壮通饮对冠心病模型大鼠心功能及血管舒缩功能的影响
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篇名: 壮通饮对冠心病模型大鼠心功能及血管舒缩功能的影响
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摘要: 目的:观察壮通饮水提物对冠心病(CHD)模型大鼠心功能及血管舒缩功能的影响及其可能机制。方法:将SD大鼠随机分为对照组、假手术组、模型组、阳性组(复方丹参滴丸,0.08 g/kg)以及壮通饮水提物低、中、高剂量组(6.8、13.6、27.2 g/kg,以水提物浸膏质量计),每组10只。除对照组(不手术)和假手术组(只开胸不结扎)外,其余各组大鼠均通过结扎左心室回旋支末梢建立CHD模型。造模1周后,对照组、假手术组和模型组大鼠均灌胃等体积水,各给药组大鼠均灌胃相应药物;每日1次,连续4周。末次给药2 h后,检测各组大鼠心功能指标[左室舒张末期容积(LVEDV)、左室收缩末期容积(LVESV)、每搏量(SV)、左室射血分数(LVEF)],采用酶联免疫吸附测定法检测其心肌组织炎症因子[C反应蛋白(CRP)、白细胞介素1β(IL-1β)、干扰素γ(IFN-γ)]和内皮素1(ET-1)水平,采用苏木精-伊红染色法观察其心肌组织形态学特征。结果:对照组和假手术组大鼠心肌纤维结构清晰、排列整齐,两组大鼠各项指标比较差异均无统计学意义(P>0.05)。与对照组比较,模型组大鼠心肌纤维排列紊乱,可见断裂、溶解、坏死等现象,并伴有炎症细胞浸润,其LVEDV、LVESV以及CRP、IL-1β、ET-1、IFN-γ水平均显著升高,SV、LVEF均显著下降(P<0.05或P<0.01)。与模型组比较,各给药组大鼠上述症状均有不同程度的改善,其中阳性组大鼠LVEDV、LVESV和CRP、IL-1β、ET-1、IFN-γ水平,壮通饮水提物中、高剂量组大鼠LVEDV以及壮通饮水提物各剂量组大鼠LVESV和CRP、IL-1β、ET-1、IFN-γ水平均显著降低,阳性组大鼠SV、LVEF,壮通饮水提物各剂量组大鼠SV以及壮通饮水提物中、高剂量组大鼠LVEF均显著升高(P<0.05或P<0.01)。结论:壮通饮水提物可通过作用于炎症环节而降低ET-1水平,从而改善CHD模型大鼠的血管舒缩功能,恢复其心肌组织血液供应,改善其心功能。
ABSTRACT: OBJECTIVE: To observe the effects of Zhuangtongyin (ZTY) water extract on blood vessels diastolic and systolic function and cardiac function in rats with coronary heart disease (CHD) and its potential mechanism. METHODS: SD rats were randomly divided into control group, sham operation group, model group, positive group (Compound danshen dripping pills, 0.08 g/kg) and ZTY water extract low-dose, medium-dose and high-dose groups (6.8, 13.6, 27.2 g/kg, by weight of water extract), with 10 rats in each group. Except for control group (no operation) and sham operation group (only open chest without ligation), CHD model were set up in other groups by ligating the left ventricle cyclotron endings. One week after modeling, control group, sham operation group and model group were given constant volume of water intragastrically, and administration groups were given relevant medicine intragastrically; once a day, for consecutive 4 weeks. Two hours after last administration, cardiac function indexes (LVEDV, LVESV, SV, LVEF) of rats were detected in each group. The levels of inflammatory factors (CRP, IL-1β, IFN-γ and ET-1) in myocardial tissue were determined by ELISA. Histomorphological characteristics of myocardial tissue were determined by HE staining. RESULTS: The structure of myocardial fibers in control group and sham operation group was clear and orderly; there was no statistical significance in difference of each index between 2 groups (P>0.05). Compared with control group, myocardial fibers were disorderly arranged in model group, and the phenomena of rupture, dissolution and necrosis were observed, accompanied by infiltration of inflammatory cells. The levels of LVEDV and LVESV as well as the levels of CRP, IL-1β, ET-1 and IFN-γ were increased significantly, while the levels of SV and LVEF were decreased significantly (P<0.05 or P<0.01). Compared with model group, above symptoms of rats in each administration group were improved to different extents; the levels of LVEDV and LVESV as well as the levels of CRP, IL-1β, ET-1 and IFN-γ in positive group, the levels of LVEDV in ZTY water extract medium-dose and high-dose groups, the levels of LVESV and CRP, IL-1β, ET-1, IFN-γ in ZTY water extract groups were decreased significantly. The levels of SV and LVEF in positive group, the levels of SV in ZTY water extract groups as well as the levels of LVEF in ZTY water extract medium-dose and high-dose groups were increased significantly (P<0.05 or P<0.01). CONCLUSIONS: ZTY water extract could reduce the level of ET-1 and improve blood vessels diastolic and systolic function in CHD model rats, then restore blood supply of myocardial tissue and strengthen cardiac function, by acting on inflammation cycle.
期刊: 2019年第30卷第18期
作者: 张世田,庞路路,唐汉庆,黄小珊,冯悦,黄岑汉
AUTHORS: ZHANG Shitian,PANG Lulu,TANG Hanqing,HUANG Xiaoshan,FENG Yue,HUANG Cenhan
关键字: 壮通饮;水提物;冠心病;心功能;血管舒缩功能;炎症因子;内皮素1;大鼠
KEYWORDS: Zhuangtongyin; Water extract; Coronary heart disease; Cardiac function; Blood vessels diastolic and systolic function; Inflammatory factor; ET-1; Rat
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