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Box-Behnken响应面法优化管花肉苁蓉中毛蕊花糖苷的提取工艺研究
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| 篇名： | Box-Behnken响应面法优化管花肉苁蓉中毛蕊花糖苷的提取工艺研究 |
| TITLE： | |
| 摘要： | 目的：优化管花肉苁蓉中毛蕊花糖苷的提取工艺，为该药材的深入开发和综合利用提供参考。方法：采用高效液相色谱法对管花肉苁蓉中的毛蕊花糖苷进行含量测定。色谱柱为Inertsil-ODS-3V，流动相为甲醇-0.2％甲酸水溶液（40 ∶ 60，V/V），流速为1 mL/min，柱温为30 ℃，检测波长为330 nm，进样量为10 μL。以毛蕊花糖苷的提取率为考察指标，分别对浸泡时间、乙醇浓度、液料比、提取时间、提取次数进行单因素试验；根据上述试验结果采用Box-Behnken响应面法对乙醇浓度、液料比、提取时间等条件进行优化，并对优化后的提取工艺进行验证试验。结果：毛蕊花糖苷检测质量浓度的线性范围为18.65～932.4 μg/mL。最优提取工艺为乙醇浓度63％、液料比8 ∶ 1（mL/g）、浸泡2 h、提取时间1.5 h、提取2次。3次平行验证试验所得毛蕊花糖苷的提取率分别为78.21％、76.95％、79.34％，与预测值76.76％的相对误差分别为1.89％、0.25％、3.36％。结论：优化所得管花肉苁蓉中毛蕊花糖苷的提取工艺稳定、可行，适用于该化合物的提取。 |
| ABSTRACT： | OBJECTIVE： To optimize the extraction technology of verbascoside from Cistanche tubulosa， and to provide reference for further development and comprehensive utilization of C. tubulosa. METHODS： The content of verbascoside in C. tubulosa was determined by HPLC. The determination was performed on Inertsil-ODS-3V column with mobile phase consisted of methanol-0.2％ formic acid aqueous solution （40 ∶ 60， V/V） at the flow rate of 1 mL/min. The column temperature was 30 ℃， the detection wavelength was 330 nm， and the sample size was 10 μL. Using extraction rate of verbascoside as index， soaking time， ethanol concentration， liquid-solid ratio， extraction time and extraction times were investigated by single factor tests. According to the results of above tests， ethanol concentration， liquid-solid ratio and extraction time were optimized by Box-Behnken response surface methodology. The verification tests were carried out on the optimized extraction technology. RESULTS： The linear range of verbascoside was 18.65-932.4 μg/mL. The optimal extraction technology included that ethanol concentration 63％， liquid-solid ratio 8 ∶ 1 （mL/g）， soaking for 2 h， extraction time 1.5 h， extracting for 2 times. The extraction rates of verbascoside in the three parallel verification tests were 78.21％， 76.95％， 79.34％， respectively. The relative errors of those to predicted value 76.76％ were 1.89％， 0.25％， 3.36％. CONCLUSIONS： The optimized extraction technology of verbascoside from C. tubulosa is stable and feasible， and is suitable for the extraction of verbascoside. |
| 期刊： | 2019年第30卷第14期 |
| 作者： | 孔征，毛乐静，霍仕霞，苏娅，姜萌，闫明 |
| AUTHORS： | KONG Zheng，MAO Lejing，HUO Shixia，SU Ya，JIANG Meng，YAN Ming |
| 关键字： | 管花肉苁蓉；毛蕊花糖苷；响应面法； Box-Behnken 设计；提取工艺；优化 |
| KEYWORDS： | Cistanche tubulosa； Verbascoside； Response surface methodology； Box-Behnken design； Extraction technology； Optimization |
| 阅读数： | 436 次 |
| 本月下载数： | 9 次 |
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