低/高”“草正低/高”“益正低/高”“高水低/高”“大水低/高”“红水低/高”“草水低/高”“益水低/高”，剂量分别为0.064/0.256、0.032/0.128、0.008/0.032、0.075/0.3、0.1/0.4、0.064/0.256、0.108/0.432、0.16/0.64、0.064/0.25、0.125/0.5、0.056/0.224、0.108/0.432、0.08/0.32、0.2/0.8、0.3/1.2、0.14/0.56、0.032/0.128、0.028/0.112、0.05/0.2、0.087/0.348 g/kg，以萃取部位质量计），每组10只；另设空白组（常温水）。造模次日，空白组和模型组大鼠均灌胃等容常温水，各给药组大鼠灌胃相应药液2 mL/100 g，q12 h，共给药4次。末次给药后，采用酶联免疫吸附测定法检测各组大鼠尿液中17-羟皮质类固醇（17-OHCS）和儿茶酚胺类（CAs）物质[肾上腺素（A）、去甲肾上腺素（NE）、多巴胺（DA）]以及血清中促肾上腺皮质素（ACTH）、多巴胺-β-羟化酶（D-β-H）的含量。结果：与空白组比较，模型组大鼠尿液中17-OHCS、A、NE、DA以及血清中ACTH、D-β-H含量均显著降低（P＜0.01）。与模型组比较，不同给药组大鼠尿液17-OHCS（附子理中丸组、西咪替丁组以及高石低/高、大石高、红石高、草石高、益石高组，5种药材乙酸乙酯部位各剂量组，大正高、红正高、草正高、益正高组，高水高、大水高、红水高组）、A（附子理中丸组、西咪替丁组以及高石高、大石高、红石低/高、草石低/高、益石高组，5种药材乙酸乙酯部位各剂量组，高正高、大正高、红正低/高、草正高、益正高组，红水高组）、NE（附子理中丸组、西咪替丁组，5种药材石油醚部位、乙酸乙酯部位、正丁醇部位各剂量组，以及高水高、大水高、红水高组）、DA[附子理中丸组、西咪替丁组，5种药材石油醚部位各剂量组（除高石低外），乙酸乙酯部位各剂量组，以及高正高、大正高、红正高、草正高、益正高组，红水高组]含量和血清ACTH[附子理中丸组，5种药材石油醚部位各剂量组（除高石低、大石低、草石低组外）、乙酸乙酯部位各剂量组（除大乙低、红乙低组外）、红正高组、大水高组]、D-β-H[附子理中丸组以及高石高、大石高、红石低/高、益石高组，5种药材乙酸乙酯部位各剂量组（除大乙低、红乙低、益乙低组外），大正高、红正高组、红水高组]含量均显著升高（P＜0.05或P＜0.01）；且大石高组17-OHCS、D-β-H含量，草石高组ACTH、D-β-H含量以及高石高、益石高组D-β-H含量均显著低于红石高组；高乙高、草乙高、益乙高组DA含量以及高乙高、红乙高、草乙高、益乙高组D-β-H含量均显著低于大乙高组，高乙高、草乙高组DA含量均显著低于红乙高组，高乙高、大乙高、草乙高、益乙高组ACTH含量均显著高于红乙高组；高正高、大正高、草正高、益正高组17-OHCS、DA、ACTH含量，大正高、草正高、益正高组A含量，高正高、草正高、益正高组D-β-H含量均显著低于红正高组；草水高、益水高组17-OHCS、D-β-H含量均显著低于大水高组；草水高、益水高组17-OHCS、DA、D-β-H含量和高水高组DA、D-β-H含量均显著低于红水高组（P＜0.05或P＜0.01）。结论：5味山姜属中药的不同极性部位对胃溃疡寒证模型大鼠具有不同程度的改善作用，其中红豆蔻最佳，高良姜次之；上述中药均可通过增加17-OHCS、CAs、D-β-H的含量来调节交感神经-肾上腺系统的机能。 |
| ABSTRACT： | OBJECTIVE： To investigate the effects of different polar parts of 5 kinds of Alpinia on the function of sympathetic-adrenal system in gastric ulcer model rats with cold syndrome， such as the root of Alpinia officinarum， the rhizome and fruit of Alpinia galangal， the seed of Alpinia katsumadai， and the fruit of Alpinia oxyphylla. METHODS： SD rats were given Anemarrhena asphodeloides decoction at 4 ℃ and Glacial acetic acid solution intragastrically to induce gastric ulcer model with cold syndrome； the model rats were randomly divided into model group， Fuzi lizhong pills group （positive control， 9.0 g/kg）， cimetidine group （positive control， 0.003 3 g/kg）， low-dose and high-dose groups of petroleum ether， ethyl acetate， n-butanol and water extraction parts from 5 medicinal materials （hereinafter referred to as “gaoshidi” “gaoshigao” “dashidi” “dashigao” “hongshidi” “hongshigao” “caoshidi” “caoshigao” “yishidi” “yishigao” “gaoyidi” “gaoyigao” “dayidi” “dayigao” “hongyidi” “hongyigao” “caoyidi” “caoyigao” “yiyidi” “yiyigao” “gaozhengdi” “gaozhenggao” “dazhengdi” “dazhenggao” “hongzhengdi” “hongzhenggao” “caozhengdi” “caozhenggao” “yizhengdi” “yizhenggao” “gaoshuidi” “gaoshuigao” “dashuidi” “dashuigao” “hongshuidi” “hongshuigao” “caoshuidi” “caoshuigao” “yishuidi” “yishuigao”， 0.064/0.256， 0.032/ 0.128， 0.008/0.032， 0.075/0.3， 0.1/0.4， 0.064/0.256， 0.108/0.432， 0.16/0.64， 0.064/0.25， 0.125/0.5， 0.056/0.224， 0.108/0.432， 0.08/0.32， 0.2/0.8， 0.3/1.2， 0.14/0.56， 0.032/0.128， 0.028/0.112， 0.05/0.2， 0.087/0.348 g/kg， by mass of extraction parts）， with 10 rats in each group； and the blank group （normal temperature water） was set up. Next day after modeling， blank group and model group were given constant volume of normal temperature water intragastrically； administration group was given relevant solution 2 mL/100 g intragastrically， q12 h， 4 times in total. After last medication， urine contents of 17-OHCS， CAs substances （A， NE， DA） and serum contents of ACTH and D-β-H were determined by ELISA. RESULTS： Compared with blank group， the contents of 17-OHCS， A， NE and DA in urine， the contents of ACTH and D-β-H in serum were decreased significantly in model group （P＜0.01）. Compared with model group， the contents of 17-OHCS （Fuzi lizhong pills group， cimetidine group， gaoshidi， gaoshigao， dashigao， hongshigao， caoshigao and yishigao groups， ethyl acetate part groups of 5 medicinal materials， dazhenggao， hongzhenggao， caozhenggao and yizhenggao groups， gaoshuigao， dashuigao and hongshuigao groups）， A （Fuzi lizhong pills group， cimetidine group， gaoshigao， dashigao， hongshidi， hongshigao， caoshidi， caoshigao and yishigao groups， ethyl acetate part groups of 5 medicinal materials， gaozhenggao， dazhenggao， hongzhengdi， hongzhenggao， caozhenggao and yizhenggao groups， hongshuigao group）， NE （Fuzi lizhong pills group， cimetidine group， petroleum ether part， ethyl acetate part and n-butanol part groups of 5 medicinal materials， gaoshuigao， dashuigao and hongshuigao groups）， DA [Fuzi lizhong pills group， cimetidine group， petroleum ether part （except for gaoshidi group） and ethyl acetate part groups of 5 medicinal materials， gaozhenggao， dazhenggao， hongzhenggao， caozhenggao and yizhenggao groups， hongshuigao group] in urine， serum contents of ACTH [Fuzi lizhong pills group， petroleum ether part （except for gaoshidi， dashidi and caoshidi groups） and ethyl acetate part （except for dayidi and hongyidi groups） groups of 5 medicinal materials， hongzhenggao group， dashuigao group]， and D-β-H [Fuzi lizhong pills group， gaoshigao， dashigao， hongshidi， hongshigao and yishigao groups， ethyl acetate part groups of 5 medicinal materials （except for dayidi， hongyidi and yiyidi groups）， dazhenggao and hongzhenggao groups， hongshuigao group] were increased significantly （P＜0.05 or P＜0.01）. The contents of 17-OHCS and D-β-H in dashigao group， the contents of ACTH and D-β-H in caoshigao group and the contents of D-β-H in gaoshigao and yishigao groups were significantly lower than hongshigao group. The contents of DA in gaoyigao， caoyigao and yiyigao groups as well as the contents of D-β-H in gaoyigao， hongyigao， caoyigao and yiyigao groups were significantly lower than dayigao group； the contents of DA in gaoyigao and caoyigao groups were significantly lower than hongyigao group， the contents of ACTH in gaoyigao， dayigao， caoyigao and yiyigao groups were significantly higher than hongyigao group. The contents of 17-OHCS， DA and ACTH in gaozhenggao， dazhenggao， caozhenggao and yizhenggao groups， the contents of A in dazhenggao， caozhenggao and yizhenggao groups as well as the contents of D-β-H in gaozhenggao， caozhenggao and yizhenggao groups were significantly lower than hongzhenggao group. The contents of 17-OHCS and D-β-H in caoshuigao and yishuigao groups were significantly lower than dashuigao group. The contents of 17-OHCS， DA and D-β-H in caoshuigao and yishuigao groups as well as the contents of DA and D-β-H in gaoshuigao groups were significantly lower than hongshuigao group （P＜0.05 or P＜0.01）. CONCLUSIONS： Different polar parts of 5 kinds of Alpinia can improve gastric ulcer model rats with cold syndrome to different extents； among them， the fruit of A. galangal is the best， followed by the root of A. officinarum. Above medicinal materials can regulate the function of sympathetic-adrenal system by increasing the contents of 17-OHCS， CAs and D-β-H. |
| 期刊： | 2019年第30卷第18期 |
| 作者： | 柳俊辉，丘海冰，谢鹏，苏善美，李明芳，陈俊其，尹优，秦华珍 |
| AUTHORS： | LIU Junhui，QIU Haibing，XIE Peng，SU Shanmei，LI Mingfang，CHEN Junqi，YIN You，QIN Huazhen |
| 关键字： | 山姜属中药；极性部位；胃溃疡寒证；交感神经-肾上腺系统；机能；大鼠 |
| KEYWORDS： | Alpinia； Polar part； Gastric ulcer with cold syndrome； Sympathetic-adrenal system； Function； Rat |
| 阅读数： | 186 次 |
| 本月下载数： | 3 次 |
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